Author: admin

The successful trial provides a path to future immunotherapies, assessing advanced biomaterial-based cancer vaccines in combination with checkpoint blockade inhibitors

By Benjamin Boettner 

(BOSTON) — The first-in-human phase I clinical trial assessing the feasibility and safety of WDVAX, an immunostimulatory biomaterial-based cancer vaccine, in a cohort of 21 patients with stage 4 metastatic melanoma, was concluded with positive outcomes that encourage future vaccine developments and trials to test them in combination with immune checkpoint inhibitor therapies.

Initiated in 2013 by the Wyss Institute at Harvard University and the Dana-Farber Cancer Institute (DFCI), and led by F. Stephen Hodi, Jr., M.D., Director of DFCI’s Melanoma Center and Professor of Medicine at Harvard Medical School (HMS), the trial demonstrated that a precisely engineered biomaterial-based multi-component system is feasible to be consistently fabricated and applied to patients. The significant immune-activating functions of the monotherapy correlated with 43% of the patients exhibiting stable disease.

The novel vaccine concept originated in the laboratory of Wyss Founding Core Faculty member David Mooney, Ph.D. Mooney, together with his group at the Wyss Institute and the Harvard John A. Paulson School of Engineering and Applied Sciences (SEAS), advanced the novel immunotherapy solution through a series of foundational studies. In addition to developing the capabilities needed to fabricate the biomaterial vaccines, the Mooney group, in collaboration with that of Glenn Dranoff, M.D., who at the time was a Wyss Associate Faculty member and co-leader of DFCI’s Cancer Vaccine Center, had carried out extensive preclinical studies to demonstrate the approach’s efficacy.

The team demonstrated that the vaccines can induce the regression of multiple types of tumors in animal models and, in addition, provided prophylactic protection. These foundational and translational efforts led to the first-in-human WDVAX clinical trial assessing this new type of cancer vaccine, whose results were recently published in Cancer Immunology Research.

The vaccination concept holds that a porous biomaterial scaffold made from a widely used biodegradable medical polymer and infused with bioactive molecules can function as a training ground for the immune system. By infusing the scaffold with the cytokine molecule GM-CSF, dendritic cells (DC) – central orchestrators of immune responses – are recruited into the porous scaffold interior, where they are activated by additionally infused CpG oligonucleotides that function as an adjuvant. To prime DCs specifically against cancer cells, the WDVAX trial team loaded the scaffold with inactivated tumor antigens derived from tumors of the trial participants. The Mooney and Dranoff teams’ preclinical studies had shown that, upon their activation, the reprogrammed DCs migrate from the scaffold to nearby lymph nodes, where they activate T cells that engage in a coordinated attack on tumor cells.

The development and study of the personalized cancer vaccine was funded by the Wyss Institute, DFCI, and the National Institutes of Health, and the crucial technologies invented for its realization are owned or co-owned by Harvard University, DFCI, and the University of Michigan.

From feasibility to immune activation

To demonstrate the clinical feasibility of WDVAX therapy in a dose-escalation study of melanoma patients, the trial’s goal was to show that a fabrication process could be put into place that was able to produce sufficient WDVAX vaccines within 28 days following the resection of patients’ tumors to allow for multiple consecutive treatments of the same patients with personalized vaccines. Using the Mooney lab’s proprietary recipe, team members at DFCI’s Cell Manipulation Core Facility loaded inactivated tumor antigens contained in lysed tumor samples into the biomaterial scaffolds and successfully manufactured WDVAX vaccines in a beneficial time frame for all patients in the 15-patient expansion cohort. They also succeeded in producing sufficient vaccine for seven out of eight additional trial participants. The study thus established a clinically applicable vaccine manufacturing process and showed that vaccine fabrication per se likely won’t be a bottleneck for evaluating similar types of biomaterials-based vaccines in future trials.

To introduce WDVAX vaccines into patients’ bodies, surgeons on the team simply had to make a small incision in the upper arm or thighs, implant the manufactured vaccines under the skin, and suture the wound. The patients were then closely monitored for signs of immune reactions and often exhibited a significant local response at the implant site.

Next, to evaluate the feasibility of conducting WDVAX clinical trials, the team’s other goals were to assess the overall survival of the vaccine recipients and to analyze their immune responses. After receiving their tumor-specific WDVAX vaccines, none of the patients developed treatment-related adverse events with life-threatening consequences, and, importantly, 43% of all patients in the entire cohort had stable disease. The researchers compared patients’ tumor samples before and after vaccination and found that WDAX treatment indeed induced T cells and other types of immune cells in the tumor microenvironment. Specifically, the numbers of CD4+ T cells that normally coordinate immune attacks within tumors were found to be variably increased, cytotoxic CD8+ T cells with tumor cell-killing potential had variably infiltrated the tumors, and myeloid cells of the immune system also were present at elevated numbers.

At the same time, they also found that T cells that entered tumors upon WDVAX vaccination upregulated so-called checkpoint proteins. Checkpoint proteins generally act as “brakes” that prevent T cells from attacking tumor cells. Hodi, Mooney, and the team therefore speculate that taking a two-pronged approach in future clinical trials could be promising. By combining WDVAX-like vaccines with widely used checkpoint inhibitor therapy, a tumor-specific T cell response could be launched in tumor environments in which the immune system’s ability to fight the tumors would be additionally enhanced. Such a coordinated immunotherapy could advance treatment success to potentially enable the regression of tumors, as seen by Mooney’s group for monotherapy with WDVAX vaccines alone in preclinical animal models carrying aggressive melanoma tumors.

We are confident that integrating newer insights in next-generation approaches and combining those with other forms of immunotherapy, including checkpoint inhibitor therapy, can result in new breakthroughs in the treatment of patients suffering from different types of cancer.

Supercharging the next wave of biomaterial vaccine

“Since the WDVAX trial was started, our group and other groups have made a number of important advances in the biomaterial cancer vaccine space. Next-generation injectable [not implantable] scaffolds have emerged that, among other favorable characteristics, have the ability to further shape immune responses. Also, the technological capabilities of identifying patient-specific tumor antigens with immune-activating potential are constantly evolving,” said Mooney. “We are confident that integrating these newer insights in next-generation approaches and combining those with other forms of immunotherapy, including checkpoint inhibitor therapy, can result in new breakthroughs in the treatment of patients suffering from different types of cancer.” Mooney is also the Robert P. Pinkas Family Professor of Bioengineering at SEAS.

“The promising results of this world-first trial evaluating a personalized, biomaterial-based cancer vaccine in patients with an aggressive cancer are a testament to the Wyss Institute’s capability of driving potentially life-saving medical developments from their initial inception, through research and preclinical stages, all the way to the patients that need them,” said Wyss Institute Founding Director Donald Ingber, M.D., Ph.D. “Dave Mooney’s team and their collaborators at the DFCI created an important translational path that will super-charge the next wave of such innovative cancer vaccines at the Wyss and at other cancer centers around the world.” Ingber is also the Judah Folkman Professor of Vascular Biology at Harvard Medical School and Boston Children’s Hospital, and the Hansjörg Wyss Professor of Biologically Inspired Engineering at SEAS.

Other authors on the study were Anita Giobbie-Hurder, Kwasi Adu-Berchie, Srin Ranasinghe, Ana Lako, Mariano Severgnini, Emily Thrash, Jason Weirather, Joanna Baginska, Michael Manos, Edward Doherty, Alexander Stafford, Heather Daley, Jerome Ritz, Patrick Ott, Kathleen Pfaff, Scott Rodig, and Charles Yoon.