Fluorescent immunohistochemistry was performed as previously described (Mommersteeg et al., 2010). Embryos were dissected and fixed overnight in 4% paraformaldehyde (ChemCruz, SC-281692). Following a brief wash in PBS (Gibco, 18912–014),…
Endothelial Slit2 guides the Robo1-positive sympathetic innervation during heart development
