134 physicians have called on the Department of Health and Human Services and the U.S. Department of Agriculture to prioritize beans, peas, and lentils as a protein source in the next Dietary Guidelines for Americans.
In a letter sent on June 24, the doctors — all members of the Physicians Committee for Responsible Medicine (PCRM) — said that promoting legume consumption would help to prevent and reduce chronic disease. In contrast, they claim that red and processed meats are strongly associated with cardiovascular disease, diabetes, and certain cancers.
Furthermore, legumes are whole foods and are rich in fiber, which most Americans are deficient in. Some types are sourced from American farmers, who could benefit from an increase in legume consumption.
Photo: Polina Tankilevitch on Pexels
“Important and appropriate emphasis”
Recently, the 2025 Dietary Guidelines Advisory Committee recommended that federal nutrition guidelines be modified to move legumes from the Vegetables Food Group to the Protein Food Group. It also suggested that Beans, Peas, and Lentils should be listed as the first protein subgroup, followed by Nuts, Seeds, and Soy Products. Seafood would come third, while Meats, Poultry, and Eggs would be last on the list.
In their letter, the PCRM physicians support these recommendations, noting that the reorganization would “more accurately classify these foods as a major protein source in many Americans’ diets”. They also say the change would educate people on the nutritional value of legumes, while dispelling the myth that plant-based foods are an incomplete source of protein.
“The Dietary Guidelines Advisory Committee’s report put important and appropriate emphasis on beans and other plant-based foods,” said Neal Barnard, MD, FACC, president of the Physicians Committee. “Overwhelming evidence supports the role of these foods in supporting cardiovascular health, promoting a healthy body weight, and reducing the risk of type 2 diabetes, cancer, and other serious conditions. It is vital that the next Dietary Guidelines for Americans prioritize these nutritious sources of protein.”
The PRISMA flow diagram illustrating the literature search process is presented in Fig. 1. Based on a systematic search across the specified databases, a total of 3,245 articles were initially retrieved. These included 539 articles from Embase, 1037 from PubMed, 847 from Scopus, 770 from Web of Science, and 16 from ProQuest. After removing 1388 duplicate records, two researchers independently screened the titles and abstracts of 1857 articles. Of these, 1,806 articles were excluded due to non-compliance with the inclusion and exclusion criteria. The full texts of 51 articles were assessed, and ultimately, 27 primary studies were selected for data extraction [27, 29, 42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65,66]. The remaining 24 articles were excluded for the following reasons: they did not focus on burn injuries, lacked direct or indirect data extraction possibilities, used alternative effect size metrics, did not utilize MSCs, or employed bilayered scaffolds.
Fig. 1
PRISMA flow diagram detailing study screening and selection
Study characteristics
Animal models
Among the included studies, 14 studies utilized mice, 11 studies employed rats, one study used rabbits, and one study involved pigs (Fig. 2A). Of these, 16 studies focused on male animals, six studies on female animals, and one study included both sexes (Fig. 2B). The sex of the animals was not reported in four studies. Regarding the burn models, 16 studies induced third-degree burns, nine studies created second-degree burns, and the burn model was not specified in two studies (Fig. 2C). The methods employed to induce burns, along with other relevant information pertaining to the animal model, are concisely presented in Table 1.
Fig. 2
Comperhensive overview of included study characteristics
Mesenchymal stem cells
Out of the 20 studies that utilized MSCs, 11 studies employed animal-derived MSCs, while nine studies used human-derived MSCs (Fig. 2D). Specifically, 11 studies used adipose tissue-derived MSCs, six studies utilized bone marrow-derived MSCs, and three studies employed umbilical cord-derived MSCs (Fig. 2E). A summary of the points mentioned, along with the methods for identifying and characterizing MSCs based on ISCT guidelines [67], is provided in Table 2.
Secretome
According to Table 3, among the seven studies that isolated MSCs, three studies focused on exosomes, one study investigated microvesicles, one study examined small extracellular vesicles, one study analyzed paracrine proteins, and one study utilized conditioned medium (Fig. 2F). Of these, four studies investigated secretomes derived from umbilical cord MSCs, two studies explored secretomes from adipose-derived MSCs, and one study used secretomes from iPSC-derived MSCs (Fig. 2G). For isolation, four studies employed ultrafiltration, and three studies used ultracentrifugation as the primary methods.
Scaffold types
In terms of scaffolds, six studies utilized biological-based scaffolds, while 21 studies employed hydrogel-based scaffolds (Fig. 2H). The hydrogel formulations included injectable hydrogels, foam hydrogels, amorphous hydrogels, film hydrogels, freeze-dried hydrogels, nanofiber hydrogels, and bioprinted hydrogels (Fig. 2I). The most commonly used biomaterials in these hydrogels were hyaluronic acid, collagen, chitosan, gelatin, and alginate (Fig. 2J). The information provided pertains to scaffolds, including Scaffold Compositions, Forms, and characterization methods, as well as the Structural and Biological Properties of scaffolds, all of which are comprehensively presented in Table 4.
Result of risk of bias assessment
The risk of bias assessment, conducted using the SYRCLE tool, yielded the following findings across the included studies. In the domain of selection bias, under the subcategory of sequence generation, 18 out of 27 studies (66.7%) were classified as having a low risk of bias. In the baseline characteristics subcategory, all but one study—i.e., 26 out of 27 studies (96.3%)—were deemed to have a low risk of bias. However, in the allocation concealment subcategory, all studies were rated as having an unclear risk of bias. In the domain of performance bias, the random housing subcategory indicated that 15 out of 27 studies (55.6%) were at low risk of bias, whereas in the blinding subcategory, all studies were assessed as having an unclear risk of bias. Regarding detection bias, the random outcome assessment subcategory showed that, with the exception of three studies, the remaining 24 out of 27 studies (88.9%) were classified as low risk. In contrast, the blinding subcategory within this domain revealed that all studies had an unclear risk of bias.In the domain of attrition bias, all but three studies—i.e., 24 out of 27 studies (88.9%)—were determined to have a low risk of bias. Finally, in the domain of reporting bias, all studies were consistently rated as having a low risk of bias. In total, of the 27 studies evaluated, 19 studies (70.4%) were classified as having an overall low risk of bias and were included in our review, while the remaining 8 studies (29.6%) were deemed to have an unclear risk of bias (Fig. 3). Also, to examine the impact of studies with unclear risk of bias on potential under- or overestimation of results, a subgroup analysis was conducted for the primary outcome. As shown in Table 5 and (Supplementary Data S2), these studies did not significantly influence the overall findings.
Fig. 3
Quality assessment of individual studies using SYstematic Review Center for Laboratory animal Experimentation (SYRCLE) tool
Primary outcome
Our analysis demonstrates that the synergistic effect of MSCs and scaffolds significantly improves wound closure rates, measured as the primary outcome, across three time frames. To evaluate the progression of wound healing, these time points were categorized as short-term (1-week), mid-term (2 weeks), and long-term (3 weeks). These intervals align with the inflammatory, proliferative, and remodeling phases of the wound healing process, respectively, and were selected based on standard practices in preclinical burn wound research. Moreover, these time points were chosen due to their frequent reporting in the scientific literature, enabling standardization and comparison of results across studies. These results are derived from 23 studies for the 1-week time point, 25 studies for 2 weeks, and 12 studies for 3 weeks. The effect was most pronounced at 1 week (SMD = 3.97, 95% CI: 2.92 to 5.01), followed by 2 weeks (SMD = 3.47, 95% CI: 2.23 to 4.61), and 3 weeks (SMD = 3.03, 95% CI: 1.96 to 4.11) (Fig. 4A–C). These results indicate that the combination of MSCs and scaffolds is highly effective in promoting wound healing, with the strongest impact observed in the early stages. However, the high heterogeneity (I2 > 50%) across studies suggests variability in experimental conditions, which should be considered when interpreting these findings.
Fig. 4
Forest plot demonstrating the therapeutic efficacy of MSC-scaffold combinations in promoting wound closure in a burn animal model. A Week 1, B Week 2, C Week 3
Subgroup analysis and meta-regression
Due to the presence of high heterogeneity and to investigate its underlying causes as well as identify factors influencing therapeutic efficacy, we conducted a subgroup analysis across three time frames: 1 week, 2 weeks, and 3 weeks. The results revealed noteworthy findings (all results are available in Table 5 and Supplementary Data S2). Our results indicate that, in the comparison between the use of MSCs and MSC-derived secretome, the administration of MSCs (SMD = 4.75, 95% CI: 3.15 to 6.36) demonstrated superior therapeutic efficacy in the short term (1-week) compared to secretome (SMD = 2.90, 95% CI: 1.77 to 4.03). However, in the medium term (2-week) and long term (3-week), specifically in 2-week the therapeutic efficacy of secretome (SMD = 3.94, 95% CI: 2.30 to 5.58) was greater than that of MSCs (SMD = 3.20, 95% CI: 1.71 to 4.68). I2 analysis in this subgroup suggests that one of the main sources of heterogeneity was the inclusion of MSCs and MSC-derived secretome combined with scaffolds. Separate analysis of these combinations reduced heterogeneity; however, due to the limited number of secretome studies, we included both scaffold-based MSC and secretome data in the pooled results. The investigation of the therapeutic efficacy of MSCs across various animal models indicates a significant reduction in I2 (heterogeneity). This suggests that the choice of animal model utilized in the studies may be one of the key factors contributing to the heterogeneity observed in our results. The evaluation of the therapeutic efficacy of MSCs in second- and third-degree burn models reveals significant findings. In the one-week time frame, the wound closure rate in second-degree burns (SMD = 3.95, 95% CI: 2.00 to 5.90) was notably sharp compared to third-degree burns (SMD = 6.36, 95% CI: 2.99 to 9.74). However, as might be expected, the therapeutic efficacy in the second and third weeks was better in second-degree burn models (SMD = 3.49, 95% CI: 1.41 to 5.56; SMD = 3.21, 95% CI: 0.99 to 5.43) compared to third-degree burns (SMD = 2.46, 95% CI: 0.31 to 4.60; SMD = 2.83, 95% CI: 1.64 to 4.01), respectively. Additionally, the reduction in I2 suggests that the type of burn model used may be one of the contributing factors to the heterogeneity observed in our results. In addition, the type of scaffolds used also significantly influences therapeutic efficacy. Our results demonstrate that, in the one-week time frame, MSCs combined with biological scaffolds exhibited superior therapeutic efficacy (SMD = 8.83, 95% CI: 0.76 to 16.90) compared to hydrogels (SMD = 4.02, 95% CI: 2.37 to 5.67). However, in the two- and three-week time frames, the therapeutic efficacy of hydrogels (SMD = 3.41, 95% CI: 1.89 to 4.93; SMD = 3.60, 95% CI: 1.31 to 5.89) was greater than that of biological scaffolds (SMD = 2.75, 95% CI: −0.54 to 6.04; SMD = 2.74, 95% CI: 1.56 to 3.91). The absence of a notable reduction in I2 within the scaffold type subgroup can be attributed to the substantial variation in scaffold nature (i.e., polymer-based vs. biological-based scaffolds) and in their constituent components (i.e., natural or synthetic biomaterials). This broad variability in both structural origin and material composition likely contributes to the persistent heterogeneity observed, which may, in turn, affect the robustness and certainty of the derived conclusions.The type of MSCs used significantly influences both heterogeneity and therapeutic efficacy. Our analysis highlights distinct performance patterns across different MSC sources—Umbilical Cord, Bone Marrow, and Adipose—over one-, two-, and three-week time frames. Umbilical Cord-derived MSCs demonstrated the highest therapeutic efficacy in the short term, with a SMD of 6.74 (95% CI: 4.88 to 8.60) at one week. This efficacy remained robust at two weeks (SMD = 6.30, 95% CI: 4.03 to 8.56) but declined slightly by three weeks (SMD = 3.29, 95% CI: 0.64 to 5.94). Similarly, Bone Marrow-derived MSCs exhibited strong therapeutic effects, with an SMD of 6.27 (95% CI: 4.74 to 7.81) at one week, 4.27 (95% CI: 1.73 to 6.81) at two weeks, and 4.42 (95% CI: 1.83 to 7.02) at three weeks. In contrast, Adipose-derived MSCs showed comparatively lower efficacy across all time frames: SMD = 4.16 (95% CI: 1.27 to 7.05) at one week, 1.56 (95% CI: 0.01 to 3.10) at two weeks, and 1.51 (95% CI: −0.28 to 3.30) at three weeks. Furthermore, the observed reduction in I2 suggests that the source of MSCs is a key factor contributing to the heterogeneity in our results. Regarding the source of MSCs, the results demonstrate that human-derived MSCs exhibit significantly higher therapeutic efficacy compared to animal-derived MSCs in the one- and two-week time frames. Specifically, human-derived MSCs showed an SMD of 6.66 (95% CI: 3.38—9.94) at one week and 3.86 (95% CI: 1.20 to 6.52) at two weeks. In contrast, animal-derived MSCs displayed lower efficacy, with an SMD of 3.60 (95% CI: 1.78 to 5.43) at one week and 2.52 (95% CI: 1.02 to 4.02) at two weeks. However, the results in the three-week time frame present a contrasting pattern. Here, animal-derived MSCs demonstrated higher therapeutic efficacy (SMD = 3.54, 95% CI: 0.91 to 6.17) compared to human-derived MSCs (SMD = 2.85, 95% CI: 1.62 to 4.07). Regarding MSC-derived secretomes, our results indicate that secretomes extracted from Adipose-derived MSCs exhibit higher therapeutic efficacy compared to those from Umbilical Cord-derived MSCs in the one- and two-week time frames. Specifically, Adipose-derived secretomes demonstrated an SMD of 3.67 (95% CI: 2.26 to 5.08) at one week and 4.83 (95% CI: − 2.26 to 12.16) at two weeks. In contrast, Umbilical Cord-derived secretomes showed lower efficacy, with an SMD of 2.78 (95% CI: 0.97 to 4.58) at one week and 4.10 (95% CI: 3.20 to 4.99) at two weeks. These findings suggest that Adipose-derived MSC secretomes may offer superior therapeutic benefits in the short to medium term. Furthermore, the results of our meta-regression analysis indicate that no dose–response relationship was observed regarding the number of MSCs administered and their therapeutic efficacy across the three specified time frames (Supplementary Data S2). Also, given the absence of a universally established criterion for classifying sample sizes in preclinical studies, we categorized sample sizes based on the ARRIVE guidelines and the distribution of sample sizes in the included studies [68]. Specifically, we classified sample sizes as small (n < 6, below the minimum recommended threshold), sufficient (n = 6–11), or large (n > 11). Our analysis revealed that, of the 27 included studies, 12 were classified as small, 13 as sufficient, and 2 as large. This distribution suggests that the overall sample sizes are sufficient to ensure the reliability of the meta-analytic results. Furthermore, subgroup analysis based on sample size across all three time frames demonstrated that the greatest therapeutic efficacy was observed in the adequate sample size group. Consequently, smaller sample sizes did not significantly influence the intervention outcomes and were not a determining factor in the strength of the evidence.
Secondary outcomes
Angiogenesis
Our analytical results demonstrate that the synergistic effect of MSCs and scaffolds significantly enhances the expression of CD31, a key marker of angiogenesis (SMD = 6.24, 95% CI: 3.90 to 8.58) (Fig. 5A). This finding underscores the potential of combining MSCs and scaffolds to promote vascularization, which is critical for effective tissue repair and regeneration. However, the I2 values exceeding 50% for both parameters indicate high heterogeneity among the studies included in the analysis.
Fig. 5
Forest plot demonstrating the therapeutic efficacy of MSC-scaffold combinations on: A angiogenesis, B collagen deposition, C inflammatory cytokines, D growth factors
Collagen
The findings from our analysis highlight that the synergistic interaction between and scaffolds significantly enhances collagen deposition at burn wound sites (SMD = 4.97, 95% CI: 2.22 to 7.73) (Fig. 5B). This suggests that the combined application of MSCs and scaffolds plays a pivotal role in promoting tissue regeneration and improving wound healing outcomes. However, the high heterogeneity observed, as indicated by I2 values exceeding 50%, points to substantial variability across the studies included in this analysis.
Inflammatory cytokines
Our analysis reveals that the combined use of MSCs and scaffolds exerts a powerful anti-inflammatory effect, significantly lowering the levels of pro-inflammatory cytokines. Specifically, we observed notable reductions in TNF-α (SMD = − 4.06, 95% CI: − 1.72 to −6.4), IL-6 (SMD = − 6.24, 95% CI: − 2.23 to −10.26), and IL-1 (SMD = − 5.13, 95% CI: − 1.69 to −8.56) (Fig. 5C). These results suggest that the interaction between MSCs and scaffolds plays a critical role in dampening inflammatory pathways, which could have significant implications for therapeutic strategies in inflammatory diseases and tissue repair. However, the high heterogeneity reflected by I2 values exceeding 50% for all three cytokines indicates considerable variability across the studies analyzed.
Growth factors
The results of our analyses indicate that the synergistic effect of MSCs and scaffolds significantly enhances the expression of key growth factors, including TGF-β (SMD = 6.21, 95% CI: − 1.6 to 14.03) and VEGF (SMD = 7.30, 95% CI: 4.85 to 9.75) (Fig. 5D). The observed effect sizes suggest a substantial impact of this combination on promoting growth factor activity. However, the I2 values exceeding 50% for both parameters indicate high heterogeneity among the studies included in the analysis.
Publication bias
To assess publication bias, we employed three widely used methods: the trim and fill method, funnel plot analysis, and Egger’s and Begg’s tests. According to Table 6, for the primary outcome, the asymmetric distribution in the funnel plot and the addition of studies in the trim and fill method across all three time frames suggest the presence of significant publication bias. Furthermore, Egger’s and Begg’s tests yielded p-values < 0.05 at the 1-week and 2-week time points, further supporting the existence of notable publication bias. However, at the 3-week time point, the p-values from Egger’s and Begg’s tests exceeded 0.05, indicating no significant evidence of publication bias during this period. The evaluation of publication bias for secondary outcomes also revealed notable findings. For CD31 expression, inflammatory cytokines, and growth factors, the asymmetric distribution in the funnel plot, the addition of studies in the trim and fill method, and Egger’s test with p-values < 0.05 all indicate significant publication bias. However, Begg’s test yielded p-values of 0.11, 0.12, and 0.76, respectively. The asymmetric funnel plot, the addition of studies in the trim and fill method, and Begg’s test with a p-value < 0.05 suggest significant publication bias. In contrast, Egger’s test showed a p-value of 0.11 (all results are available in Supplementary Data S3).
Table 6 Summary of publication bias and sensitivity analysis resualts for outcomes
Sensitivity analysis
For sensitivity analysis, we employed the one-out remove method to assess the influence of individual studies on the SMD as the effect size metric. According to Table 6, for both the primary outcome and secondary outcomes, the one-out remove method revealed that no outlier studies were identified, indicating that no single study had a significant impact on the overall SMD. This suggests that the results are not disproportionately influenced by any individual study (all results are available in Supplementary Data S4).
The Championships have begun, and the quintessentially British tennis tournament has some very particular codes for attendees to follow. How are spectators expected to behave, and what should they – or should they definitely not – wear?
Afternoon tea, a stiff upper lip and Wimbledon – all so deeply “British” that they’ve become part of the nation’s international brand; the things that seem to make Britain, Britain, and tempt tourists from far and wide to observe them for themselves.
The optics of the annual tennis tournament in south London – the strawberries and cream, the Pimm’s, the ball girls and boys – are all intrinsic to this frightfully British institution. It is a marker of “The Season”, alongside the likes of the Chelsea Flower Show, Henley and Ascot, as well, of course, as being a place where balls are expertly hit by rackets. Equally intrinsic to the Britishness of the tournament are its less tangible cornerstones. Its rules and the etiquette are paramount, both in terms of behaviour and dress – in other words, what to wear, and what falls a little long of the baseline.
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Strawberries and cream are a popular spectator tradition at the Wimbledon Championships (Credit: Getty Images)
So what does the etiquette of Wimbledon entail? For starters, according to the self-styled queen of etiquette, Laura Windsor – who advised on the TV series Bridgerton – don’t call it Wimbledon. “Really, when one refers to Wimbledon, one should say the Championships, as they are the oldest and most prestigious lawn tennis championships in the world.” Another suitable moniker is simply “the tennis”.
Attendees should, she tells the BBC, be behaving “with modesty… That’s our Britishness, isn’t it? We’re prim and proper”. What does this look like? For a start, avoiding many of the kinds of behaviours that are the norm at other sporting events, and instead behaving in ways arguably more in line with theatre-going, keeping things largely hushed, and not cracking out a selfie-stick – they were banned from the tournament in 2015. Although for British etiquette coach and author of Just Good Manners, William Hanson, “anyone who is still using a selfie-stick in 2025 should be monitored closely, anyway”.
Best behaviour
According to Hanson, “while people love the social side of Wimbledon, it is worth remembering that it is a seated, sporting event that comes with its own set of court-side rules.” He goes on: “Be punctual, and get in your seat in good time… respect the fact that you can only come and go during a changeover.” Of course, phones should be on silent. But he also extends his guidance to how best to rally around the players, and officials, on court: “Be sporting with your support, and never cheer errors or mistakes. Respect the silence during play, and save clapping and cheering for between points. Avoid disturbing the players by calling out, and remember the umpire’s decision is final – never heckle or question line calls.”
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The Panama hat has been a style favourite for Wimbledon attendees since the beginning of the 20th Century (Credit: Getty Images)
Beyond that, “keep your yourself to yourself,” he says. “Be aware of those sitting around you, and keep within the space of your seat – even if it is limited, try not to spill over into someone else’s personal space accidentally.” And, in case you were thinking about sneaking in a tuna melt, “avoid disturbing the peace by rustling wrappers or eating messy or smelly food”.
Be comfortable and appropriate for the weather, but avoid anything that is overly casual or better worn at the beach – William Hanson
Most obviously, though, the rules are embodied in what to wear – “the best way to show good behaviour is by dressing appropriately,” says Windsor. While Wimbledon has no strict dress code, smart dress is encouraged, especially on the show courts. Plus certain items, such as ripped jeans, dirty trainers and anything with a political statement, are banned. “Be comfortable and appropriate for the weather,” says Hanson, “but avoid anything that is overly casual or better worn at the beach”.
Unofficial dress code
Unofficially, however, Wimbledon has developed its own set of style rules. Take for proof of these unspoken but still-tangible “rules” the criticism that Meghan, Duchess of Sussex faced from some corners for wearing jeans, alongside a hat and blazer, to watch her friend Serena Williams play in 2019.
For Daniel-Yaw Miller, sports and fashion journalist and founder of the SportsVerse newsletter, “when you think about Wimbledon dressing, everyone has the same kind of thing in mind – either white or beige. You wear a blazer, you can wear a hat.” Guests often favour floral frocks, polka dots and tailoring. By osmosis, a dress code seems to have been landed upon. For Windsor, dressing appropriately means “something that is demure and sophisticated,” such as crisp linen and tailored pieces.
While Hanson points out that “wide-brimmed hats are best avoided as they can get in the way of those sitting behind you,” Panama hats have become something of an official Wimbledon uniform, popular in the audience from the start of the 20th Century.
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Pale-hued, loosely structured tailoring, as worn by Sienna Miller, is a Wimbledon sartorial classic (Credit: Getty Images)
Over the years, a number of high-profile celebrities have offered up glowing examples of how to ace it sartorially in the stands of Centre Court. Zendaya, patron saint of “tenniscore”, schooled Wimbledon-goers last year when she wore Ralph Lauren menswear-inspired looks – crisp white and old school tweed suit jackets, blue-and-white-striped shirts and ties.
Other smashes include Meghan in neat pleats and white shirts, Keira Knightley in a dropped-waist Chanel dress, Tom Cruise, year-on-year, in natty suit after natty suit, Alexa Chung in Aran-knit cardigans and crisp cotton poplin skirts, and Pierce Brosnan in navy linen suits with neat pocket squares. Arguably, however, queen of SW19 dressing is Sienna Miller, thanks to her broderie anglaise dresses and easy-breezy linen suits, bright white Galvan jumpsuits and polka dots. Others have aced it despite not sticking to the script – see Grace Jones in a flight suit for a perfect example, or Idris Elba in a striking Super Eagles shirt.
You go to Wimbledon tennis for a fascinating day out, for an insight into what the past of British society looked like – Daniel Yaw Miller
Catherine, Princess of Wales, who is the royal patron of the All England Lawn Tennis Club, often wears green or purple, the official colours of the tournament since 1909. In recent years she’s attended in a plethora of shades of green, such as a forest green Dolce & Gabbana crepe midi-dress in 2019, and in 2021 an emerald green Emilia Wickstead number – the colour of the grass at the beginning of the tournament, if not the end. Last year, Kate wore a purple Safiyaa dress to present the trophies for the men’s singles.
Sense of tradition
The presence of royalty every year underlines the event’s sense of tradition, and tennis has long been associated with wealth, status and glamour. “Tennis was this social game, rather elite and glamorous,” says Elizabeth Wilson, author of Love Game: A History of Tennis, from Victorian Pastime to Global Phenomenon – its original setting was the country house. “It is, without doubt, a traditionally upper-class sport, and that essence still permeates through a lot of tennis,” says Miller. He likens it to cricket, or Ascot, “where you have to be so respectful, as an outsider coming in, of the rules”.
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The Princess of Wales often wears green or purple – the Championships’ official colours – when attending the tennis (Credit: Getty Images)
Even among tennis tournaments, Wimbledon is extra-proper. “You look at other grand slams, [it] is the polar opposite,” Miller says, mentioning some examples: walk-out music, players dancing as they come out, Kiss Cam, hot dogs for sale and T-shirt guns.
It is, says Miller, “one of the last bastions of old-school British culture in the mainstream”. Its archaic quality is, for some, part of its charm and appeal. “I see Wimbledon as essentially like a museum. You go there for a fascinating day out, for an insight into what the past of British society looked like,” he says.
All this also has its downsides. “From a fan perspective, you’re really made to feel like you’re stepping into a space that is not yours,” says Miller. You need, to a certain extent, “to act like you’ve been there before”.
Shifting codes
Over the years, however, Wimbledon’s codes have been shifting. In lots of ways Wilson thinks “it’s more participatory 1751365187”. In the past, “people have described how the atmosphere was more like a cathedral, there was a rather holy atmosphere – whereas that’s very far from how it is today.”
Hanson agrees. “If we think of the Henman Hill/Murray Mound (and the rest), levels of patriotism and emotion, it is unlike public displays we would have seen in the past.”
Crowds do now whoop and shout, at least between points, and even partake in the occasional Mexican wave, which, according to Wilson, “nobody would have thought of doing in the 1950s”. When Miller went last year, he “could definitely feel that it was a bit more of a modern tennis experience”. He can feel it, he says, “loosening up, just the tiniest bit”.
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Guests who regularly ace the event style-wise, left to right, David Beckham, Alexa Chung and Idris Elba (Credit: Getty Images)
Dress-wise, also, attitudes are softening. Miller sees Idris Elba and David Beckham as shining examples of dressers who know how to be Wimbledon-appropriate without looking stuck in the past. “The look is more ‘fancy barbecue’ than ‘super formal event’”.
For Hanson, points of etiquette prevail. “Etiquette is all about how our actions and behaviours affect or impact other people. We need to be considerate and careful in how we behave to ensure that everyone has a great day watching tennis. Wimbledon thrives on its age-old rituals, and remains one of the key traditional British summer sporting events – hopefully, for this special fortnight, we can all remember our manners, on court and off.”
A new paper reveals caffeine might play a role in slowing down the aging process at a cellular level — by tapping into an ancient cellular energy system. The study of fission yeast — a single-celled organism “surprisingly similar” to human cells — found that caffeine helps cells sustain life.
Caffeine has long been linked to potential health benefits, including reduced risk of age-related diseases. But the researchers say until now, how it works inside our cells, and what exactly are its connections with nutrient and stress responsive gene and protein networks has remained a mystery.
A few years ago, the same research team found that caffeine helps cells live longer by acting on a growth regulator called TOR (“Target of Rapamycin”). TOR is a biological switch that tells cells when to grow, based on how much food and energy is available.
This switch has been controlling energy and stress responses in living things for over 500 million years.
New research suggests caffeine helps cells age slower by activating their internal energy sensor, rather than directly influencing their growth.In the latest study, the scientists discovered caffeine doesn’t act on this growth switch directly. Instead, it works by activating another important system called AMPK, a cellular “fuel gauge” that is evolutionarily conserved in yeast and humans.
“When your cells are low on energy, AMPK kicks in to help them cope,” explains the study’s senior author, Dr. Charalampos Rallis, a reader in Genetics, Genomics and Fundamental Cell Biology at Queen Mary University of London. “And our results show that caffeine helps flip that switch.”
Caffeine’s effects at the cellular level
Interestingly, AMPK is also the target of metformin, a common diabetes drug that’s being studied for its potential to extend human lifespan together with rapamycin, backed by advocates including longevity influencer Bryan Johnson and OpenAI CEO Sam Altman.
Using their yeast model, the researchers showed that caffeine’s effect on AMPK influences how cells grow, repair their DNA, and respond to stress — all of which are tied to aging and disease.
“These findings help explain why caffeine might be beneficial for health and longevity,” says Dr. John-Patrick Alao, the postdoctoral research scientist leading this study.
“And they open up exciting possibilities for future research into how we might trigger these effects more directly — with diet, lifestyle, or new medicines.”
The study findings are published in the journal Microbial Cell.
Previous research has spotlighted caffeine’s effects on human life quality, particularly for infants. A Rutgers Health study revealed that it may protect babies by preventing dangerous drops in oxygen that can cause death. Sudden Unexpected Infant Death is the leading cause of infant deaths between one and 12 months old.
ITV has commissioned a serial killer thriller series called The Dark from ex-BBC drama controller Ben Stephenson’s Poison Pen.
The series is adapted by Matt Hartley based on GR Halliday’s debut novel From the Shadows. Poison Pen was launched by Stephenson under ITV Studios‘ production banner in 2023.
When the body of a young man is found eerily staged in the idyllic Scottish wilderness, detective Monica Kennedy fears this is just the beginning of a terrifying campaign that will strike the heart of a rural community. “As paranoia rises, suspicions and secrets are forced into the light, and the locals start to realise that there is a serial killer hidden amongst them,” a plot synopsis reads.
“With her experience of dealing with the darkest of humanity, Monica quickly becomes entangled in a heightened game of cat and mouse with a cunning killer,” it continued.
“But when her own history creeps up on her, she begins to lose trust in her own judgement. Will it be her own actions that are the biggest risk to herself and her family?”
ITV’s director of drama Polly Hill greenlit the six-part show. “The Dark is a compelling new drama which introduces the fearless DI Monica Kennedy, who has to try and catch a serial killer in this really chilling Scottish story,” said Hill. “Matt’s adaptation is brilliantly gripping and I am delighted to be adapting this novel with him and the team at Poison Pen.”
Stephenson added: “The Dark is a gripping hide behind the sofa thriller with unguessable twists and a central character whose mysterious past gives her an extraordinary insight into a twisted killer as well as an overpowering empathy for those left in the killer’s wake.”
Stephenson, Preethi Mavahalli, Luke Woellhaf and Fern McCauley will executive produce alongside Matt Hartley. Matt Brown will be producing the series, which also has Lena Rae and Nessah Muthy writing and Gilles Bannier as lead director and executive producer.
The Dark will commence filming in and around Glasgow during 2025 and is produced in association with ITV Studios, who will also distribute the series internationally.
Nintendo has announced an imminent “pricing update” for the original Switch in Canada, leaving fans north of the border expecting the aging console to increase in price — more than eight years after it launched.
An advisory warning issued by Nintendo of Canada states that the changes will be fully detailed later today, July 1, as prices are publicly adjusted on the official Nintendo Canada online store.
Explaining the changes, Nintendo’s note simply states the move is “based on market conditions,” without going into further detail.
Nintendo has said that the newly-launched Switch 2 will not be impacted by the impending price adjustment, though all models of the original Switch (the OG Switch, Switch Lite and Switch OLED) will be impacted.
Physical and digital Switch games and accessories will also see price changes, Nintendo continued — something which of course will impact Switch 2 owners, as most games and many peripherals are cross-compatible.
The sweeping changes will also hit Nintendo Switch Online memberships, the company stated, as well as amiibo figurines. If you’re in Canada and need to top-up your online subscription before prices change, now might be a good time.
Canada, like many other countries, has been caught up in the ongoing financial uncertainty provoked by the introduction of US tariffs. Prior to the Switch 2 launch, Nintendo paused the rollout of pre-orders in the US and Canada as it gauged the potential impact of tariffs, before announcing price rises on some Switch 2 peripherals.
Nintendo president Shuntaro Furukawa previously suggested the impact of tariffs could impact the company’s profits by “tens of billions of yen,” though cautioned that increasing the Switch 2’s price to compensate would likely “decrease demand.”
Fans have suggested that these price rises, aimed at the original Switch, and on Nintendo Online Memberships, are likely Nintendo’s workaround — without attracting the headlines of raising the Switch 2’s price so soon after launch.
Tom Phillips is IGN’s News Editor. You can reach Tom at tom_phillips@ign.com or find him on Bluesky @tomphillipseg.bsky.social
A new study demonstrates that sarcopenia-related muscle decline associated with aging can be modeled within a relatively short period in space, paving the way to study potential treatments quicker and more effectively.
To understand the changes of muscle in microgravity, researchers at the University of Florida (US) engineered skeletal muscle microtissues from donor biopsies and launched them to the International Space Station aboard SpaceX CRS-25.
Their findings may inform therapies for sarcopenia, which is common with aging and affects up to 50% of people aged 80 and older, according to estimates.
“Using electrical pulses to trigger real-time muscle contractions in space, we can simulate exercise and observe how it helps protect against rapid muscle weakening in microgravity,” explains Siobhan Malany, one of the lead researchers.
“This technology advancement offers insight into how we might preserve muscle health during long-duration space missions and ultimately, how to combat age-related muscle loss here on Earth.”
Spaceflight-induced muscle weakness offers a rapid model for studying age-related sarcopenia, which typically develops over decades on Earth.Apart from lifestyle changes, there is no current clinical treatment for sarcopenia. It can lead to disability and injuries from falls and is associated with a lower quality of life and an increased mortality.
Muscles in space
Space flight comes with the absence of gravity and limited strain on muscles, which causes weakness, a prominent feature of sarcopenia, within a short period of time. This offers a “time lapse view” on age-related atrophy-associated changes in the muscle, highlight the researchers.
“This relatively short window of time in space provides a microgravity model for muscular aging and opens opportunities for studying sarcopenia, which normally takes decades to develop in patients on Earth,” notes the research team.
The microtissues were taken from young, active donors, and aged, sedentary donors and cultured in an automated mini lab. Besides regular feeding and monitoring of cultures, the lab also performed electrical stimulation to simulate exercise.
On Earth, the contraction strength of microtissues from young, active individuals was almost twice as much as the strength of tissues from older, sedentary individuals. After only two weeks in space, muscle strength tended to decline in the young tissues and was now more comparable to the strength of old tissues.
A similar trend was observed in muscle protein content, which was higher in young microtissues on Earth compared to old microtissues but decreased in microgravity to levels measured in old tissues. Further, space flight changed gene expression — particularly in the younger microtissues — and disturbed cellular processes related to normal muscle function.
Interestingly, the scientists found that electrical stimulation could mitigate these changes in gene expression “to some extent.”
Their findings were published in Stem Cell Reports.
In other aging-focused space research, David Beckham’s IM8 supplement brand, co-founded with Prenetics, sent specially designed 3D organoids — miniature, simplified versions of human tissues — into space to study accelerated aging. The researchers also leveraged microgravity’s unique environment that speeds up this process.
In a successful operation carried out by security forces in the Teri Mangal area of Kurram District, two terrorists, namely Hafeez-ur-Rehman and Wajid Gul, were killed, security forces said on Tuesday.
Both were wanted in connection with the shooting of schoolteachers and had bounties of Rs5 million each on their heads, according to security officials.
Security sources said the two men were part of a group responsible for the killing of five teachers in Teri Mangal in 2023. During the exchange of fire, a local resident was injured with a gunshot wound to the leg.
On May 2023, six school teachers were killed in attacks across Upper Kurram. Officials said it began with the drive-by shooting of a teacher, Muhammad Sharif, on Solozan Road.
Read: Operation launched to bring peace to Kurram
Shortly after, armed men stormed into Teri Mangal High School, entered the staffroom, and opened fire on teachers, who were supervising examinations. Six teachers and one staff member were killed on the spot.
The deceased educators were identified as Mir Hussain, Jawad Hussain, Naveed Hussain, Jawad Ali, Muhammad Ali and Ali Hussain, all members of the Turi Bangash tribe.
Security agencies confirmed that five suspects linked to the 2023 incident were already on the police and law enforcement’s wanted list. Two of them were killed in today’s operation while the search for the remaining three fugitives continues.
The Khyber-Pakhtunkhwa government had announced bounties for Hafeez-ur-Rehman, Wajid Gul and three others in July 2024.
Security forces said further measures are being taken to maintain peace in the area and that operations will continue until the complete elimination of terrorists.
Recent violence in the region
In a separate incident last week, a roadside bomb blast in the Sharamkhel area of Pewar, also in Upper Kurram, killed four people and injured four others, police said.
According to District Police Officer Malik Habib, the explosion occurred as the victims were heading to a nearby hill to collect firewood. Two horses were also killed in the blast.
The deceased were identified as Nawab Ali, Amir Hussain, Hidayat Hussain and Manzar Ali. Three of the injured were later shifted to Peshawar for specialised care, according to Parachinar DHQ Hospital officials.
Violence in Kurram has surged in recent months. In December, more than 100 people were killed in tribal clashes that left parts of the region isolated for weeks.
