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Asrar A. Alahmadi, MBBS, is heading a first-in-human, open-label, phase 1 clinical trial (NCT06801002) investigating JBZ-001, an orally bioavailable small-molecule inhibitor of dihydroorotate dehydrogenase (DHODH) for the treatment of patients with advanced solid tumors and non-Hodgkin lymphoma.

Developed by Jabez Biosciences, JBZ-001 targets the de novo pyrimidine biosynthesis pathway by inhibiting DHODH, the rate-limiting enzyme in this pathway. Preclinical studies demonstrated JBZ-001’s broad antitumor activity across a range of cell lines and patient-derived xenograft models, particularly in aggressive cancers such as lymphoma, leukemia, and small cell lung cancer. The drug showed higher potency and lower toxicity compared with other agents in its class, suggesting its potential as both monotherapy and in combination with chemotherapy.

The phase 1 study now follows a standard 3+3 dose escalation design to determine the optimal biological dose (OBD), rather than the maximum tolerated dose. Pharmacokinetic and pharmacodynamic assessments include a novel blood-based biomarker to monitor DHODH activity in real time. Once OBD is reached, expansion cohorts will explore efficacy signals in specific tumor types.

The trial is currently enrolling patients, with future plans for combination studies and phase 2 trials in promising indications such as small cell lung cancer and hematologic malignancies, according to Alahmadi, assistant professor at The Ohio State University and lead principal investigator at The Ohio State University Comprehensive Cancer Center-James Cancer Hospital & Solove Research Institute.

In an interview with Targeted Oncology^TM, Alahmadi discussed the trial in progress and key goals of the study.

Targeted Oncology^TM: Can you discuss the mechanism of action of JBZ-001?

Alahmadi: The drug is a small molecule that inhibits a very critical enzyme involved in DNA and RNA synthesis, which is essential in rapidly dividing cells. That’s essentially what cancer is—unchecked, rapid cell proliferation. The molecule blocks DHODH, which is part of the pyrimidine biosynthesis pathway. This pathway is vital for cell survival during rapid division. The inhibition shows promising results against aggressive cancers like lymphoma and leukemia, and we’ve also seen potential in some relapsed solid malignancies like small cell lung cancer.

What preclinical data supported the initiation of this phase 1 study?

We observed efficacy in cell lines, patient-derived xenograft models, and through large-scale screening across multiple malignancies. We demonstrated broad activity, which we believe is due to the drug’s mechanism. It targets cancer cell metabolism, giving it a wide scope of action.

What is the study design, including the planned dose-escalation scheme and patient eligibility?

This is a first-in-human study. We’re testing the drug as a monotherapy using a standard 3+3 dose-escalation design. We’re collecting pharmacokinetic and pharmacodynamic data, including a blood-based marker—DHO/DHODH level—which correlates with enzyme activity. Our aim is to identify the optimal biological dose, rather than just the maximum tolerated dose. Once we reach OBD, we will proceed with expansion cohorts, especially in areas where we observe potential efficacy.

What are the key goals of the study? What are you hoping to learn about the tolerability of the drug?

We want to assess safety and tolerability, of course. Preclinical data suggest this agent may be more potent and less toxic than other DHODH inhibitors. We’re hoping this translates into the clinic—more efficacy with lower toxicity. Looking ahead, we aim to test it in combination with chemotherapy to enhance treatment outcomes in solid tumors. We also want to determine the optimal biological dose that balances efficacy and safety, which aligns with the FDA’s Project Optimus initiative.

Assuming the safety and preliminary efficacy are favorable, what are the potential next steps in the clinical development of this agent?

The next step would be testing in combination with chemotherapy. Also, if we observe efficacy signals in specific cancer types, we’ll open expansion cohorts and move into phase 2 trials. For example, we’re already seeing encouraging signs in small cell lung cancer. There’s evidence from other groups, including large CRISPR-based screens, suggesting that small cell lung cancer heavily depends on the pyrimidine biosynthesis pathway—making this an attractive target.

What are the key takeaways about this study and what should oncologists know about recruitment or the drug itself?

So far, our preclinical data suggest this drug may be more potent than other DHODH inhibitors. We’re currently enrolling patients in the dose expansion phase. I think that’s a key point.

I think we can always do better in our fight against cancer. Over the years, clinical trials have brought promising new treatments to market—treatments that genuinely improve patients’ lives, like targeted therapies and immunotherapies. With this development, we hope not only to show this drug is effective, but also to identify biomarkers of response. That way, we can deliver the right drug to the right patient at the right time.

REFERENCE:

Alahmadi A, Bennett C, Biglione S, et al. Abstract CT199: An open-label phase 1 study to investigate JBZ001 in adults with advanced solid tumors and non-Hodjkin lymphoma (JBZ001, trial in progress). Cancer Res. 2025;85 (8_Supplement_2): CT199. doi:10.1158/1538-7445.AM2025-CT199

Introduction

Antimicrobial resistance has become a global concern, posing significant challenges in the treatment of infectious diseases.¹ Beta-lactam antibiotics, including penicillins, cephalosporins, carbapenems, and aztreonam, are commonly prescribed for bacterial infections.² However, the widespread use of third generation cephalosporins to combat Gram-negative bacterial infections has contributed to the development of resistance against beta-lactam antibiotics.³ Consequently, this has led to the emergence of organisms producing Extended-Spectrum Beta-Lactamases (ESBLs) and carbapenemase, further exacerbating the issue.⁴ In 2024, the World Health Organization (WHO) classified carbapenem-resistant Pseudomonas aeruginosa, carbapenem-resistant Acinetobacter baumannii, and Carbapenem-resistant Enterobacterales (CRE) as “critical” and “high” priority pathogens.⁵

The spread of antibiotic-resistant organisms in health care facilities and in the community is a worrying epidemiological problem that could be influenced by the rate of faecal carriage of antibiotic-resistant organisms.⁶ The Enterobacterales, regardless of whether they are community or hospital-acquired, primarily originate from the digestive tract.⁷ This area is a hub for the exchange of resistance genes and antibiotic treatments, which can lead to the overgrowth of resistant bacteria.⁸ When antibiotic resistant isolates colonize the intestinal compartment, the risk of infection due to these bacteria significantly increases.⁹ Additionally, the intestinal compartment may act as a source for ESBL- and carbapenemase-producing resistance genes, which can spread to other Enterobacterales through horizontal transmission.¹⁰ Hence, being aware of the frequency of patients carrying resistant bacteria in their digestive tracts can serve as a means of forecasting the extent to which these bacteria may cause infections and spread among people.^11–13In the past decade, there has been a global rise in reported cases of hospital-acquired infections caused by Gram-negative bacilli (GNB) that are resistant to carbapenem antibiotics.^14–17 Hospitalized patients face a double burden as they not only have to deal with their health problems but also with a severe infection of this scale. Hence, this critical situation is a matter of concern for all healthcare systems.^18,19

In Africa, ESBL-PE (ESBL Producing Enterobacterales) carriage in the gastrointestinal tract ranges from 10% to 50%, highlighting the severity of the situation.²⁰ In Benin, a West African country, government has formally validated its National Action Plan (NAP); however, there are ongoing efforts to implement these measures, and as of now, the NAP has not yet been included on the WHO NAP website.²¹ More resources are required to develop laboratory testing capacity for the accurate detection of antibiotic-resistant bacteria throughout the country.²² While several studies have reported systematic data about AMR prevalence in general or focused on specific sample types, there is a lack of data regarding fecal carriage of antibiotic-resistant bacteria.^23–28 Recent studies in Benin have reported high rates of multidrug-resistant pathogens and revealed the first detection of a plasmid-encoded New-Delhi metallo-beta-lactamase-1 (NDM-1) producing Acinetobacter baumannii from surgical site infections within hospitals.^29,30 Prior to this, a study reported the first occurrence of hospital-originated Pseudomonas aeruginosa producing carbapenemase VIM-2.³¹ Additionally, there is an increased risk of infection associated with care,^24,32 and wound contamination by hospital bacteria.^24,33 Therefore, this study aims to determine the one-day prevalence of faecal carriage of ESBL and carbapenemase producing Gram-negative bacilli, along with associated risk factors and to characterize the resistant isolates.

Methods

Study Design and Sampling

This cross-sectional study, conducted in December 2022, focused on fecal samples from post-surgery patients (accidents and injury-related cases) in tertiary hospitals in Benin. A consecutive sampling approach was employed, where stool/rectal samples were collected from every consenting patient who had been hospitalized for more than 48 hours, until the target sample size of 30 was reached in each hospital. This 48-hour cutoff was used to distinguish hospital-acquired from community-acquired colonization. Four hospitals (Central Hospital and University of Mother and Child Lagoon of Cotonou, Departmental Hospital Center of Zou and the Hills, Departmental Hospital Center of Borgou-Alibori, and Hospital Saint Jean de Dieu of Tanguiéta) participated in the study, with patients with digestive pathologies excluded. The sample size was determined based on the bed capacity of the surgery wards in each hospital, ranging from 39 to 47 beds. All samples were collected on the same day, following strict aseptic protocols. A questionnaire was administered to gather sociodemographic information, clinical data, and medical history. The study received prospective ethical approval from the Ethics and Research Committee of the Institute of Applied Biomedical Sciences (CER-ISBA) under approval number 154. Written informed consent was obtained from each participant or their parent/guardian before participation, with a clear explanation of the study’s objectives. All research activities were conducted in accordance with the Declaration of Helsinki.

Species Identification, Antibiotic Susceptibility Testing, ESBL and Carbapenemase Detection

Fecal samples were screened for betalactam and carbapenem resistance using CHROMID^® ESBL and CHROMID^® CARBA SMART agar media (Bio-Rad, USA). After 24 hours of incubation at 37°C, distinct colonies were selected based on their characteristic color on the selective media. Pure cultures were obtained using again CHROMID^® ESBL and CHROMID^® CARBA SMART agar. Gram-negative bacilli, excluding Stenotrophomonas maltophilia, were identified using Matrix-Assisted Laser Desorption Ionization–Time of Flight (MALDI-TOF) mass spectrometry (Bruker Daltonics, Bremen, Germany). Antibiotic susceptibility testing was performed with inoculum from a subculture on Mueller–Hinton agar using the disc diffusion method on Mueller–Hinton agar, following recommendations from the European Committee on Antimicrobial Susceptibility Testing.³⁴ Various antibiotic discs manufactured by Bio-Rad were used for the testing. ESBL production was confirmed using the double disk synergy technique. For Enterobacterales, discs (Bio-Rad, United States) containing cephalosporins including cefotaxime 30 µg, ceftazidime 30µg, cefepime 30µg were applied next to clavulanic acid disc following recommendations from EUCAST.³⁵ For Pseudomonas spp, imipenem double-disc synergy test (DDS-IPM) with discs containing ceftazidime 30µg, cefepime 30µg and a disc with imipenem 10µg as the ESBL inhibitor was used.³⁶ Acinetobacter spp were tested for ESBL production by placing discs containing piperacillin (30 μg) and a combination of piperacilline + tazobactam (30–6µg) on agar plates.³⁷ Resistance to one of carbapenems tested was used as an indicator for the continuation of tests for detecting carbapenemase production. On these strains, the carbapenemase production test was carried out using the rapid diagnostic kit NG-TEST CARBA 5 (Hardy Diagnostics, California, United States) capable of detecting KPC, OXA-48-like, VIM, IMP, and NDM carbapenemases. Reference strain of Escherichia coli ATCC 25922 was used as quality control for the antibiotic susceptibility test.

Detection of Antibiotic Resistance Genes

Bacterial DNA was extracted from strains isolated from ESBL agar using boiling method. Multiplex PCR was performed to identify isolates carrying betalactamase encoding genes,³⁸ carbapenemase encoding genes³⁹ and PMQR genes.⁴⁰ Each reaction was prepared in a total volume of 25 µL using OneTaq^® Quick-Load^® 2X Master Mix with Standard Buffer (Biolabs, South Africa) and following the manufacturer instructions. The DNA fragments underwent electrophoresis in a 2% agarose gel, and the results were interpreted by comparing the migration of the fragments to marker sizes. The reference strain Escherichia coli ATCC 25922 was used as a negative control, while clinical strains producing ESBL and carbapenemase served as positive controls for the PCR assays.

Statistical Analysis

Data entry was performed using Excel 2019, and statistical analysis was conducted using Stata SE 11 software. Univariate analysis was initially conducted, selecting variables with a p-value less than 0.05 as potentially associated with ESBL and carbapenemase producing bacteria carriage. Results were reported as odds ratio (OR) with corresponding 95% confidence intervals, using an interpretation threshold of α = 0.05. The chi-square test was also performed to determine the degree of association between factors and independent variables.

Results

Factors Associated with the Carriage of ESBL and Carbapenemase Producing Gram Negative Bacteria

Table 1 described the factors associated with the carriage of ESBL Producing Gram negative bacteria. Out of the 120 patients enrolled in the study, 46 were identified as carriers of ESBL-producing bacteria, resulting in a prevalence of ESBL carriage at 38.33%. The study of the association of variables shows a high degree of association (p < 0.05) with ESBL carriage for the factors: independent walking and use of a wheelchair (Table 1).

Table 1 Factors Associated with the Carriage of ESBL Producing Bacteria

Table 2 described the factors associated with the carriage of carbapenem-resistant bacteria. Out of the 120 patients enrolled in the study, 59 were identified as carriers of carbapenem-resistant bacteria, resulting in a prevalence of 49.16%. Analysis of the association between variables revealed no statistically significant associations (p > 0.05) among the factors studied (Table 2).

Table 2 Factors Associated with the Carriage of Carbapenem-Resistant Bacteria

Species Identification and Percentage of Antibiotic Resistance

Table 3 shows the results of the identification of the isolated bacterial species. A total of 92 strains were isolated using ESBL agar. Among the bacterial species isolated from ESBL agar, Escherichia coli (44.56%) was the most frequently isolated, followed by Pseudomonas aeruginosa (26.08%) and Acinetobacter baumannii (10.87%). Notably, all Enterobacter cloacae strains (100%), most Escherichia coli strains (82.93%), and Klebsiella pneumoniae strains (80%) were found to be ESBL producers. A total of 83 strains were isolated using carba agar. The identification of isolates from carba agar showed a predominance of the species Escherichia coli (44.45%) followed by the species Klebsiella pneumoniae (16.06%) and Acinetobacter baumanii (13.58%). A total of 64 (77.10%) isolates were found to be resistant at least to one of the tested carbapenem antibiotics. Klebsiella pneumoniae (92.30%) and Escherichia coli (75%) were the most represented species.

Table 3 Distribution of Isolates According to the Culture Media

The resistance percentage of the isolates from ESBL agar is presented in Table 4. Notably, high rates of resistance were observed for ofloxacin (ranging from 97.11% to 100%), amoxicillin + clavulanic acid (ranging from 79.41% to 100%), and cefotaxime (ranging from 50% to 100%) among the tested antibiotics. Most Enterobacter cloacae, Escherichia coli, Klebsiella pneumoniae strains demonstrated multidrug resistance. On the other hand, Pseudomonas aeruginosa strains exhibited susceptibility to most of the tested antibiotics, except for cefsulodin. Furthermore, the lowest levels of resistance were observed with antibiotics such as imipenem, ertapenem, and temocillin (Table 4).

Table 4 Percentage of Antibiotic Resistance of Isolates Recovered from ESBL Screening Agar

The study of the resistance profile of Enterobacterales isolates from carba agar was presented in Table 5. The results showed that all isolates (100%) were resistant to amoxicillin and piperacillin. Similarly, a higher rate of resistance was observed to carbapenem, ranging from 87.50% to 100%. Various resistance rates were observed for other antibiotics.

Table 5 Percentage of Antibiotic Resistance of Enterobacterales Isolates Recovered from Carba Screening Agar

The study of the resistance profile of Pseudomonas spp was presented in Table 6. The results showed that isolate of Pseudomonas putida was resistant to all tested antibiotics excepted amikacin. Similarly, 80% (4/5) of Pseudomonas aeruginosa isolates exhibited resistance toward all tested antibiotics but some isolates (3/5) were susceptible to amikacin and aztreonam.

Table 6 Percentage of Antibiotic Resistance of Pseudomonas Spp Isolates Recovered from Carba Screening Agar

The study of the antibiotic resistance profile of Acinetobacter spp was presented in Table 7. The results showed that isolate of Acinetobacter nosocomialis was resistant to all tested antibiotics. Higher resistance rate (>80%) was also observed for Acinetobacter baumannii but 41.66% were susceptible to Amikacin.

Table 7 Percentage of Antibiotic Resistance of Acinetobacter Spp Isolates Recovered from Carba Screening Agar

Distribution of Carbapenemase Types

A total of 64 isolates resistant at least to one of the tested carbapenem antibiotics were selected for NG-TEST CARBA 5 (KPC, OXA-48-like, VIM, IMP, and NDM). All isolates selected were found to be carbapenemase producers. NDM (43.08%) was the most detected type of carbapenemase followed by strains for which carbapenemase type was not detected (30.77%) (Table 8).

Table 8 Distribution of Carbapenemase Types Among Species Isolated by Phenotypic Test

Table 9 Distribution of Detected Carbapenemase Gene Among Species Isolated

Antibiotic Resistance Genes Detection

Tables 9 and 10 present the distribution of resistance genes in carbapenemase and ESBL producing isolates. bla_NDM (54.68%) was the most detected carbapenemase genes. Among the ESBL-producing isolates, the presence of five ESBL genes was identified and presented in Table 10. The most detected genes were bla_CTXM-1 and bla_CTXM-15, accounting for 91.49% of the isolates. Additionally, the bla_TEM (36.17%) and bla_OXA-1 (29.78%) were found in the isolates. Quinolone resistance genes were prevalent among the isolates, with qnrb present in 70.21% of the isolates, followed by qnrs in 63.82% of the isolates, and aac(6′)-ib-cr in 53.19% of the isolates. Among the different species, Escherichia coli exhibited a high prevalence (97.05%) of the bla_CTXM-1 gene, while Enterobacter cloacae had a prevalence of 80% for the same gene. Electrophoresis gel images are shown in supplementary data (Figures S1–S6).

Table 10 Distribution of Resistance Genes in ESBL Producing Isolates

Discussion

The emergence and global spread of extended-spectrum β-lactamase (ESBL) and carbapenemase producing Enterobacterales pose a significant threat to public health.²⁰ The objective of this study was to determine the one-day prevalence of faecal carriage of ESBL and carbapenemase producing Gram-negative bacilli, along with associated risk factors, and to characterize the resistant isolates.

In this study, a culture-dependent approach was used to detect ESBL-producing- and carbapenem-resistant Gram-negative bacteria. The results of this study revealed a significant prevalence of carriage of ESBL-producing bacteria (38.33%), indicating a potential risk for nosocomial infections and the dissemination of antimicrobial resistance genes in the community. Lower rates of ESBL-PE carriage prevalence were reported in other African countries such as Gabon (11.8% to 16.7%), Cameroon (15% to 18%), Central African Republic (19.3%), and Nigeria (20.9%).^41–44 In Madagascar, the prevalence of ESBL-PE among individuals was found to be 10.1%.⁴⁵ On the other hand, a higher prevalence of ESBL-PE was reported in Burkina Faso.⁴⁶ Disparities in prevalence rates among countries and the increasing prevalence of ESBL-producing bacteria in Africa have been highlighted.⁴⁷ However, our findings do not align with their estimation that the overall rate of ESBL-PE in clinical samples was below 15%. Concerning carbapenem resistance detection, out of the 120 samples that were collected, a total of 81 strains were isolated. As 59 were identified as carriers of carbapenem-resistant bacteria, the prevalence of carbapenem resistance carriage is therefore 49.16%. A study conducted in Egypt revealed that 62.7% of Enterobacterales isolates were resistant to carbapenems, which is consistent with our findings.48 Similarly, high rates of carbapenem resistance were observed in South Africa (68%),⁴⁹ and Sudan (83%).⁵⁰ Respectively, 28.6% and 35% rates of resistance were observed in Uganda and Tanzania.^51,52 The prevalence of ESBL-PE values among individuals in different African countries varies significantly not only between countries, as underlined by the authors, but also between cities, sites (such as rural versus urban areas, and hospitals versus communities), and over different years. This variability should be highlighted even if the provided data remain indicative. The unrestricted use of antibiotics, which is prevalent among most of the population in low- and middle-income countries (LMICs) in Africa especially in Benin, is likely to lead to an increase in carbapenem resistance in the region.⁵³ In this study, the samples were collected within one day (24 hours), and it is not possible to estimate whether these results show an intermittent or regular situation in the study area. Sampling at different times of the year could give a more reliable profile of the situation.

In this study, no significant association was found between these risk factors and carriage of ESBL-producing bacteria. Similar investigations focusing on carbapenemase-producing isolates did not yield comparable results.^54,55 The study of the association of variables shows a high degree of association (p < 0.05) for the factors sex, independent walking, and use of a wheelchair. Patients who use wheelchairs or have limited mobility spend generally more time in healthcare facilities, potentially increasing their exposure to resistant isolates. Also, young patient age has been regarded as a risk factor for Carbapenem-resistant Enterobacterales (CRE) infection.⁵⁰ Length of hospital stay, sex, age, presence of immunosuppression, independent walking, bedridden patient, diabetic patient, presence of sign of infection, antibiotic treatment, history of hospitalization in the last 6 months, antibiotic treatment last 6 months, patient with faecal/urinary incontinence were the other risk factors examined in this survey. Neither of these risk factors was significantly associated with carbapenemase producing strains carriage. Numerous studies have shown interest in assessing the risk factors associated to carbapenemase producing isolates carriage but none of them have found similar results.^54,55

In total, 51.08% of the isolated strains were found to be ESBL producers. The identification of ESBL producing bacteria species revealed a predominance of Escherichia coli (82.93%), followed by Enterobacter cloacae (100%; n = 5) and Klebsiella pneumoniae (80%; n = 4). Notably, Enterobacter cloacae, Escherichia coli, and Klebsiella pneumoniae strains exhibited high proportions (100%, 82.93%, and 80% respectively) of ESBL production. Similar species predominance was observed in recent studies.^44,56 Other studies conducted in Cameroon and China have reported a higher diversity of ESBL-PE species, including Enterobacter spp. and Citrobacter spp.^57,58 Nevertheless, E. coli consistently emerges as the most identified species during colonization by ESBL-PE. It is worth noting that these bacteria, which pass through the human intestine, have the potential to acquire resistance through horizontal gene transfer.⁴⁶ The identification of bacteria with resistance to carbapenems showed a predominance of the species Escherichia coli (44.45%) followed by the species Klebsiella pneumoniae (16.06%) and Acinetobacter baumanii (13.58%). Same isolates were reported in various studies.^48–52 Patients with carbapenem-resistant Enterobacterales often face treatment failure, prolonged hospital stay, high expenses and a high possibility of death.⁵⁹

The antibiotic resistance phenotype results of isolates from ESBL agar showed high rate of resistance for ofloxacin (ranging from 97.11% to 100%), amoxicillin + clavulanic acid (ranging from 79.41% to 100%), and cefotaxime (ranging from 50% to 100%) among the tested antibiotics. Multidrug resistance was common among Enterobacter cloacae, Escherichia coli and Klebsiella pneumoniae strains. Similar observations were made,⁴⁴ indicating significant resistance not only to β-lactam antibiotics due to ESBL production but also to other antibiotic families, including quinolones. In this study, the carbapenems (imipenem and ertapenem) showed the highest effectiveness against ESBL-producing Gram-negative bacteria (GNBs). These carbapenems are considered expensive last-resort drugs in the region but have demonstrated favorable activity against GNBs that produce this enzyme.^60–62 This finding is consistent with a report which indicates the widespread distribution of ESBL producers among Enterobacterales.⁵⁰ The study of antibiotic susceptibility patterns of carbapenem-resistant isolates revealed that the majority of isolates were resistant to all tested antibiotics, including carbapenems. Distribution of carba types according to species isolated and ESBL production showed that 52.31% of isolates were ESBL producers, but none non-Enterobacterales was found to be ESBL producing. All isolates selected were found to be carbapenemase producers. NDM (43.08%) was the most detected type of carbapenemase followed by other types (30.77%) using the aforementioned phenotypic method. The NDM enzyme was frequently encountered among Enterobacterales as one of the most common carbapenemases, and it was also observed in A. baumannii and P. aeruginosa isolates.^63,64 Most of non-Enterobacterales were found to produce other types of carbapenemase. This is consistent with a study reporting that carbapenemase producers are becoming highly distributed among Enterobacterales.⁵⁰ Non-Enterobacterales including Pseudomonas spp employ mainly porin expression reduction and increased chromosomal cephalosporinase activity against carbapenems.⁶⁵

The study extensively examined the presence of ESBL genes among the isolates. The most frequently detected ESBL genes were bla_CTXM-1 and bla_CTXM-15 (91.49%), followed by bla_TEM (36.17%). These genes are associated with resistance to a broad range of β-lactam antibiotics. Similar findings have been reported in other studies conducted in Cameroon (96% of isolates), Indonesia (94.5%), and Tunisia (91%), indicating the global dominance of CTX-M-type ESBLs.^57,66,67 The study also investigated the presence of other resistance genes associated with quinolone and carbapenem resistance. The isolates exhibited the presence of quinolone resistance genes, such as qnrb (70.21%), qnrs (63.82%), and aac(6′)-ib-cr (53.19%), indicating the potential for reduced efficacy of fluoroquinolone antibiotics in treating infections caused by ESBL-producing bacteria. This result supports the concept co-expression due to the isolates harboring several antibiotic resistance genes. Furthermore, the presence of qnr genes and aac(6′)-ib-cr alongside ESBL-encoding genes further strengthens the possibility of co-selection.⁶⁸ These findings confirm the multidrug-resistant nature of ESBL-PE, which severely limits available therapeutic options and contributes to the widespread use of carbapenems. The detection of carbapenemase-encoding genes in a study is vital for understanding and addressing the growing problem of antibiotic resistance. It provides critical information for infection control, treatment decisions, and the development of strategies to preserve the effectiveness of antibiotics in the face of rising resistance. In this study, bla_NDM (54.68%) was the most detected carbapenemase genes mainly in Klebsiella pneumoniae (91.66%), Enterobacter cloacae (77.77%) and Pseudomonas aeruginosa (75%). Our results are consistent with others studies in Sudan, Senegal, and South Africa.^49,50,59 This confirms that bla_NDM could demonstrate a capacity for rapid dissemination.⁶⁹ The plasmids that carry carbapenemase genes, such as bla_NDM, exhibit significant diversity and can harbor numerous additional resistance genes, including ESBL-alleles. The presence of multiple resistance genes in certain isolates, as observed in this study, indicates the presence of multidrug-resistant pathogens. These pathogens are responsible for treatment failures, outbreaks of infections, and increased treatment costs.

The primary aim was to determine the prevalence of ESBL and carbapenemase-producing bacteria at a single time point in hospitalized post-surgical patients, providing baseline data for future longitudinal studies on acquisition dynamics. The one-day design and >48-hour hospitalization criterion limit differentiation between community- and hospital-acquired colonization. Without admission screening or follow-up, acquisition timing and transmission pathways cannot be confirmed. Also, Limited clinical and hospital data restrict analysis of risk factors and transmission routes. Therefore, conclusions about nosocomial spread should be interpreted cautiously. The absence of whole genome sequencing prevents detailed epidemiological and transmission analysis. Future studies with longitudinal sampling, comprehensive data, and genomic tools are needed to clarify acquisition and guide interventions.

Conclusion

This study reports a high one-day prevalence of fecal carriage of extended-spectrum β-lactamase (ESBL) and carbapenemase-producing Gram-negative bacteria among post-surgical patients hospitalized for over 48 hours in Benin. The predominance of Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii, combined with high resistance to β-lactams and fluoroquinolones, underscores the clinical and public health significance of these pathogens. Genotypic analysis revealed frequent detection of bla_CTX-M and bla_NDM, along with co-resistance genes, indicating the circulation of multidrug-resistant organisms in the hospital setting. While this cross-sectional study cannot confirm nosocomial acquisition, it provides important baseline data to inform future surveillance and infection control efforts in Beninese hospitals.

Abbreviations

CTX-M: Cefotaxime-Munich; ESBL: Extended-Spectrum β-lactamase; ESBL-PE: ESBL Producing Enterobacterales; GNB: Gram Negative Bacilli; MBL: Metallo-betalactamase; NDM: New Delhi Metallo-betalactamase.

Data Sharing Statement

All data generated and/or analyzed during the current study are included in this published article. The datasets used and/or analyzed during this study are also available from the corresponding author on reasonable request.

Ethical Approval

The study proposal was reviewed and approved by the Ethics and Research Committee of the Institute of Applied Biomedical Sciences (CER-ISBA) under number 154. Written informed consent was obtained from each patient or their parent/guardian before participation, accompanied by a concise explanation of the study’s objective. The research work (sampling from hospitalized patients, sample processing and data analysis) in our study was conducted in accordance with the Declaration of Helsinki.

Acknowledgments

The authors are very grateful to Alida Oussou, Donald Bokossa, Lydie Comlan, Adonias Houefonde and Yedia Djohoun for their great help during the implementation of this study. They are also very grateful to all the staff of the hospitals involved in the study and their willing to support any kind of interventions for a better care of patients. They thank the patients who accepted to participate in this study.

Author Contributions

All authors made a significant contribution to the work reported, whether that is in the conception, study design, execution, acquisition of data, analysis and interpretation, or in all these areas; took part in drafting, revising or critically reviewing the article; gave final approval of the version to be published; have agreed on the journal to which the article has been submitted; and agree to be accountable for all aspects of the work.

Funding

This research received no external funding.

Disclosure

The authors declare no conflict of interest.

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Introduction

Obstructive sleep apnea (OSA) is characterized by recurrent sleep disturbances leading to intermittent hypoxemia, hypercapnia, and disrupted sleep architecture.^1–3 An epidemiological study indicates that the estimated prevalence of OSA with an apnea-hypopnea index (AHI) ≥5 events/hour ranges from 17% to 26% in men and 9% to 28% in women, with rates of 4% to 9% in middle-aged men and 1% to 2% in middle-aged women.⁴ According to the diagnostic criteria of AHI ≥5 events/hour, it is estimated that there are 176 million OSA patients in China, with 66 million being moderate to severe cases.^5,6 Studies have demonstrated that OSA can lead to nocturnal hypoxia, resulting in sympathetic activation, inflammation, oxidative stress, metabolic disorders, and endothelial dysfunction, potentially leading to multi-organ and multi-system damage such as cardiovascular diseases, diabetes, arrhythmias, cerebrovascular accidents, and cognitive impairments.^3,7–9 Untreated severe OSA patients have a mortality rate 3.8 times higher than that of the general population. The probability of stroke occurrence in the OSA population is 4.33 times higher than in the control group, with a mortality rate 1.98 times higher than the control group.^10,11

The primary assessment indicators for OSA currently include total AHI, lowest blood oxygen saturation, longest apnea duration, and longest hypoventilation period. Clinical experience demonstrates that relying solely on these indicators does not provide a comprehensive reflection of the severity of the patient’s condition. The longest apnea duration, longest hypoventilation period, and lowest blood oxygen saturation measurements offer insights at specific time points, while the AHI value merely indicates the frequency of events throughout the night without capturing the duration and extent of hypoxia.^12,13 This lack of effective evaluation indicators leads to inadequate understanding and assessment of OSA conditions, resulting in insufficient patient awareness of the condition’s severity and subsequently lower rates of consultation, diagnosis, and treatment for OSA.¹⁴ Economical and efficient screening for patients with OSA, coupled with a thorough evaluation of their condition, can enhance patient awareness and facilitate early intervention, thereby generating significant societal benefits and enhancing the overall quality of life for the population. The China-PAR model has been developed to validate 10-year risk predictions for atherosclerotic cardiovascular disease (ASCVD) among the Chinese population. This model incorporates various factors, including gender, age, waist circumference, total cholesterol, high-density lipoprotein cholesterol, treated or untreated systolic blood pressure, current smoking status (yes/no), diabetes mellitus (yes/no), and family history of ASCVD.¹⁵ It emphasizes anthropometric measurements and risk factors that are commonly observed in patients with OSA. Therefore, the China-PAR model is particularly suitable for assessing the 10-year ASCVD risk in patients with OSA in our study.

The investigation into cardiovascular risks among patients with OSA during episodes of hypoxia is gaining increasing significance. Nocturnal hypoxia can induce oxidative stress, inflammation, sympathetic activation, endothelial dysfunction, metabolic dysregulation, and pro-thrombotic effects and drive ASCVD and myocardial ischemia.^16–18 Previous studies have shown that recurrent hypoxia in OSA is related to pulmonary arterial hypertension, and vascular and microvascular diseases in patients.^1,2,10,11,19 The importance of new body indices and hypoxic burden metrics in the assessment of cardiovascular disease risk in OSA patients cannot be overlooked. For example, Yeşildağ M et al reported that the body roundness index, a novel body metric for defining and predicting cardiovascular risk in patients with OSA, was significantly associated with the oxygen desaturation index, a critical metric that measures hypoxic burden and links OSA to cardiovascular conditions.²⁰ However, the association between hypoxia events and patient prognosis assessment remains unclear. The hypoxic burden is calculated by dividing the sum of the product of the desaturation area associated with each pause or hypopnea event by the total sleep time. It can be automatically calculated by advanced sleep monitoring data analysis software, reflecting the intensity and duration of hypoxic events.²¹ The percentage of time with blood oxygen saturation below 90% (T90%) reflects the total duration of hypoxia throughout the night.²² However, due to the lack of comprehensive prognostic analysis, there are currently no standardized grading criteria for the hypoxic burden and T90%. The role of hypoxic burden and T90% in assessing the condition and prognosis of OSA patients remains to be further explored.

Polysomnography (PSG) is considered the primary diagnostic tool for identifying OSA. However, the equipment associated with PSG is costly, requires a specific installation environment, involves complex operation, demands high proficiency from medical personnel, necessitates completion in medical facilities during the examination process, and often leads to a suboptimal patient experience. These factors make it challenging to use PSG efficiently in screening for OSA among high-risk populations. Studies have shown that portable sleep monitors demonstrate a high level of agreement with PSG outcomes and offer reliable diagnostic capabilities for OSA, prompting an increased recognition of their clinical utility.^23,24

Here, by using portable sleep apnea monitors, we investigated the relationship between oxygen desaturation burden and T90% and myocardial ischemia and cardiovascular risk, promoting the establishment of a more comprehensive OSA assessment system. Our research has the potential to serve as a foundation for OSA patients to receive enhanced diagnostic and therapeutic interventions, ultimately leading to improved patient outcomes.

Materials and Methods

Ethics Statement

This study was carried out in accordance with the Declaration of Helsinki. All procedures were performed in compliance with relevant laws and institutional guidelines and have been approved by the Ethics Committee of Shenzhen Qian Hai She Kou Free Trade Zone Hospital (No.: 2021K-057). Informed content was obtained from all patients.

Study Participants

This prospective observational study was conducted at a single tertiary center in Southern China from January 2022 to March 2024. Hospitalized patients who underwent portable sleep apnea screening tests after being highly suspected of having OSA were screened. Only patients who were subsequently diagnosed with OSA were included. Inclusion criteria: 1) patients with daytime sleepiness, lack of energy after waking up, fatigue, or insomnia. 2) patients who woke up due to suffocation, wheezing, or choking at night. 3) patients with habitual snoring or breathing interruptions. 4) patients with dizziness. 5) patients with hypertension or poor response to hypertension treatment. 6) patients with emotional disorders. Exclusion criteria: 1) patients with known coronary artery diseases; 2) patients with a history of stroke; 3) patients with arrhythmias or those who used antiarrhythmic medications; 4) patients with cardiomyopathy; 5) patients with valvular heart disease; 6) patients who had electrolyte imbalances; 7) patients with severe kidney insufficiency.

Diagnostic Criteria for OSA

The diagnosis and initial assessment of OSA were based on the International Classification of Sleep Disorders.²⁵ The diagnostic threshold for OSA was defined by an AHI of ≥ 5 events per hour, where AHI values of 5–15 were classified as mild, 15–30 as moderate, ≥ 30 as severe, and ≥ 60 as extremely severe. The severity of nocturnal hypoxia was classified into five categories: normal, mild, moderate, severe, and very severe, determined by the lowest oxygen saturation levels: ≥90%, 85%-89%, 80%-84%, and <80%.

Sleep Monitoring

Participants were instructed to wear the portable sleep monitor, Phillip Alice Night One (Respironics, CA, USA), for overnight sleep monitoring. This device has been validated against PSG and has demonstrated a high level of agreement with PSG outcomes.^23,24 Sleep duration was assessed by considering the position, nasal airflow patterns, and self-reported sleep onset time of patients. Data was collected using Sleepware G3 4.0.1.0 (Respironics, CA, USA), manually reviewed and interpreted. Then, the hypoxic burden and other assessment parameters were automatically calculated. The recorded indicators included: AHI, hypoxic burden, supine hypoxic burden, non-supine hypoxic burden, lowest blood oxygen saturation, percentage of total sleep time with apneas, percentage of total sleep time with hypopneas, and percentage of total sleep time with blood oxygen saturation below 90% (T90%), 85% (T85%), 80% (T80%).

Electrocardiogram (ECG) Examinations

All patients underwent routine ECG examinations. The ECGs were assessed and reported by physicians at or above the attending level. The ECG indicators for evaluating myocardial ischemia included: 1) ST segment depression or elevation (ST segment depression in two or more adjacent leads, with limb leads ≥ 0.05 mV or precordial leads ≥ 0.1 mV); 2) T wave flattening or inversion); 3) ST-T changes; 4) positive PTFV1 (P-wave terminal force in electrocardiogram lead V1).

Assessment of the 10-Year Atherosclerotic Cardiovascular Disease (ASCVD) Risk

The process for assessing the 10-year ASCVD risk using the China-PAR model involved two steps.¹⁵ In Step 1, high-risk individuals were directly identified based on specific criteria, including 1) diabetic patients aged ≥40 years; 2) LDL ≥4.9 mmol/L or total cholesterol ≥7.2 mmol/L; 3) chronic kidney disease at stage 3/4. Due to the potential impact of kidney insufficiency on electrolytes and electrocardiographic waveforms, patients with chronic kidney insufficiency were excluded from this study. In Step 2, the remaining patients were subjected to the 10-year ASCVD risk assessment using the China-PAR model. Patients were categorized as either having low or moderate-to-high risk of ASCVD.

Data Collection

The baseline clinical data of patients were collected, including medical history, personal history, medication history, height, weight, abdominal circumference, and current residence. The data on biochemical indicators (such as total cholesterol, high-density lipoprotein, low-density lipoprotein (LDL), and triglycerides) were also collected.

Sample Size Calculation and Power Analysis

The sample size was determined using the standard formula. Assuming a moderate effect size corresponding to an expected odds ratio (OR) of approximately 3–10 and an estimated risk of ASCVD around 50% (α=0.05), we calculated a necessary sample size of approximately 200 to 350 participants for adequate statistical power.

Power analysis was conducted to validate the statistical power of our sample size, using the following formula: Power = 1 – β, where β indicates Type II error rate (commonly set at 0.2 for 80% power).

Statistical Analysis

Statistical analyses were conducted using SPSS 19.0 software. The normally distributed continuous data are expressed as mean ± standard deviation, and comparisons between two groups were performed using independent samples t-test. The non-normally distributed continuous data are expressed as median (interquartile range), and group comparisons were conducted using the Kruskal–Wallis H-test. Categorical data are presented as counts (percentages) and were analyzed using the χ²-test. A P-value of < 0.05 was considered statistically significant.

Binary logistic regression was used to analyze the relationships between the factors in the model and the risk for moderate-to-high 10-year ASCVD and myocardial ischemia. The factors analyzed in the model included AHI, hypoxic burden quartiles, percentage of total sleep time with apneas, percentage of total sleep time with hypopneas, percentage of total sleep time with apneas and hypopneas, T90%, T85%, T80%, and grade of lowest blood oxygen saturation. Hypoxic burden, percentage of total sleep time with hypopneas, percentage of total sleep time with apneas, and percentage of total sleep time with apneas and hypopneas were categorized into four quartiles. Confounders including gender, age, waist circumstance, total cholesterol, high-density lipoprotein, treated or untreated systolic blood pressure, current smoking (yes/no), diabetes mellitus (yes/no), and family history of ASCVD were adjusted. The OR and 95% confidence interval (CI) values were recorded. The receiver operating characteristic (ROC) curve was plotted to assess the diagnostic values of the hypoxic burden and T90%. The area under the curve (AUC), P-value, the optimal cut-off value, sensitivity, specificity, and Youden’s index were calculated. The maximum Youden’s index corresponds to the optimal balance of sensitivity and specificity, thereby determining the optimal cutoff value.

Results

Baseline Clinical Data of Patients

This study included a total of 311 hospitalized patients diagnosed with OSA from January 2022 to March 2024. Basic clinical information of the patients is summarized in Table 1. The median age of the included patients was 53 years, with 75.6% being male and 24.4% female. Among the patients, 51.4% (160 cases) showed ECG changes of myocardial ischemia. Additionally, 55.3% (172 cases) had moderate-to-high 10-year ASCVD risk. As shown in Table 2, the first to third hypoxic burden quartiles for patients with low 10-year ASCVD risk were 44.2 min/h, 73.8 min/h, and 123.4 min/h, respectively. In contrast, the corresponding quartiles for patients with moderate to high risk were 83.0 min/h, 176.1 min/h, and 373.2 min/h. For patients who exhibited negative changes on the ECG indicating myocardial ischemia, the first to third quartiles were 42.8 min/h, 69.8 min/h, and 129.4 min/h, compared to 95.3 min/h, 179.3 min/h, and 384.3 min/h for those with positive changes.

Table 1 Clinical Information of Patients

Table 2 The Quartile Distribution of Hypoxic Burden Among Patients with Moderate-to-High ASCVD Risk and Myocardial Ischemia

Comparison of Sleep Monitoring Characteristics in Patients with Different ASCVD Risk Levels

Kruskal–Wallis H-test indicated significant differences in sex, age, and medical history (Table 3). Independent samples t-test revealed no significant difference between the low 10-year ASCVD risk group and the moderate-to-high 10-year ASCVD risk group with the lowest blood oxygen saturation (Table 3). However, significant differences were observed in AHI, hypoxic burden, supine hypoxic burden, non-supine hypoxic burden, percentage of total sleep time with apnea, percentage of total sleep time with hypopnea, percentage of total sleep time with apnea and hypopnea, T90%, T85%, and T80% (Table 3).

Table 3 Comparison of Sleep Monitoring Parameters in OSA Patients with Low versus Moderate-to-High 10-year ASCVD Risk

Analysis of Baseline Clinical Data and Sleep Monitoring Measures Concerning Myocardial Ischemia Changes

As shown in Table 4, the Kruskal–Wallis H-test indicated that there were no significant differences in sex and age between patients with and without myocardial ischemia changes on ECG. The independent samples t-test showed no significant differences in body mass index, total cholesterol, high-density lipoprotein, or LDL between the two groups. In terms of sleep monitoring indicators, there were no significant differences between the two groups in the percentage of total sleep time with hypopneas, T85%, T80%, or lowest blood oxygen saturation. However, significant differences were found in AHI, hypoxic burden, supine hypoxic burden, non-supine hypoxic burden, percentage of total sleep time with apneas, percentage of total sleep time with apneas and hypopneas, and T90% between the two groups.

Table 4 Comparison of Clinical Data and Sleep Monitoring Indicators Between Patients with and without Myocardial Ischemia Changes on ECG

Analysis of Factors Influencing 10-Year ASCVD Risk and Myocardial Ischemia in OSA Patients

The binary logistic regression analysis was then performed to evaluate factors influencing 10-year ASCVD risk and myocardial ischemia in OSA patients. The factors included in the model were hypoxic burden grading, AHI grading, percentage of total sleep time with hypopnea, percentage of total sleep time with apnea, percentage of total sleep time with apnea and hypopnea, T90%, T85%, and T80%. Our results showed that hypoxic burden and T90% were significantly associated with the high 10-year ASCVD risk in OSA patients. For the index of hypoxic burden grade, the P-value was 0.024, while hypoxic burden between 75%-100% quartile showed statistically significant risk compared with patients in 0%-25% quartile (Q1), with an OR of 13.5 and a 95% CI of 1.684 to 109.051 (Table 5). For T90%, the P-value was 0.022, with an OR of 1.085 and a 95% CI of 1.012 to 1.164 (Table 5). Similarly, hypoxic burden grade and T90% were significantly associated with myocardial ischemia in patients with OSA. For the index of hypoxic burden grade, the P-value was 0.044. Both Q2, Q3, and Q4 exhibited statistically significant risks compared to patients in the 0%-25% quartile (Q1), with P-values of 0.011, 0.008, and 0.048, respectively. The OR was 3.241 (95% CI: 1.306–8.043), 4.497 (95% CI: 1.494–13.538), and 4.850 (95% CI: 1.017–23.139) for Q2, Q3, and Q4, respectively. For T90%, the P-value was 0.019, with an OR of 1.059 and a 95% CI of 1.009 to 1.110 (Table 6). Additionally, the power analysis showed that with the expected ORs of the hypoxic burden ranging from 3 to 11, our study provided a power greater than 90%. For T90%, even though the ORs were between 1.059 and 1.085, as a continuous variable, the analysis indicated that changes in this index across its range still resulted in sufficient power, estimated between 85% and 92%, confirming the reliability of our findings. These results revealed a significant association between T90% and hypoxic burden with high 10-year ASCVD risk and myocardial ischemia.

Table 5 Analysis of Risk Factors for High 10-Year ASCVD Risk in OSA Patients

Table 6 Analysis of Risk Factors for Myocardial Ischemia in OSA Patients

ROC Curve analysis of the Diagnostic Value of Hypoxic Burden for Moderate-to-High 10-Year ASCVD Risk and Myocardial Ischemia in OSA Patients

To determine the value of the hypoxic burden and T90% in diagnosing 10-year ASCVD risk and myocardial ischemia, the ROC curve was plotted. The results showed that the hypoxic burden and T90% (Figure 1) had diagnostic values for moderate-to-high 10-year ASCVD risk (Table 7). For hypoxic burden, the area under the curve was 0.747, with P<0.0001. The Youden’s index was used to determine the cut-off value of the hypoxic burden, which was 125.8 (%min/h). The corresponding sensitivity and specificity were 62.2% and 77.0%, respectively. For T90%, the AUC was 0.754. The cut-off value of T90% was 3.05%, with the corresponding sensitivity and specificity of 65.1% and 71.9%, respectively.

Table 7 Diagnostic Value of Hypoxic Burden and T90% for Moderate-to-High 10-Year ASCVD Risk in OSA Patients

Figure 1 ROC curve analysis of hypoxic burden and T90% in diagnosing moderate-to-high 10-year ASCVD risk in OSA patients.

Similarly, the hypoxic burden and T90% (Figure 2) also showed diagnostic values for myocardial ischemia (Table 8) in OSA patients. The AUC for the hypoxic burden and T90% was 0.769 and 0.741, respectively. The cut-off value for the hypoxic burden was 112.6 (%min/h), with a sensitivity of 70.6% and a specificity of 70.2%. For T90%, the cut-off value was 4.20%, the sensitivity was 60.6%, and the specificity was 77.5%.

Table 8 Diagnostic Value of Hypoxic Burden and T90% for Myocardial Ischemia in OSA Patients

Figure 2 ROC curve analysis of hypoxic burden and T90% in diagnosing myocardial ischemia in OSA patients.

Discussion

The results of this study revealed a strong association between hypoxic burden and T90% and both moderate-to-high 10-year ASCVD risk and myocardial ischemia in OSA patients. These indicators, reflecting the intensity and duration of hypoxic events during sleep, provided valuable insights into the severity of OSA and its cardiovascular implications. In fact, the cutoff values derived from this study are highly consistent with clinically observed OSA severity and have significant implications for evaluating patients with a high AHI, short-duration events, and low overall nocturnal hypoxia. These metrics provide essential clinical guidance in determining whether patients require more aggressive treatments, such as continuous positive airway pressure therapy. Rather than relying solely on AHI and minimum oxygen saturation for severity assessment, clinicians are more inclined to consider measures of hypoxia duration and intensity. The hypoxic burden index and T90% more accurately meet the requirements of clinicians. Notably, the traditional evaluation indicators, such as AHI and the lowest blood oxygen saturation, did not have such associations, highlighting the limitations of current OSA severity assessment indicators. The higher hypoxic burden and T90% underscore the risk of intensity and duration of hypoxic events in 10-year ASCVD risk and myocardial ischemia for OSA patients. Additionally, the findings emphasize the importance of incorporating these advanced indicators into routine clinical practice for a more comprehensive evaluation of cardiovascular risk in OSA patients. These indicators can help clinicians identify high-risk patients early and develop tailored treatment strategies.

The association of cardiovascular risk with hypoxic events in OSA patients is receiving increasing attention. Previous studies^1,2,10,11,19 have indicated that recurrent hypoxia in OSA is related to pulmonary hypertension and vascular and microvascular diseases. The oxygen desaturation index, a critical metric that measures hypoxic burden, links OSA to cardiovascular conditions in the context of hypoxia. It is correlated with the body roundness index, a novel body metric used to define and predict cardiovascular risk in patients with OSA.²⁰ However, the relationship between hypoxic events and patient prognosis still requires further investigation. The hypoxic burden, which reflects the intensity and duration of hypoxic events, can be automatically calculated by advanced sleep monitoring data analysis software. The T90% reflects the total time spent with blood oxygen saturation below 90% throughout the night, providing insight into the overall severity of OSA during the night.²² This study indicates that the hypoxic burden and T90% were significantly related to both the 10-year ASCVD risk and myocardial ischemia. As the increase of the hypoxic burden, the risk of moderate-to-high 10-year ASCVD and myocardial ischemia also elevated. Although previous studies have investigated the relationship between T90% and hypoxic burden-related indicators and cardiovascular disease, there is currently a lack of cut-off values for these indicators for risk prediction, leading to an inability to classify the severity of patients and resulting in different research conclusions.^26,27 This study established cut-off values for T90% and hypoxic burden in predicting the risk of myocardial ischemia and cardiovascular disease. These results may provide a basis for defining the severity and offer important guidance for the clinical treatment of myocardial ischemia and cardiovascular complications in patients with OSA.

PSG is limited in clinical use due to its high cost and complex operation. Portable sleep monitors, however, show high consistency with PSG in diagnosing OSA.²³ This study utilized portable sleep monitoring and included 311 OSA patients of different ages and genders from southern China. Given that the portable sleep monitor we used in this study is validated for accuracy and reliability against traditional PSG, it enables clinicians to perform comprehensive assessments in a variety of settings, including home care. This flexibility allows for the timely identification of at-risk patients and could facilitate earlier interventions, which are critical for aggressive management strategies in OSA. Furthermore, the China-PAR model, which is tailored for the Chinese population, was used to assess the 10-year ASCVD risk, allowing for an evaluation of long-term cardiovascular risk. Additionally, ECG was used to assess current myocardial ischemia, providing a comprehensive assessment of both long-term cardiovascular risk and current myocardial ischemia risk in OSA patients.

This study has some limitations. For example, the exclusion of patients with electrolyte disturbances and kidney insufficiency might have underestimated the impact of oxygen desaturation on myocardial ischemia and cardiovascular risk assessment. Another limitation of this study is the modest AUC values and small ORs of the hypoxic burden and T90%, which may limit their clinical utility. Further research is warranted to confirm our findings and explore the applicability of these indices in diverse clinical settings.

Conclusion

This study demonstrates that hypoxic burden and T90% are valuable indicators for assessing cardiovascular risk and myocardial ischemia in OSA patients. These metrics have diagnostic potential for identifying myocardial ischemia and cardiovascular diseases, with implications for clinical practice. While these findings highlight the diagnostic potential of nocturnal hypoxia monitoring for cardiovascular complications, our study is single-center research; further validation in larger, prospective cohorts is necessary before widespread clinical adoption. Additionally, the impact of hypoxia on both short-term and long-term cardiovascular risk events in OSA patients warrants further research and attention.

Abbreviations

OSA, Obstructive sleep apnea; ASCVD, Atherosclerotic cardiovascular disease; PSG, Polysomnography; ECG, Electrocardiogram.

Data Sharing Statement

The datasets generated and/or analyzed during the current study are available from the corresponding author on reasonable request.

Author Contributions

Wenmei Zeng: Conceptualization, Methodology, Resources, Software, Funding acquisition, Writing – original draft, Writing – review & editing. Sulong Wu: Conceptualization, Methodology, Resources, Writing – original draft, Writing – review & editing. Zhuofan Liu: Conceptualization, Investigation, Resources, Writing – review & editing. Long Yuan: Conceptualization, Investigation, Resources, Writing – review & editing. Bilin Chen: Software, Methodology, Resources, Writing – review & editing. Yan Rong: Conceptualization, Funding acquisition, Formal analysis, Data curation, Writing – review & editing, Project administration. All authors gave final approval of the version to be published; have agreed on the journal to which the article has been submitted; and agree to be accountable for all aspects of the work.

Funding

This work was supported by the Technical R & D and Creative Design Project sub-funds, Nanshan, Shenzhen, China [grant numbers NS2021002; NS2022013].

Disclosure

The authors report no conflicts of interest in this work.

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