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  • Inpatient versus outpatient management of community-acquired acute skin and soft tissue infections. Clinical outcomes and factors associated with eligibility for early discharge | BMC Infectious Diseases

    Inpatient versus outpatient management of community-acquired acute skin and soft tissue infections. Clinical outcomes and factors associated with eligibility for early discharge | BMC Infectious Diseases

  • Bouza E, Burillo A, Muñoz P. How to manage skin and soft-tissue infections in the emergency department. Curr Opin Infect Dis. 2023;36(2):81–8.

    Article 
    PubMed 

    Google Scholar 

  • Grossi AP, Ruggieri A, Vecchio A, Del Comandini A, Corio L, Calisti F, et al. Skin infections in Europe: a retrospective study of incidence, patient characteristics and practice patterns. Int J Antimicrob Agents. 2022;60(3):106637.

    Article 
    PubMed 

    Google Scholar 

  • Peterson RA, Polgreen LA, Cavanaugh JE, Polgreen PM. Increasing Incidence, Cost, and seasonality in patients hospitalized for cellulitis. Open Forum Infect Dis. 2017 Feb 8;4(1)ofx008.

  • Kaye KS, Petty LA, Shorr AF, Zilberberg MD. Current Epidemiology, Etiology, and burden of acute skin infections in the united States. Clin Infect Dis. 2019;68(Supplement3):S193–9.

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Falcone M, Tiseo G. Skin and soft tissue infections in the elderly. Curr Opin Infect Dis. 2023;36(2):102–8.

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Adly M, Woo TE, Traboulsi D, Klassen D, Hardin J. Understanding dermatologic concerns among persons experiencing homelessness: a scoping review and discussion for improved delivery of care. J Cutan Med Surg. 2021;25(6):616–26.

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Dellsperger S, Kramer S, Stoller M, Burger A, Geissbühler E, Amsler I, et al. Early switch from intravenous to oral antibiotics in skin and soft tissue infections: an algorithm-based prospective multicenter pilot trial. Open Forum Infect Dis. 2022;9(7):ofac197.

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Mohammed SA, Roberts JA, Cotta MO, Rogers B, Pollard J, Assefa GM, et al. Safety and efficacy of outpatient parenteral antimicrobial therapy: a systematic review and meta-analysis of randomized clinical trials. Int J Antimicrob Agents. 2024;64(2):107263.

    Article 
    PubMed 

    Google Scholar 

  • Tran TT, Gomez Villegas S, Aitken SL, Butler-Wu SM, Soriano A, Werth BJ et al. New perspectives on antimicrobial agents: Long-Acting lipoglycopeptides. Antimicrob Agents Chemother. 2022 Jun 21;66(6)(6):e0261420.

  • Hazen A, Pizzicato L, Hom J, Johnson C, Viner KM. Association between discharges against medical advice and readmission in patients treated for drug injection-related skin and soft tissue infections. J Subst Abuse Treat. 2021;126:108465.

    Article 
    PubMed 

    Google Scholar 

  • Carratalà J, Rosón B, Fernández-Sabé N, Shaw E, del Rio O, Rivera A, et al. Factors associated with complications and mortality in adult patients hospitalized for infectious cellulitis. Eur J Clin Microbiol Infect Dis. 2003;22(3):151–7.

    Article 
    PubMed 

    Google Scholar 

  • Macía-Rodríguez C, Alende-Castro V, Vazquez-Ledo L, Novo-Veleiro I, González-Quintela A. Skin and soft-tissue infections: factors associated with mortality and re-admissions. Enferm Infecc Microbiol Clin. 2017;35(2):76–81.

    Article 
    PubMed 

    Google Scholar 

  • Talan D, Salhi B, Moran G, Mower W, Hsieh YH, Krishnadasan A, et al. Factors associated with the decision to hospitalize emergency department patients with a skin and soft tissue infection. West J Emerg Med. 2015;16(1):89–97.

    Article 
    PubMed 

    Google Scholar 

  • Cieri B, Conway EL, Sellick JA, Mergenhagen KA. Identification of risk factors for failure in patients with skin and soft tissue infections. Am J Emerg Med. 2019;37(1):48–52.

    Article 
    PubMed 

    Google Scholar 

  • Charlson ME, Pompei P, Ales KL, MacKenzie CR. A new method of classifying prognostic comorbidity in longitudinal studies: development and validation. J Chronic Dis. 1987;40(5):373–83.

    Article 
    PubMed 

    Google Scholar 

  • Jones AE, Trzeciak S, Kline JA. The sequential organ failure assessment score for predicting outcome in patients with severe sepsis and evidence of hypoperfusion at the time of emergency department presentation*. Crit Care Med. 2009;37(5):1649–54.

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Rhee JY, Kwon KT, Ki HK, Shin SY, Jung DS, Chung DR, et al. Scoring systems for prediction of mortality in patients with intensive care unit-acquired sepsis. Shock. 2009;31(2):146–50.

    Article 
    PubMed 

    Google Scholar 

  • Guidance for industry acute bacterial skin and skin structure infections: Developing drugs for treatment. 2013. Available from: https://www.fda.gov/drugs/guidance-compliance-regulatory-information/guidances-drugs. Cited 2 Aug 2024.

  • Parra Caballero P, Pérez Esteban S, Patiño Ruiz ME, Castañeda Sanz S, García Vadillo JA. Actualización en fascitis necrotizante. Seminarios de la Fundación Española de Reumatología. 2012;13(2):41–8.

    Article 

    Google Scholar 

  • Singer M, Deutschman CS, Seymour CW, Shankar-Hari M, Annane D, Bauer M, et al. The third international consensus definitions for sepsis and septic shock (Sepsis-3). JAMA. 2016;315(8):801.

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Barbier F, Timsit JF. Risk stratification for multidrug-resistant bacteria in patients with skin and soft tissue infection. Curr Opin Infect Dis. 2020;33(2):137–45.

    Article 
    PubMed 

    Google Scholar 

  • Keung EZ, Liu X, Nuzhad A, Adams C, Ashley SW, Askari R. Immunocompromised status in patients with necrotizing soft-tissue infection. JAMA Surg. 2013;148(5):419.

    Article 
    PubMed 

    Google Scholar 

  • The European Committee on Antimicrobial Susceptibility Testing. Breakpoint tables for interpretation of MICs and zone diameters. Version 13.0. 2023. http://www.eucast.org.

  • Stevens DL et al. Clin Infect Dis (2014; 59:147–59). Clinical Infectious Diseases. 2015;60(9):1448–1448.

  • Abetz JW, Adams NG, Mitra B. Skin and soft tissue infection management failure in the emergency department observation unit: a systematic review. Emerg Med J. 2018;35(1):56–61.

    Article 
    PubMed 

    Google Scholar 

  • Blanes Hernández R, Rodríguez Pérez M, Fernández Navarro J, Salavert Lletí M. Current approach to skin and soft tissue infections. Thinking about continuity of care. Rev Esp Quimioter. 2023;36(Suppl1):37–45.

    Article 
    PubMed 

    Google Scholar 

  • Sartelli M, Guirao X, Hardcastle TC, Kluger Y, Boermeester MA, Raşa K, et al. 2018 WSES/SIS-E consensus conference: recommendations for the management of skin and soft-tissue infections. World J Emerg Surg. 2018;13(1):58.

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Cyriac JM, James E. Switch over from intravenous to oral therapy: a concise overview. J Pharmacol Pharmacother. 2014;5(2):83–7.

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Bookstaver PB, Jenkins TC, Stenehjem E, Doron S, Brown J, Goldwater SH et al. Impact of outpatient vs inpatient ABSSSI treatment on outcomes: A retrospective observational analysis of medical charts across US emergency departments. Open Forum Infect Dis. 2018 May 15;5(7)ofy109.

  • Claeys KC, Zasowski EJ, Lagnf AM, Sabagha N, Levine DP, Davis SL, et al. Development of a risk-scoring tool to determine appropriate level of care in acute bacterial skin and skin structure infections in an acute healthcare setting. Infect Dis Ther. 2018;7(4):495–507.

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Gonçalves-Bradley DC, Lannin NA, Clemson L, Cameron ID, Shepperd S. Discharge planning from hospital. Cochrane Database Syst Reviews. 2022 Feb 24;2(2):CD000313.

  • Larney S, Peacock A, Mathers BM, Hickman M, Degenhardt L. A systematic review of injecting-related injury and disease among people who inject drugs. Drug Alcohol Depend. 2017;171:39–49.

    Article 
    PubMed 

    Google Scholar 

  • Biancarelli DL, Biello KB, Childs E, Drainoni M, Salhaney P, Edeza A, et al. Strategies used by people who inject drugs to avoid stigma in healthcare settings. Drug Alcohol Depend. 2019;198:80–6.

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Langham FJ, Curtis SJ, Tang MJ, Jomon B, Doyle JS, Vujovic O, et al. Acute injection-related infections requiring hospitalisation among people who inject drugs: clinical features, microbiology and management. Drug Alcohol Rev. 2022;41(7):1543–53.

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Ti L, Ti L. Leaving the hospital against medical advice among people who use illicit drugs: a systematic review. Am J Public Health. 2015;105(12):e53-9.

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Dunne MW, Talbot GH, Boucher HW, Wilcox M, Puttagunta S. Safety of dalbavancin in the treatment of skin and skin structure infections: a pooled analysis of randomized, comparative studies. Drug Saf. 2016;39(2):147–57.

    Article 
    PubMed 

    Google Scholar 

  • Vazquez Deida AA, Shihadeh KC, Preslaski CR, Young HL, Wyles DL, Jenkins TC. Use of a standardized Dalbavancin approach to facilitate earlier hospital discharge for vulnerable patients receiving prolonged inpatient antibiotic therapy. Open Forum Infect Dis. 2020 Jul 13;7(8):ofaa293.

  • Williams DN, Baker CA, Kind AC, Sannes MR. The history and evolution of outpatient parenteral antibiotic therapy (OPAT). Int J Antimicrob Agents. 2015;46(3):307–12.

    Article 
    PubMed 

    Google Scholar 

  • Mirón-Rubio M, Parra Jordán JJ, González Ramallo VJ, Mujal Martínez A, Fernández Muixí J, Iglesias Gallego M, et al. Economic burden of skin and skin structure infections due to gram-positive bacteria in patients on hospital at home-based outpatient parenteral antibiotic therapy (OPAT). Rev Esp Quimioter. 2023;36(3):291–301.

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Chan M, Ooi CK, Wong J, Zhong L, Lye D. Role of outpatient parenteral antibiotic therapy in the treatment of community acquired skin and soft tissue infections in Singapore. BMC Infect Dis. 2017;17(1):474.

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Seaton RA, Sharp E, Bezlyak V, Weir CJ. Factors associated with outcome and duration of therapy in outpatient parenteral antibiotic therapy (OPAT) patients with skin and soft-tissue infections. Int J Antimicrob Agents. 2011;38(3):243–8.

    Article 
    PubMed 

    Google Scholar 

  • Ong BS, Ngian VJJ, Yeong C, Keighley C.

    Out Of Hospital And In Hospital Management Of Cellulitis Requiring Intravenous Therapy

    . Int J Gen Med. 2019;12:447–53. 

  • Huang V, Ruhe JJ, Lerner P, Fedorenko M. Risk factors for readmission in patients discharged with outpatient parenteral antimicrobial therapy: a retrospective cohort study. BMC Pharmacol Toxicol. 2018;19(1):50.

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Suzuki J, Johnson J, Montgomery M, Hayden M, Price C. Outpatient parenteral antimicrobial therapy among people who inject drugs: a review of the literature. Open Forum Infect Dis. 2018 Aug 7;5(9):ofy194.

  • Buehrle DJ, Shields RK, Shah N, Shoff C, Sheridan K. Risk factors associated with outpatient parenteral antibiotic therapy program failure among intravenous drug users. Open Forum Infect Dis. 2017 May 23;4(3)ofx102.

  • Moet GJ, Jones RN, Biedenbach DJ, Stilwell MG, Fritsche TR. Contemporary causes of skin and soft tissue infections in North America, Latin America, and europe: report from the SENTRY antimicrobial surveillance program (1998–2004). Diagn Microbiol Infect Dis. 2007;57(1):7–13.

    Article 
    PubMed 

    Google Scholar 

  • Ray GT, Suaya JA, Baxter R. Incidence, microbiology, and patient characteristics of skin and soft-tissue infections in a U.S. population: a retrospective population-based study. BMC Infect Dis. 2013;13(1):252.

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Vella V, Galgani I, Polito L, Arora AK, Creech CB, David MZ, et al. Staphylococcus aureus skin and soft tissue infection recurrence rates in outpatients: A retrospective database study at 3 US medical centers. Clin Infect Dis. 2021;73(5):e1045–53.

    Article 
    PubMed 

    Google Scholar 

  • Collazos J, de la Fuente B, García A, Gómez H, Menéndez C, Enríquez H, et al. Cellulitis in adult patients: a large, multicenter, observational, prospective study of 606 episodes and analysis of the factors related to the response to treatment. PLoS One. 2018;13(9):e0204036.

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Leme RCP, Bispo PJM, Salles MJ. Community-genotype methicillin-resistant Staphylococcus aureus skin and soft tissue infections in Latin America: a systematic review. Braz J Infect Dis. 2021;25(1):101539.

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Cucinotta D, Vanelli M WHO declares COVID-19 a pandemic.Acta Biomed. 2020; 91((1):):157–60.

    PubMed 
    PubMed Central 

    Google Scholar 

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  • CIArb Guidelines on AI: Key Soft Law Step in Arbitration – Natalia Chumak – Signature Litigation

    1. CIArb Guidelines on AI: Key Soft Law Step in Arbitration – Natalia Chumak  Signature Litigation
    2. AI Arbitrators Will Destroy the Legal Profession (And That’s a Good Thing)  JD Supra
    3. Stop worrying and learn to love AI  Wisconsin Law Journal
    4. Commentary: AI and the Legal Profession at a Crossroads  Caixin Global
    5. When AI’s the Arbiter, What Role Do Humans Play?  Commercial Search

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  • Innovative CRISPR strategy resensitizes lung cancer to treatment

    Innovative CRISPR strategy resensitizes lung cancer to treatment

    In a major step forward for cancer care, researchers at ChristianaCare’s Gene Editing Institute have shown that disabling the NRF2 gene with CRISPR technology can reverse chemotherapy resistance in lung cancer. The approach restores drug sensitivity and slows tumor growth. The findings appear today in the journal Molecular Therapy Oncology.

    This breakthrough stems from more than a decade of research by the Gene Editing Institute into the NRF2 gene, a known driver of treatment resistance. The results were consistent across multiple in vitro studies using human lung cancer cell lines and in vivo animal models.

    We’ve seen compelling evidence at every stage of research. It’s a strong foundation for taking the next step toward clinical trials.”


    Kelly Banas, Ph.D., lead author of the study and associate director of research, Gene Editing Institute

    Potential beyond lung cancer

    The study focused on lung squamous cell carcinoma, an aggressive and common form of non-small cell lung cancer (NSCLC) that accounts for 20% to 30% of all lung cancer cases, according to the American Cancer Society. It’s estimated that over 190,000 people in the U.S. will be diagnosed in 2025.

    While the research centered on this cancer type, the implications are broader. Overactive NRF2 contributes to chemotherapy resistance in several solid tumors, including liver, esophageal and head and neck cancers. The results suggest a CRISPR-based strategy targeting NRF2 could help resensitize a wide range of treatment-resistant tumors to standard chemotherapy.

    “This is a significant step toward overcoming one of the biggest challenges in cancer therapy – drug resistance,” Banas said. “By targeting a key transcription factor that drives resistance, we’ve shown that gene editing can re-sensitize tumors to standard treatment. We’re hopeful that in clinical trials and beyond, this is what will allow chemotherapy to improve outcomes for patients and could enable them to remain healthier during the entirety of their treatment regimen.”

    Targeting a master switch for resistance

    The research zeroed in on a tumor-specific mutation, R34G, in the NRF2 gene, which acts as a master regulator of cellular stress responses. When overactive, NRF2 helps cancer cells withstand chemotherapy.

    Using CRISPR/Cas9, the team engineered lung cancer cells with the R34G mutation and successfully knocked out NRF2. This restored sensitivity to chemotherapy drugs such as carboplatin and paclitaxel. In animal models, tumors directly treated with CRISPR to knockout NRF2 grew more slowly and responded better to treatment.

    “This work brings transformational change to how we think about treating resistant cancers,” said Eric Kmiec, Ph.D., senior author of the study and executive director of the Gene Editing Institute. “Instead of developing entirely new drugs, we are using gene editing to make existing ones effective again.”

    Editing reaches threshold levels

    One of the most promising discoveries was that disrupting NRF2 in just 20% to 40% of tumor cells, was enough to improve the response to chemotherapy and shrink tumors. This insight is particularly relevant for clinical use, where editing every cancer cell may not be feasible.

    To test therapy in mice, the researchers used lipid nanoparticles (LNPs), a non-viral method with high efficiency and low risk of unintended, off-target effects. Sequencing confirmed that the edits were highly specific to the mutated NRF2 gene, with minimal unintended changes elsewhere in the genome.

    “The power of this CRISPR therapy lies in its precision. It’s like an arrow that hits only the bullseye,” said Banas. “This level of specificity with minimal unanticipated genomic side effects offers real hope for the cancer patients who could one day receive this treatment.”

    Source:

    ChristianaCare Gene Editing Institute

    Journal reference:

    Banas, K. H., et al. (2025). Functional Characterization of Tumor-Specific CRISPR-Directed Gene Editing as a Combinatorial Therapy for the Treatment of Solid Tumors. Molecular Therapy Oncology. doi:10.1016/j.omton.2025.201079. https://www.sciencedirect.com/science/article/pii/S2950329925001481?via%3Dihub

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  • Capturing the spatial structure of the benthic microbiome under an intensive aquaculture scenario in Chilean Patagonia | BMC Microbiology

    Capturing the spatial structure of the benthic microbiome under an intensive aquaculture scenario in Chilean Patagonia | BMC Microbiology

  • Verhoeven JTP, Salvo F, Knight R, Hamoutene D, Dufour SC. Temporal bacterial surveillance of salmon aquaculture sites indicates a long lasting benthic impact with minimal recovery. Front Microbiol. 2018;9:1–13.

    Google Scholar 

  • Miranda RM, Aguila-Torres P, Aranda CP, Maldonado J, Casado A. Taxonomy and diversity of bacterial communities associated with marine sediments from Chilean salmonid farms. Aquac Res. 2021;52:1605–20.

    Google Scholar 

  • Lindström ES, Langenheder S. Local and regional factors influencing bacterial community assembly. Environ Microbiol Rep. 2012;4:1–9.

    PubMed 

    Google Scholar 

  • Logares R, Lindström ES, Langenheder S, Logue JB, Paterson H, Laybourn-Parry J, et al. Biogeography of bacterial communities exposed to progressive long-term environmental change. ISME J. 2013;7:937–48.

    PubMed 

    Google Scholar 

  • Clay CG, Dunhill AM, Reimer JD, Beger M. Trait networks: assessing marine community resilience and extinction recovery. iScience. 2024;27:1–10.

    Google Scholar 

  • Keyes AA, McLaughlin JP, Barner AK, Dee LE. An ecological network approach to predict ecosystem service vulnerability to species losses. Nat Commun. 2021;12:1–11.

    Google Scholar 

  • Fuhrman JA, Cram JA, Needham DM. Marine microbial community dynamics and their ecological interpretation. Nat Rev Microbiol. 2015;14. https://doi.org/10.1038/nrmicro3417.

  • Rodríguez J, Gallampois CMJ, Haglund P, Timonen S, Rowe O. Bacterial communities as indicators of environmental pollution by POPs in marine sediments. Environ Pollut. 2021;268:1–11.

    Google Scholar 

  • Ko KKK, Chng KR, Nagarajan N. Metagenomics-enabled microbial surveillance. Nat Microbiol. 2022;7:486–96.

    PubMed 

    Google Scholar 

  • Urbina MA. Temporal variation on environmental variables and pollution indicators in marine sediments under sea salmon farming cages in protected and exposed zones in the Chilean inland Southern sea. Sci Total Environ. 2016;573:841–53.

    PubMed 

    Google Scholar 

  • Tomova A, Ivanova L, Buschmann AH, Rioseco ML, Kalsi RK, Godfrey HP, et al. Antimicrobial resistance genes in marine bacteria and human uropathogenic Escherichia coli from a region of intensive aquaculture. Environ Microbiol Rep. 2015;7:803–9.

    PubMed 

    Google Scholar 

  • Tucca F, Moya H, Pozo K, Borghini F, Focardi S, Barra R. Occurrence of antiparasitic pesticides in sediments near salmon farms in the Northern Chilean Patagonia. Mar Pollut Bull. 2017;115:465–8.

    PubMed 

    Google Scholar 

  • Billi M, Mascareño A, Henríquez PA, Rodríguez I, Padilla F, Ruz GA. Learning from crises? The long and winding road of the salmon industry in Chiloé Island, Chile. Mar Policy. 2022;140:1–16.

    Google Scholar 

  • Soto D, León-Muñoz J, Dresdner J, Luengo C, Tapia FJ, Garreaud R. Salmon farming vulnerability to climate change in Southern Chile: understanding the biophysical, socioeconomic and governance links. Rev Aquacult. 2019;11:354–74.

    Google Scholar 

  • Linford P, Pérez-Santos I, Montero P, Díaz PA, Aracena C, Pinilla E, et al. Oceanographic processes driving low-oxygen conditions inside Patagonian fjords. Biogeosciences. 2024;21:1433–59.

    Google Scholar 

  • Cabello FC, Godfrey HP, Buschmann AH, Dölz HJ. Aquaculture as yet another environmental gateway to the development and globalisation of antimicrobial resistance. Lancet Infect Dis. 2016;16:e127-33.

    PubMed 

    Google Scholar 

  • Skliros D, Kostakou M, Kokkari C, Tsertou MI, Pavloudi C, Zafeiropoulos H, et al. Unveiling emerging opportunistic fish pathogens in aquaculture: A comprehensive seasonal study of microbial composition in mediterranean fish hatcheries. Microorganisms. 2024;12:1–22.

    Google Scholar 

  • Liu J, Meng Z, Liu X, Zhang XH. Microbial assembly, interaction, functioning, activity and diversification: a review derived from community compositional data. Mar Life Sci Technol. 2019;1(1):112–28.

    Google Scholar 

  • Ortiz P, Quiroga E, Montero P, Hamame M, Betti F. Trophic structure of benthic communities in a Chilean fjord (45°S) influenced by salmon aquaculture: insights from stable isotopic signatures. Mar Pollut Bull. 2021;173:1–9.

    Google Scholar 

  • Soto D, León-Muñoz J, Garreaud R, Quiñones RA, Morey F. Scientific warnings could help to reduce farmed salmon mortality due to harmful algal blooms. Mar Policy. 2021;132:1–5.

    Google Scholar 

  • Cabello FC, Godfrey HP, Tomova A, Ivanova L, Dölz H, Millanao A, et al. Antimicrobial use in aquaculture re-examined: its relevance to antimicrobial resistance and to animal and human health. Environ Microbiol. 2013;15:1917–42.

    PubMed 

    Google Scholar 

  • Kingsbury MV, Hamoutene D, Kraska P, Lacoursière-Roussel A, Page F, Coyle T, et al. Relationship between in feed drugs, antibiotics and organic enrichment in marine sediments at Canadian Atlantic salmon aquaculture sites. Mar Pollut Bull. 2023;188:1–17.

    Google Scholar 

  • Inostroza PA, Jessen GL, Li F, Zhang X, Brack W, Backhaus T. Multi-compartment impact of micropollutants and particularly antibiotics on bacterial communities using environmental DNA at river basin-level. Environ Pollut. 2025;366:1–10.

    Google Scholar 

  • Aminot Y, Sayfritz SJ, Thomas KV, Godinho L, Botteon E, Ferrari F, et al. Environmental risks associated with contaminants of legacy and emerging concern at European aquaculture areas. Environ Pollut. 2019;252:1301–10.

    PubMed 

    Google Scholar 

  • Veloso S, Amouroux D, Lanceleur L, Cagnon C, Monperrus M, Deborde J, et al. Keystone microbial taxa organize micropollutant-related modules shaping the microbial community structure in estuarine sediments. J Hazard Mater. 2023;448:1–13.

    Google Scholar 

  • Previšić A, Rožman M, Mor JR, Acuña V, Serra-Compte A, Petrović M, et al. Aquatic macroinvertebrates under stress: bioaccumulation of emerging contaminants and metabolomics implications. Sci Total Environ. 2020;704:1–12.

    Google Scholar 

  • Najafpour B, Pinto PIS, Sanz EC, Martinez-Blanch JF, Canario AVM, et al. Core Microbiome profiles and their modification by environmental, biological, and rearing factors in aquaculture hatcheries. Mar Pollut Bull. 2023;193:1–16.

    Google Scholar 

  • Klase G, Lee S, Liang S, Kim J, Zo YG, Lee J. The microbiome and antibiotic resistance in integrated fishfarm water: implications of environmental public health. Sci Total Environ. 2019;649:1491–501.

    PubMed 

    Google Scholar 

  • Feng Y, Lu Y, Chen Y, Xu J, Jiang J. Microbial community structure and antibiotic resistance profiles in sediments with long-term aquaculture history. J Environ Manage. 2023;341:1–12.

    Google Scholar 

  • Frühe L, Dully V, Forster D, Keeley NB, Laroche O, Pochon X, et al. Global trends of benthic bacterial diversity and community composition along organic enrichment gradients of salmon farms. Front Microbiol. 2021;12:1–17.

    Google Scholar 

  • Almeida DB, Semedo M, Magalhães C, Blanquet I, Mucha AP. The network of nitrifying and pathogenic prokaryotic interactions in a recirculating aquaculture system of a sole (Solea senegalensis) hatchery. Front Mar Sci. 2022;9:1–11.

  • Ortiz-Severín J, Hodar C, Stuardo C, Aguado-Norese C, Maza F, González M, et al. Impact of salmon farming in the antibiotic resistance and structure of marine bacterial communities from surface seawater of a Northern Patagonian area of Chile. Biol Res. 2024;57:1–19.

    Google Scholar 

  • Tamayo-Leiva J, Cifuentes-Anticevic J, Aparicio-Rizzo P, Arroyo JI, et al. Influence of estuarine water on the microbial community structure of Patagonian Fjords. Front Mar Sci. 2021;8:1–16.

    Google Scholar 

  • Hornick KM, Buschmann AH. Insights into the diversity and metabolic function of bacterial communities in sediments from Chilean salmon aquaculture sites. Ann Microbiol. 2018;68:63–77.

    Google Scholar 

  • Pawlowski J, Bruce K, Aguirre KP. Environmental DNA metabarcoding for benthic monitoring: A review of sediment sampling and DNA extraction methods. Sci Total Environ J. 2022;818:1–17.

    Google Scholar 

  • Inostroza PA, Soriano Y, Carmona E, Krauss M, et al. Preliminary dataset of emerging contaminants in surface water, bottom water, porewater, and sediment: urban and aquaculture impacts in Coliumo Bay and Caucahue channel in the central and Southern Coast of Chile. Data Br. 2024;55:1–10.

    Google Scholar 

  • Buschmann AH, Hernández-González MC, Aranda C, Chopin T, Neori A, Halling C, et al. In: Fath BD, editor. Mariculture waste Management. SE Jørgensen. Oxford: Encycl Ecol Elsevier; 2008. pp. 2211–7.

    Google Scholar 

  • Nilsen T, Pettersen R, Keeley NB, Ray JL, Majaneva S, et al. Association of microbial networks with the coastal seafloor macrofauna ecological state. Environ Sci Technol. 2025;59:7517–29.

    PubMed 
    PubMed Central 

    Google Scholar 

  • Iriarte JL, Gonzlez HE, Nahuelhual L. Patagonian Fjord ecosystems in Southern Chile as a highly vulnerable region: problems and needs. Ambio. 2010;39:463–6.

    PubMed 
    PubMed Central 

    Google Scholar 

  • Pérez-Santos I, Díaz PA, Silva N, Garreaud R, Montero P, Henríquez-Castillo C, et al. Oceanography time series reveals annual asynchrony input between oceanic and estuarine waters in Patagonian Fjords. Sci Total Environ. 2021;798:1–18.

    Google Scholar 

  • Quiñones RA, Fuentes M, Montes RM, Soto D, León-Muñoz J. Environmental issues in Chilean salmon farming: a review. Rev Aquacult. 2019;11:375–402.

    Google Scholar 

  • Buschmann AH, Niklitschek EJ, Pereda SV. Aquaculture and its impacts on the conservation of Chilean patagonia. In: In Conservation in Chilean patagonia: assessing the state of Knowledge, Opportunities, and challenges. Cham: Springer International Publishing; 2023. p. 303–20.

    Google Scholar 

  • de Castro-Català N, Kuzmanovic M, Roig N, Sierra J, Ginebreda A, Barceló D, et al. Ecotoxicity of sediments in rivers: invertebrate community, toxicity bioassays and the toxic unit approach as complementary assessment tools. Sci Total Environ. 2016;540:297–306.

    PubMed 

    Google Scholar 

  • Lagier JC, Dubourg G, Million M, Cadoret F, Bilen M, Fenollar F, et al. Culturing the human microbiota and culturomics. Nat Rev Microbiol. 2018;16:540–50.

    PubMed 

    Google Scholar 

  • Muyzer G, Waal ECDE, Uitierlinden AG. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain Reaction-Amplified genes coding for 16S rRNA. Appl Environ Microbiol. 1993;59:695–700.

  • Caporaso JG, Lauber CL, Walters WA, Berg-lyons D, Lozupone CA, Turnbaugh PJ, et al. Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample. Proc Natl Acad Sci U S A. 2011;108:4516–22.

    PubMed 

    Google Scholar 

  • Straub D, Blackwell N, Langarica-Fuentes A, Peltzer A, Nahnsen S, Kleindienst S. Interpretations of environmental microbial community studies are biased by the selected 16S rRNA (gene) amplicon sequencing pipeline. Front Microbiol. 2020;11:1–18.

    Google Scholar 

  • Ewels PA, Peltzer A, Fillinger S, Patel H, Johannes A, Andreas W, et al. The nf-core framework for community-curated bioinformatics pipelines. Nat Biotechnol. 2020;38:271–8.

    Google Scholar 

  • Grüning B, Dale R, Sjödin A, Chapman BA, Rowe J, Tomkins-Tinch CH, et al. Bioconda: sustainable and comprehensive software distribution for the life sciences. Nat Methods. 2018;15:475–6.

    PubMed 
    PubMed Central 

    Google Scholar 

  • da Veiga Leprevost F, Grüning BA, Alves Aflitos S, Röst HL, et al. BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics. 2017;33:2580–2.

    PubMed 
    PubMed Central 

    Google Scholar 

  • Quast C, Pruesse E, Yilmaz P, Gerken J, Schweer T, Yarza P, et al. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res. 2013;41:590–6.

    Google Scholar 

  • Lundin D, Andersson A. SBDI Sativa curated 16S GTDB database. SciLifeLab Data Repos. 2021. https://figshare.scilifelab.se/articles/dataset/SBDI_Sativa_curated_16S_GTDB_database/14869077

  • Bolyen E, Rideout JR, Dillon MR, Bokulich NA, Abnet CC, Al-Ghalith GA, et al. Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2. Nat Biotechnol. 2019;37:852–7.

    PubMed 
    PubMed Central 

    Google Scholar 

  • Wickham H. Elegant graphics for data analysis (2nd ed). 2017;77:3–5.

    Google Scholar 

  • McMurdie PJ, Holmes S. Phyloseq: an R package for reproducible interactive analysis and graphics of microbiome census data. PLoS ONE. 2013;8:e61217.

    PubMed 
    PubMed Central 

    Google Scholar 

  • Stanimirova I, Walczak B, Massart DL, Simeonov V. A comparison between two robust PCA algorithms. Chemom Intell Lab Syst. 2004;71:83–95.

    Google Scholar 

  • Rohart F, Gautier B, Singh A, Lê Cao K-A, mixOmics. An R package for ‘omics feature selection and multiple data integration. PLoS Comput Biol. 2017;13:1–14.

    Google Scholar 

  • Dhariwal A, Chong J, Habib S, King IL, Agellon LB, Xia J, et al. A web-based tool for comprehensive statistical, visual and meta-analysis of microbiome data. Nucleic Acids Res. 2017;45:W180-8.

    PubMed 
    PubMed Central 

    Google Scholar 

  • Segata N, Izard J, Waldron L, Gevers D, Miropolsky L, Garrett WS, et al. Metagenomic biomarker discovery and explanation. Genome Biol. 2011;12:1–18.

    Google Scholar 

  • Berry D, Widder S. Deciphering microbial interactions and detecting keystone species with co-occurrence networks. Front Microbiol. 2014;5:1–14.

    Google Scholar 

  • Röttjers L, Faust K. From hairballs to hypotheses–biological insights from microbial networks. FEMS Microbiol Rev. 2018;42:761–80.

    PubMed 
    PubMed Central 

    Google Scholar 

  • Bastian M, Heymann S. Gephi: an open source software for exploring and manipulating networks. ICWSM. 2009;8:361–2.

    Google Scholar 

  • Gutiérrez MH, Galand PE, Moffat C. Melting glacier impacts community structure of bacteria, archaea and fungi in a Chilean Patagonia fjord. Environ Microbiol. 2015;17:3882–97.

    PubMed 

    Google Scholar 

  • Schneider W, Pérez-Santos I, Ross L, Bravo L, Seguel R, Hernández F. On the hydrography of Puyuhuapi Channel, Chilean Patagonia. Prog Oceanogr. 2014;129:8–18.

    Google Scholar 

  • Montero P, Gutiérrez MH, Daneri G, Jacob B. The effect of salmon Food-Derived DOM and glacial melting on activity and diversity of free-living bacterioplankton in Chilean Patagonian Fjords. Front Microbiol. 2022;12:1–21.

    Google Scholar 

  • Liu JJ, Diao ZH, Xu XR, Xie Q. Effects of dissolved oxygen, salinity, nitrogen and phosphorus on the release of heavy metals from coastal sediments. Sci Total Environ. 2019;666:894–901.

    PubMed 

    Google Scholar 

  • Stojan I, Šantić D, Villena-Alemany C, Trumbić Ž, Matić F, Vrdoljak Tomaš A, et al. Ecology of aerobic anoxygenic phototrophs on a fine-scale taxonomic resolution in Adriatic sea unravelled by unsupervised neural network. Environ Microbiome. 2024;19:1–20.

    Google Scholar 

  • Sipler RE, Kellogg CTE, Connelly TL, Roberts QN, et al. Microbial community response to terrestrially derived dissolved organic matter in the coastal Arctic. Front Microbiol. 2017;8:1–19.

    Google Scholar 

  • Gutiérrez MH, Narváez D, Daneri G, Montero P, Pérez-Santos I, Pantoja S. Linking seasonal reduction of microbial diversity to increase in winter temperature of waters of a Chilean patagonia fjord. Front Mar Sci. 2018;5:1–20.

    Google Scholar 

  • León-Muñoz J, Aguayo R, Corredor-Acosta A, Tapia FJ, Iriarte JL, Reid B, et al. Hydrographic shifts in coastal waters reflect climate-driven changes in hydrological regimes across Northwestern patagonia. Sci Rep. 2024;14:1–16.

    Google Scholar 

  • Tamme R, Hiiesalu I, Laanisto L, Szava-Kovats R, Pärtel M. Environmental heterogeneity, species diversity and co-existence at different spatial scales. J Veg Sci. 2010;21:796–801.

    Google Scholar 

  • Maturana-Martínez C, Fernández C, González HE, Galand PE. Different active microbial communities in two contrasted subantarctic fjords. Front Microbiol. 2021;12:1–14.

    Google Scholar 

  • Sun J, Zhou H, Cheng H, Chen Z, Wang Y. Bacterial abundant taxa exhibit stronger environmental adaption than rare taxa in the Arctic Ocean sediments. Mar Environ Res. 2024;199:1–10.

    Google Scholar 

  • Choi A, Lee TK, Cho H, Lee WC, Hyun JH. Shifts in benthic bacterial communities associated with farming stages and a microbiological proxy for assessing sulfidic sediment conditions at fish farms. Mar Pollut Bull. 2022;178:1–13.

    Google Scholar 

  • Borer B, Ciccarese D, Johnson D, Or D. Spatial organization in microbial range expansion emerges from trophic dependencies and successful lineages. Commun Biol. 2020;3:1–10.

    Google Scholar 

  • Cremin K, Duxbury SJN, Rosko J, Soyer OS. Formation and emergent dynamics of spatially organized microbial systems. Interface Focus. 2023;13:1–11.

    Google Scholar 

  • Iriarte JL. Natural and human influences on marine processes in Patagonian subantarctic coastal waters. Front Mar Sci. 2018;5:1–7.

    Google Scholar 

  • Neu AT, Allen EE, Roy K. Defining and quantifying the core microbiome: challenges and prospects. Proc Natl Acad Sci U S A. 2021;118:1–10.

    Google Scholar 

  • Sun F, Wu M, Wang Y, Sun C, Xu Z. Diversity and potential function of bacterial communities in different upwelling systems. Estuar Coast Shelf Sci. 2020;237:106698.

    Google Scholar 

  • Ramos-Mendoza IS, Embarcadero-Jiménez S, Barrios-Navarro AF, et al. Prokaryotic community structure and predicted metabolism associated with hydrocarbon degradation in marine sediments from the Northwest Coast of Baja California, Mexico. J Soils Sediments. 2024;24:3148–66.

    Google Scholar 

  • Choi A, Cho H, Kim B, Kim HC, Jung RH, Lee WC, et al. Effects of finfish aquaculture on biogeochemistry and bacterial communities associated with sulfur cycles in highly sulfidic sediments. Aquacult Environ Interact. 2018;10:413–27.

    Google Scholar 

  • Hoffmann K, Bienhold C, Buttigieg PL, Knittel K, Laso-Pérez R, Rapp JZ, et al. Diversity and metabolism of woeseiales bacteria, global members of marine sediment communities. ISME J. 2020;14:1042–56.

    PubMed 
    PubMed Central 

    Google Scholar 

  • Broman E, Sjöstedt J, Pinhassi J, Dopson M. Shifts in coastal sediment oxygenation cause pronounced changes in microbial community composition and associated metabolism. Microbiome. 2017;5:96.

    PubMed 
    PubMed Central 

    Google Scholar 

  • Davenport J. The marine ecology of the Laguna San Rafael (Southern Chile): ice scour and opportunism. Estuar Coast Shelf Sci. 1995;41:21–37.

    Google Scholar 

  • Martinez-Varela A, Casas G, Berrojalbiz N, Lundin D, Piña B, Dachs J, et al. Metatranscriptomic responses and microbial degradation of background polycyclic aromatic hydrocarbons in the coastal mediterranean and Antarctica. Environ Sci Pollut Res Int. 2023;30:119988–99.

    PubMed 
    PubMed Central 

    Google Scholar 

  • Vila-Costa M, Cerro-Gálvez E, Martínez-Varela A, Casas G, Dachs J. Anthropogenic dissolved organic carbon and marine microbiomes. ISME J. 2020;14:2646–8.

    PubMed 
    PubMed Central 

    Google Scholar 

  • Jessen GL, Lichtschlag A, Ramette A, Pantoja S, Rossel PE, Schubert CJ, et al. Hypoxia causes preservation of labile organic matter and changes seafloor microbial community composition (Black Sea). Sci Adv. 2017;3:1–14.

    Google Scholar 

  • Molina V, Cornejo-D’Ottone M, Soto EH, Quiroga E, Alarcón G, Silva D, et al. Biogeochemical responses and seasonal dynamics of the benthic boundary layer microbial communities during the El Niño 2015 in an Eastern boundary upwelling system. Water. 2021;13:1–18.

    Google Scholar 

  • Zárate A, Dorador C, Valdés J, Molina V, Icaza G, Pacheco AS, et al. Benthic microbial diversity trends in response to heavy metals in an oxygen-deficient eutrophic Bay of the Humboldt current system offshore the Atacama desert. Environ Pollut. 2021;286:1–12.

    Google Scholar 

  • Feng Y, Hu J, Chen Y, Xu J, Yang B, Jiang J. Ecological effects of antibiotics on aquaculture ecosystems based on microbial community in sediments. Ocean Coast Manag. 2022;224:1–12.

    Google Scholar 

  • Aldeguer-Riquelme B, Rubio-Portillo E, Álvarez-Rogel J, Giménez-Casalduero F, Otero XL, Belando MD, et al. Factors structuring microbial communities in highly impacted coastal marine sediments (Mar Menor lagoon, SE Spain). Front Microbiol. 2022;13:1–18.

    Google Scholar 

  • Mitrović M, Kostešić E, Marković T, Selak L, Hausmann B, Pjevac P, et al. Microbial community composition and hydrochemistry of underexplored geothermal waters in Croatia. Syst Appl Microbiol. 2022. https://doi.org/10.1016/j.syapm.2022.126359.

    Article 
    PubMed 

    Google Scholar 

  • Bahram M, Anslan S, Hildebrand F, Bork P, Tedersoo L. Newly designed 16S rRNA metabarcoding primers amplify diverse and novel archaeal taxa from the environment. Environ Microbiol Rep. 2019;11:487–94.

    PubMed 

    Google Scholar 

  • Biddle JF, Lipp JS, Lever MA, Lloyd KG, Sørensen KB, Anderson R, et al. Heterotrophic archaea dominate sedimentary subsurface ecosystems off Peru. Proc Natl Acad Sci U S A. 2006;103:3846–51.

    PubMed 
    PubMed Central 

    Google Scholar 

  • Dong X, Peng Y, Wang M, Woods L, Wu W, Wang Y, et al. Evolutionary ecology of microbial populations inhabiting deep sea sediments associated with cold seeps. Nat Commun. 2023;14:1–13.

    Google Scholar 

  • Newton RJ, Bootsma MJ, Morrison HG, Sogin ML, McLellan SL. A microbial signature approach to identify fecal pollution in the waters off an urbanized coast of Lake Michigan. Microb Ecol. 2013;65:1011–23.

    PubMed 
    PubMed Central 

    Google Scholar 

  • Fogarty C, Burgess CM, Cotter PD, Cabrera-Rubio R, Whyte P, Smyth C, et al. Diversity and composition of the gut microbiota of Atlantic salmon (Salmo salar) farmed in Irish waters. J Appl Microbiol. 2019;127:648–57.

    PubMed 

    Google Scholar 

  • Jurelevicius D, Cotta SR, Montezzi LF, Dias ACF, Mason OU, Picão RC, et al. Enrichment of potential pathogens in marine microbiomes with different degrees of anthropogenic activity. Environ Pollut. 2021;268:1–12.

    Google Scholar 

  • Foysal MJ, Kawser AQMR, Paul SI, Chaklader MR, Gupta SK, Tay A, et al. Prevalence of opportunistic pathogens and anti-microbial resistance in urban aquaculture ponds. J Hazard Mater. 2024;474:1–12.

    Google Scholar 

  • Li B, Yang Y, Ma L, Ju F, Guo F, Tiedje JM, et al. Metagenomic and network analysis reveal wide distribution and co-occurrence of environmental antibiotic resistance genes. ISME J. 2015;9:2490–502.

    PubMed 
    PubMed Central 

    Google Scholar 

  • Zhang C, Liu Q, Li X, Wang M, Liu X, Yang J, et al. Spatial patterns and co-occurrence networks of microbial communities related to environmental heterogeneity in deep-sea surface sediments around Yap Trench, Western Pacific ocean. Sci Total Environ. 2021;759:1–14.

    Google Scholar 

  • von Hoyningen-Huene AJE, Schneider D, Fussmann D, Reimer A, Arp G, Daniel R. DNA- and RNA-based bacterial communities and geochemical zonation under changing sediment Porewater dynamics on the Aldabra Atoll. Sci Rep. 2022;12:1–16.

    Google Scholar 

  • Petro C, Starnawski P, Schramm A, Kjeldsen KU. Microbial community assembly in marine sediments. Aquat Microb Ecol. 2017;79:177–95.

    Google Scholar 

  • Laroche O, Pochon X, Tremblay LA, Ellis JI, Lear G, Wood SA. Incorporating molecular-based functional and co-occurrence network properties into benthic marine impact assessments. FEMS Microbiol Ecol. 2018;94:1–12.

    Google Scholar 

  • Gomathinayagam S, Kanagalingam S, Chandrasekaran S, Krishnan T, Muthukaliannan GK. Millennial-scale microbiome analysis reveals ancient antimicrobial resistance conserved despite modern selection pressures. Environ Microbiome. 2024;19:1–12.

    Google Scholar 

  • Buschmann AH, Tomova A, López A, Maldonado MA, Henríquez LA, Ivanova L, et al. Salmon aquaculture and antimicrobial resistance in the marine environment. PLoS ONE. 2012;7:26–8.

    Google Scholar 

  • Meyer JM, Leempoel K, Losapio G, Hadly EA. Molecular ecological network analyses: an effective conservation tool for the assessment of biodiversity, trophic interactions, and community structure. Front Ecol Evol. 2020;8:1–19.

    Google Scholar 

  • Dully V, Balliet H, Frühe L, Däumer M, Thielen A, Gallie S, et al. Robustness, sensitivity and reproducibility of eDNA metabarcoding as an environmental biomonitoring tool in coastal salmon aquaculture – An inter-laboratory study. Ecol Indic. 2021;121:1–15.

    Google Scholar 

  • Lozano-Muñoz I, Wacyk J, Kretschmer C, Vásquez-Martínez Y, Martin MCS. Antimicrobial resistance in Chilean marine-farmed salmon: improving food safety through one health. One Health. 2021. https://doi.org/10.1016/j.onehlt.2021.100219.

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Rodríguez-Luna D, Vela N, Alcalá FJ, Encina-Montoya F. The environmental impact assessment in aquaculture projects in Chile: a retrospective and prospective review considering cultural aspects. Sustain. 2021;13:1–19.

    Google Scholar 

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  • EU awards over €600 million to alternative fuel projects to boost zero-emission mobility

    EU awards over €600 million to alternative fuel projects to boost zero-emission mobility

    70 projects are receiving over €600 million in EU grants to electrify and decarbonise road, maritime, inland waterway and air transport along the trans-European transport network (TEN-T). These projects will deploy alternative fuels supply infrastructure such as electric recharging stations, hydrogen refuelling stations, electricity supply and ammonia and methanol bunkering facilities across 24 EU countries.

    Europe’s transport network will be electrified through the installation of more than 1 000 electric recharging points for light-duty vehicles with a capacity of 150 kW. 2 000 new recharging points for heavy-duty vehicles will deliver a capacity of 350kW and 586 recharging points with a 1 MW power output. Additionally, 16 European airports will electrify their ground handling services, making a key contribution towards reducing aviation emissions.

    The hydrogen economy will also be boosted through the installation of 38 hydrogen refuelling stations for cars, trucks and buses. Finally, 24 maritime ports will benefit from the integration of greener technologies, including Onshore Power Supply (OPS), electrification of port services and ammonia bunkering facilities to fuel maritime vessels. 

    Commissioner for Sustainable Transport and Tourism Apostolos Tzitzikostas said:  

    We are currently supporting 70 projects with €600 million in EU funding to accelerate the deployment of alternative fuels infrastructure across Europe. These investments will strengthen our competitiveness and help make the transition to zero-emission mobility easier and more accessible for all citizens.

    Paloma Aba Garrote, Director of the European Climate, Infrastructure and Environment Executive Agency added: 

    This significant EU support for public and private organisations will accelerate the transport sector’s transition toward a sustainable future. With these new projects, more than €2.5 billion in EU grants has been allocated to alternative fuels projects through AFIF since 2021. This demonstrates EU’s ambition to make zero-emission mobility an everyday reality.

    Next steps

    Following the approval of the 70 selected projects by the EU Member States on 13 November 2025, the European Commission will adopt the award decision. The European Climate, Infrastructure and Environment Executive Agency (CINEA) is starting the preparation of the grant agreements with the successful projects.

    Due to the exhaustion of funds, the third cut-off will be cancelled. The Commission will now assess the potential reflows and subsequently prepare a new work programme and call for proposals in the coming months. 

    Background

    The projects have been selected under the second cut-off of the 2024-2025 AFIF call which closed on 11 June 2025. The total awarded grant for these projects is €600 million: €505 million under the General envelope and €95 million under the Cohesion envelope. 

    A total budget of €1 billion was available under this Call: €780 million under the General envelope and €220 million under the Cohesion envelope. This call supports the objectives for publicly accessible electric recharging pools and hydrogen refuelling stations across the EU’s main transport corridors and hubs as set out in the Regulation for the deployment of alternative fuels infrastructure (AFIR), in the ReFuelEU aviation regulation and in the FuelEU maritime regulation. 

    AFIF also aims to improve alternative fuels infrastructure in ports by investing strongly in OPS. This facilitates the transition to renewable and low-carbon fuels by ships, which is also a main priority outlined in the Sustainable Transport Investment Plan. Regarding heavy-duty vehicle charging infrastructure, the Automotive Action Plan encourages the adoption of zero-emission vehicles by speeding up the deployment of necessary infrastructure. 

    For more information

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  • Hyundai Teases CRATER Concept Global Debut Ahead of AutoMobility LA 2025

    Hyundai Teases CRATER Concept Global Debut Ahead of AutoMobility LA 2025

    The CRATER Concept will be viewable throughout AutoMobility LA 2025 media days, as well as Los Angeles Auto Show public days from Fri., Nov. 21 – Sun., Nov. 30. In addition, the vehicle’s global debut press conference will be livestreamed around the world. The broadcast can be viewed beginning at 9:45 a.m. PT. Tune in to see the reveal of Hyundai’s bold new off-road concept vehicle.

    CRATER Concept is a compact off-road SUV show vehicle that embodies capability and toughness. It is a design exploration that captures the spirit of adventure. Inspired by extreme environments, the CRATER Concept was conceived at Hyundai America Technical Center (HATCI) in Irvine, Calif. and has been crafted to amplify the same spirit and robustness found in Hyundai’s XRT production vehicles, including the IONIQ 5 XRT , SANTA CRUZ XRT , and the new PALISADE XRT PRO .

    Hyundai Motor America

    Hyundai Motor America offers U.S. consumers a technology-rich lineup of cars, SUVs, and electrified vehicles, while supporting Hyundai Motor Company’s Progress for Humanity vision. Hyundai has significant operations in the U.S., including its North American headquarters in California, the Hyundai Motor Manufacturing Alabama assembly plant, the all-new Hyundai Motor Group Metaplant America, and several cutting-edge R&D facilities. These operations, combined with those of Hyundai’s 850 independent dealers, contribute $20.1 billion annually and 190,000 jobs to the U.S. economy, according to a published economic impact report. For more information, visit www.hyundainews.com .

    Hyundai Motor America on Twitter | YouTube | Facebook | Instagram | LinkedIn | TikTok

    SOURCE Hyundai Motor America


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  • GWR train fitted with F1 tech for two-month superfast wifi trial | Rail industry

    GWR train fitted with F1 tech for two-month superfast wifi trial | Rail industry

    Train wifi in the UK, long a source of frustration for passengers, is about to get radically faster – for a lucky few at least.

    A two-month trial has begun on one Great Western Railway (GWR) train, fitted with technology from Formula One that switches between the signals from 5G masts to low Earth-orbit satellites to provide almost seamless, superfast wifi.

    For now, only one of GWR’s 57 intercity express trains will have a connection good enough to deliver a Netflix series to the seat. However, a successful trial and the promise of lower costs could spell a wider rollout to the rest of the mainline railway by 2030.

    On a test run from London Paddington to Newbury and back, the Guardian found the wifi fast and reliable enough to video call editors at the office, catch up on old Match of the Days on iPlayer and listen to songs on YouTube at the same time, with only occasional blips and pixelation.

    Download speeds reached more than 120 megabytes a second, faster than many homes.

    Speaking at Paddington at the launch of the trial, the rail minister, Peter Hendy, said: “Passenger experience is top of our agenda – and 21st-century experience ought to be seamless fast wifi … which will make the time spent travelling by train even more valuable.”

    He said the trial would complement government investment in improving mobile connectivity, with another £41m set aside for train wifi and low-orbit satellite connections, announced in June’s spending review. The Department for Transport is funding work to eliminate mobile signal black spots in rail tunnels and upgrading 5G infrastructure at stations on GWR routes.

    Lord Hendy said the new state-owned Great British Railways would aspire to fast wifi across the entire railway, but added: “The real question is how quickly and how cheaply it can be rolled out.”

    Hendy said it could be “a real productivity benefit for the whole country, hopefully at a modest cost”.

    He said the department would be awaiting the results of the trial, but its advocates claim the new system could be installed relatively quickly and cheaply without requiring extra infrastructure on the railway. The previous government was considering scrapping free wifi on trains because of the unreliability and cost.

    Nick Fry, the chair of Motion Applied, a tech company spun out of the McLaren racing division, said the pilot would demonstrate the technology was ready. The UK-made tech, pioneered in F1, combines “several pizza-sized boxes” and antennae attached to the roof of the train, allowing it to connect and switch between the best available network, from wifi to 5G to satellite, he said. “It’s very fast with fewer dropouts.”

    The system is also being rolled out on Deutsche Bahn services in Germany and on Brightline and Amtrak trains in the US.

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    “We look forward to providing rail passengers with the same service we provide for Lando Norris and Lewis Hamilton,” Fry added.

    Part of the trial will be to track passenger behaviour to see how much satellite data would be required if free, fast wifi was available for streaming.

    The £300k cost is being funded by Peninsula Transport, a body combining Devon, Cornwall, Plymouth, Somerset and Torbay, with better connectivity seen as a critical investment for parts of England where mobile coverage is patchy.

    Businesses have welcomed the trial. Andy Jasper, the chief executive of the Eden Project in Cornwall, said GWR trains were his “travelling office, and a bloodstream between Cornwall and London – new wifi is going to be the oxygen that keeps everything pumping”.

    Jasper said he was used to having to time conversations onboard for when he knew the wifi would work, such as a quick 10-minute Teams meeting in Plymouth. “Reliable wifi puts your mind at ease – it turns the journey into a prime opportunity to get things done.”

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  • Treasurys, Dollar Rangebound Amid Cloudy Fed Outlook – The Wall Street Journal

    1. Treasurys, Dollar Rangebound Amid Cloudy Fed Outlook  The Wall Street Journal
    2. Treasury yields inch lower as investors anticipate delayed economic data  CNBC
    3. US 10-Year Yield Holds Advance  TradingView
    4. Treasuries, Dollar Rangebound Amid Cloudy Fed Outlook  Barron’s
    5. Bond Traders Eye Make-or-Break Data to Chart Fed’s Next Move  Yahoo Finance

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  • Jeff Bezos reportedly launches new AI startup with himself as CEO | Technology

    Jeff Bezos reportedly launches new AI startup with himself as CEO | Technology

    After stepping down as Amazon’s CEO four years ago, Jeff Bezos, the billionaire founder and former chief executive of the online shopping company, is going to be a CEO again. This time, Bezos has appointed himself co-CEO of an AI startup called Project Prometheus, the New York Times reported, citing anonymous sources.

    The startup, which will focus on developing AI for engineering and manufacturing in various fields, has already received $6.2bn in funding – more than many companies are able to raise in their lifetimes. Leading the company alongside Bezos is his co-founder and co-CEO Vik Bajaj, a celebrity tech executive in his own right. Bajaj is a physicist and chemist best known for his work at Google’s moonshot factory, X, where he founded the health startup Verily.

    It’s unclear how long the company has existed, but Project Prometheus has already hired 100 employees, poaching several from firms like OpenAI, DeepMind and Meta, according to the Times. Little else is known about the project, as Bezos did not disclose where the company will be based or how its technology might function. The world’s third-richest person has been closely involved at his aerospace company Blue Origin for several years as its founder and sole shareholder, but becoming a CEO again will be the first formal role Bezos has taken since stepping down from Amazon.

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    Bezos and Bajaj join a crowded AI marketplace where billions of dollars are being poured into competitors like OpenAI and billions more are being spent to support the rapid development of AI models. More experts are beginning to question the financial sustainability of the AI industry, though. Michael Burry, best known for accurately predicting the 2008 housing crisis, recently invested $1bn in bets that Palantir and Nvidia shares will fall just days after he accused some of the big tech firms of using accounting tricks to “artificially boost earnings”.

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  • Nvidia’s Jensen Huang says ‘to be a CEO is a lifetime of sacrifice,’ but his parents prepared him for the ‘pain and suffering’ of leadership

    Nvidia’s Jensen Huang says ‘to be a CEO is a lifetime of sacrifice,’ but his parents prepared him for the ‘pain and suffering’ of leadership

    Nvidia CEO Jensen Huang says that leading a company like chipmaker Nvidia is a privilege but it also requires an individual to sacrifice their life to be of service to the business and its employees.

    Huang, worth $165 billion according to the Bloomberg Billionaires Index, recently gave a talk to students at the University of Cambridge, in which he described how his parents’ pursuit of success in the U.S. laid the foundation for his work ethic in building his own company. Huang, along with two friends, founded Nvidia in 1993 and over the past two decades has grown the business to a market cap of more than $4.6 trillion.

    He launched Nvidia from a Denny’s dining booth without a business plan, he said, and had to learn his leadership and management skills on the job. After all, Nvidia is the first and only business he has ever led.

    His mantra for success has been simple: “Don’t get bored and don’t get fired,” he said at the event earlier this month.

    While that sounds straightforward enough, Huang also warned a life of extreme success in a highly competitive industry like tech is not without its drawbacks.

    When discussing why he, as opposed to his engineer co-founders Chris Malachowsky and Curtis Priem, landed the top job, Huang said it was because “they didn’t want the job.”

    “In retrospect, I could have been smarter myself, and to be CEO is a lifetime of sacrifice,” Huang told his audience. “Most people think that it’s about leading and being in command and being on top. None of that is true. You’re in service of the company. You’re creating conditions for other people to do their life’s work, you’re inspiring through example. Most of the examples are making difficult decisions during very difficult times, it’s mostly about sacrifiice.”

    “It’s about strategy, and strategy … is not just about choosing what to do, it’s about choosing what not to do, which is sacrifice, and the determination, the conviction, the pain and suffering that goes along with overcoming obstacles, that’s all sacrifice.”

    Huang has been open about his unrivalled work ethic and the commitment he expects from his employees. Previously, the 62-year-old CEO said he works from the moment he wakes up until the moment he goes to sleep, adding in an interview with Stripe’s CEOPatrick Collison last year that he can’t even watch a movie without thinking about his company.

    In a 60 Minutes interview in 2024 he was asked whether “demanding,” “perfectionist,” and “not easy to work for,” were accurate descriptions of him, Huang said they fit him “perfectly,” explaining: “It should be like that. If you want to do extraordinary things, it shouldn’t be easy.”

    A family trait

    The Taiwanese-American entrepreneur attributed his commitment and determination to his parents: His father, determined to see his children grow up in America; and his mother, teaching her children English despite the fact she didn’t speak the language herself.

    “My parents wanted us to pursue the American Dream,” Huang said. “They didn’t have very much, they were quite modest, and moving to the United States was quite difficult for us in 1973, but somehow we made our way through it. I think the life of struggle, endeavour, nothing for granted, having to earn anything, I think was good CEO training.”

    Huang went on to study at Oregon State and Stanford University, crediting his parents with instilling a belief in him that he could achieve. Wearing his usual leather jacket, Huang added his mother had insisted he was “special,” explaining: “Often times, if people tell you that you’re better, greater, more capable than you are, you might live up to that expectation. It reminds us to do the same with our companies, it reminds us to do the same with each other.”

    “[My mother] left me with an impression that nothing could be that hard, to this day, and people have seen me adapt.”

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