Category: 8. Health

  • Neuro Balance Therapy Update: Strongest Non-Clinical Reflex

    Neuro Balance Therapy Update: Strongest Non-Clinical Reflex

    Chicago, July 03, 2025 (GLOBE NEWSWIRE) —

    Section 1 – Introduction: Reframing Fall Risk in Aging Populations

    Across the United States, fall-related injuries continue to be a top health concern among older adults. Data from the CDC reveals that one in four Americans aged 65+ experiences a fall each year, with over 800,000 requiring hospitalization. While often attributed to aging, recent biomechanical research highlights deeper underlying causes, including diminished sensory feedback from the feet—an overlooked but significant contributor to instability. With the search for safe, sustainable interventions intensifying, non-invasive options that align with lifestyle accessibility and neuroscience are drawing increased interest.

    The emergence of nerve-centric balance theories has led to a shift in how wellness providers approach fall prevention. Rather than relying solely on strength-based rehabilitation, newer approaches focus on restoring proprioceptive communication—how the body senses ground contact and responds reflexively to prevent imbalance. These trends are shaping consumer expectations around what it means to stay mobile, strong, and independent while aging. Neuro Balance Therapy is among the programs aiming to meet this demand with targeted, at-home methods based on new understandings of nerve-muscle coordination.

    To access the full program details and review the educational material, visit the official platform.

    Section 2 – Neuro Balance Therapy Program Update: Evolution of a Non-Clinical Stability Protocol

    In alignment with ongoing demand for at-home balance strategies, Neuro Balance Therapy has released a 2025 update to its protocol, reinforcing its educational foundation in proprioceptive activation and nerve-response science. Developed by Certified Balance Specialist Chris Wilson, the program continues to provide structured instruction centered around the stimulation of the deep peroneal nerve—a sensory-motor pathway shown in academic studies to play a critical role in foot reflexes and muscular coordination.

    Unlike strength-based fitness plans or conventional physiotherapy sessions, Neuro Balance Therapy centers on a simple, time-efficient daily ritual that requires no gym equipment, medication, or clinical oversight. This protocol uses a proprietary “nerve wake-up” ball designed to apply patterned pressure across the plantar surface of the foot to help retrain dormant nerve endings that contribute to instability.

    The 2025 update maintains its original DVD format with guided video sequences segmented by progression level—beginner, intermediate, and advanced—while also offering a digital option for increased accessibility. The program is particularly tailored for adults over 60 who may experience heightened fear of falling, previous fall incidents, or early signs of reduced mobility.

    Program developer Wilson highlights that this expanded reach reflects an effort to ensure that aging adults have a reliable, educational tool to build confidence in movement. Neuro Balance Therapy remains grounded in a non-diagnostic framework and is presented as a supportive lifestyle enhancement based on emerging research in neuromechanics and gerontology.

    Section 3 – Trend Analysis: Public Concerns, Fall Anxiety, and Rising Interest in Home-Based Prevention

    Fall prevention has become one of the most searched-for health concerns among adults over 60, particularly those living independently or recovering from injury. A growing body of online discussions reveals a pattern of fear, hesitation, and uncertainty among older individuals—especially those who have experienced falls in the past or live with limited mobility. This trend is reflected not only in medical safety forums and caregiver blogs but also in keyword search data indicating a spike in interest around terms like “fall-proof exercises,” “senior balance support,” and “home remedies for stability.”

    As mobility-related anxiety grows, consumers are increasingly looking for evidence-based yet low-barrier solutions that can be adopted from home. Rather than focusing solely on strength or endurance, public attention has turned to neuromuscular control—specifically the role of reflexive foot response in preventing unintentional falls. Individuals researching fall risk reduction now frequently encounter terms like “proprioception,” “nerve activation,” and “sensorimotor training” as part of this broader narrative shift.

    Within this climate of heightened concern and education-seeking behavior, Neuro Balance Therapy has attracted attention for its practical alignment with these needs. Its core premise—that reawakening a dormant foot nerve can reengage natural reflexes and increase stability—reflects the growing interest in nervous system-focused approaches that move beyond traditional exercise or strength-building routines. While not positioned as a replacement for clinical care, the protocol meets a gap in the market where education, self-reliance, and routine movement rituals converge.

    Further information, including transparency disclosures and instructional methodology, is available through the authorized distribution channel.

    Section 4 – Spotlight on Proprioceptive Tools and Neuromuscular Activation

    At the core of the Neuro Balance Therapy protocol is a targeted tool: a specialized spiked ball engineered to stimulate sensory receptors along the sole of the foot. While deceptively simple in appearance, the design is based on emerging studies around tactile feedback and the role of deep nerves in foot stability. The tool is used in a brief 10-second morning ritual to activate what researchers identify as the deep peroneal nerve, which plays a critical role in reflexively engaging foot and lower leg muscles when movement begins or imbalance occurs.

    The textured surface of the ball is constructed with hardened crystalline polymers—chosen for their ability to provide a consistent, non-compressive stimulus during rolling motions. This form of physical input has been shown in third-party studies to support the re-engagement of sensory-motor pathways that often degrade with age, shoe use, or injury recovery. Rather than functioning as an exercise device, the tool acts as a proprioceptive primer—a method to help the body regain contact awareness with the ground and reestablish neuromuscular timing.

    This approach draws on insights from evolutionary biomechanics and comparative anthropology, such as those observed in populations accustomed to barefoot mobility. Researchers from Harvard and other institutions have noted that such individuals tend to exhibit stronger foot-ground sensory feedback and lower fall risk later in life—attributed not to strength but to continuous nerve engagement over time.

    While Neuro Balance Therapy does not claim to treat or diagnose any condition, the inclusion of this tactile tool is aligned with broader wellness trends that seek to improve functional confidence through non-invasive, neuro-supportive interventions. The emphasis on activating rather than strengthening mirrors a shift in how consumers and wellness educators approach stability challenges—one rooted in nervous system health rather than musculoskeletal strain.

    Section 5 – User Journey Narrative and Market Reception

    Ongoing online dialogue reveals a clear and consistent theme among older adults concerned with fall-related limitations: the emotional toll of instability often surpasses the physical impact. Reports across support forums and aging wellness platforms reflect a growing frustration with traditional mobility solutions that feel either inaccessible or incomplete. Many express the desire to feel “safe in their own homes” or to reclaim confidence performing routine tasks without the looming anxiety of a fall.

    Within this discourse, Neuro Balance Therapy has emerged as a topic of interest, particularly among individuals seeking alternatives to large-scale physical therapy programs or invasive medical options. While individual testimonials are not cited in public releases for compliance reasons, general sentiment within the community has centered on the appeal of the program’s simplicity, home-based accessibility, and the perceived novelty of targeting a sensory nerve often overlooked in standard fall prevention plans.

    The narrative tone of market discussions is often one of cautious optimism. Some describe past experiences with balance training programs that required gym access, heavy equipment, or high mobility thresholds—barriers that left many behind. In contrast, Neuro Balance Therapy is often mentioned as an entry-point protocol: a minimal-commitment routine that offers a sense of daily empowerment and a framework for understanding how balance and stability can be proactively supported without intensive oversight.

    This emotional connection—between fear, autonomy, and confidence—continues to shape the public reception of fall-prevention solutions. Neuro Balance Therapy’s alignment with those concerns, coupled with its non-intimidating delivery format, has contributed to its sustained visibility among aging communities, wellness bloggers, and caregiving resource hubs.

    Section 6 – Availability and Transparency Statement

    The complete Neuro Balance Therapy program is currently accessible through the brand’s official platform, where consumers can review educational materials and explore the science-backed methodology behind the protocol. The program is offered in both physical and digital formats to accommodate varying technology preferences and household setups. Each version includes access to guided instructional content and the proprietary nerve activation tool central to the protocol’s design.

    All educational content within the program is intended strictly for informational purposes and is presented as a non-clinical, home-based resource. Neuro Balance Therapy does not diagnose or treat medical conditions, and individuals are advised to consult with their healthcare provider before beginning any new wellness regimen. Program components are designed to complement, not replace, broader wellness or physical rehabilitation efforts and are delivered in alignment with current best practices in consumer safety and instructional clarity.

    Further product details, background information, and transparency disclosures can be found on the official program website.

    Information about program structure, availability, and research alignment can be found at the official access site.

    Section 7 – Final Observations and Industry Context

    The current landscape of fall-prevention solutions reveals an evolving market shaped by demographic trends and wellness consumer expectations. As the population over 60 continues to expand, so does the need for preventative strategies that prioritize accessibility, non-invasiveness, and autonomy. With falls now recognized by public health institutions as both a clinical and lifestyle issue, there is increased scrutiny on programs that promise safety without sustainable, evidence-informed frameworks.

    Neuro Balance Therapy enters this environment with a measured approach—framing fall prevention not as a fitness goal, but as a daily practice of neurosensory engagement. Its emphasis on proprioceptive restoration, combined with an at-home format, reflects growing demand for solutions that bridge the gap between clinical rehabilitation and consumer-directed wellness. As the industry moves toward greater personalization in health routines, protocols that integrate functional simplicity with research-inspired insights are likely to gain traction.

    This update also underscores a wider trend: the reframing of mobility and balance not as age-related inevitabilities, but as skillsets that can be reinforced through nerve and muscle coordination. While more longitudinal research is warranted, early public interest in such targeted tools suggests a shift away from generalized exercise programs toward precision-oriented interventions.

    As balance science continues to develop, Neuro Balance Therapy positions itself as part of the broader movement redefining how older adults interact with their own mobility—through agency, awareness, and the reawakening of long-dormant neuromechanical responses.

    Section 8 – Public Commentary Theme Summary: Risk Awareness, Independence, and Program Accessibility

    Public conversations surrounding fall prevention continue to reveal a wide spectrum of experiences and emotions—ranging from fear and frustration to empowerment and cautious hope. A recurring discussion point in online forums and wellness communities is the fear of losing independence due to instability or past fall incidents. Many express concern over the limitations of traditional mobility programs, which often require clinical oversight, frequent appointments, or physical exertion that can feel overwhelming for individuals with existing discomfort or restricted movement.

    Some have noted the appeal of Neuro Balance Therapy for its non-strenuous routine and minimal space requirements. The fact that the program can be done while seated—without the need for specialized shoes, physical therapy appointments, or weight-bearing movement—has drawn interest among those recovering from injury or living alone.

    Skepticism also surfaces in certain segments of the discussion. A common question centers on whether a simple nerve-activation ritual could truly make a difference, particularly when contrasted with more robust physical therapy approaches. Others express concern over the proliferation of at-home solutions that appear promising but offer limited scientific transparency. However, the continued referencing of supporting studies on proprioception and deep nerve reflexes helps validate consumer interest and aligns with a trend toward self-education in aging wellness.

    Overall, public commentary reflects a desire for straightforward, low-barrier entry points into fall-prevention that emphasize autonomy, emotional reassurance, and ease of use. Neuro Balance Therapy’s positioning within that dialogue—especially as it avoids over-promising outcomes—has enabled it to retain credibility while encouraging broader conversations about sensory-motor awareness as a foundation for lifelong mobility.

    About the Company

    Neuro Balance Therapy is a wellness education program founded with the goal of supporting older adults in regaining stability, confidence, and ease of movement through non-clinical, home-based strategies. Established by Certified Balance Specialist Chris Wilson, the program draws on over a decade of experience in balance instruction and proprioceptive training.

    Rooted in research-backed methods, Neuro Balance Therapy offers instructional tools designed to help activate underutilized sensory nerves responsible for lower-body coordination. The company’s approach reflects a broader commitment to empowering individuals through accessible routines that promote physical self-reliance. Neuro Balance Therapy does not provide treatment or diagnostic services and encourages all users to consult their healthcare provider before beginning any new movement or balance routine.

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  • Strongest Non-Clinical Reflex Routine for Seniors Concerned About Instability

    Strongest Non-Clinical Reflex Routine for Seniors Concerned About Instability

    Neuro-Balance Therapy

    New 2025 At-Home Protocol Uses OTC Nerve Activation Tool to Reinforce Stability, Restore Confidence, and Help Reduce Fall Risk in Adults 60+

    Chicago, July 03, 2025 (GLOBE NEWSWIRE) —

    Neuro Balance Therapy Update Strongest Non-Clinical Reflex Routine for Seniors Concerned About Instability
    Neuro Balance Therapy Update Strongest Non-Clinical Reflex Routine for Seniors Concerned About Instability

    Section 1 – Introduction: Reframing Fall Risk in Aging Populations

    Across the United States, fall-related injuries continue to be a top health concern among older adults. Data from the CDC reveals that one in four Americans aged 65+ experiences a fall each year, with over 800,000 requiring hospitalization. While often attributed to aging, recent biomechanical research highlights deeper underlying causes, including diminished sensory feedback from the feet—an overlooked but significant contributor to instability. With the search for safe, sustainable interventions intensifying, non-invasive options that align with lifestyle accessibility and neuroscience are drawing increased interest.

    The emergence of nerve-centric balance theories has led to a shift in how wellness providers approach fall prevention. Rather than relying solely on strength-based rehabilitation, newer approaches focus on restoring proprioceptive communication—how the body senses ground contact and responds reflexively to prevent imbalance. These trends are shaping consumer expectations around what it means to stay mobile, strong, and independent while aging. Neuro Balance Therapy is among the programs aiming to meet this demand with targeted, at-home methods based on new understandings of nerve-muscle coordination.

    To access the full program details and review the educational material, visit the official platform.

    Section 2 – Neuro Balance Therapy Program Update: Evolution of a Non-Clinical Stability Protocol

    In alignment with ongoing demand for at-home balance strategies, Neuro Balance Therapy has released a 2025 update to its protocol, reinforcing its educational foundation in proprioceptive activation and nerve-response science. Developed by Certified Balance Specialist Chris Wilson, the program continues to provide structured instruction centered around the stimulation of the deep peroneal nerve—a sensory-motor pathway shown in academic studies to play a critical role in foot reflexes and muscular coordination.

    Unlike strength-based fitness plans or conventional physiotherapy sessions, Neuro Balance Therapy centers on a simple, time-efficient daily ritual that requires no gym equipment, medication, or clinical oversight. This protocol uses a proprietary “nerve wake-up” ball designed to apply patterned pressure across the plantar surface of the foot to help retrain dormant nerve endings that contribute to instability.

    The 2025 update maintains its original DVD format with guided video sequences segmented by progression level—beginner, intermediate, and advanced—while also offering a digital option for increased accessibility. The program is particularly tailored for adults over 60 who may experience heightened fear of falling, previous fall incidents, or early signs of reduced mobility.

    Program developer Wilson highlights that this expanded reach reflects an effort to ensure that aging adults have a reliable, educational tool to build confidence in movement. Neuro Balance Therapy remains grounded in a non-diagnostic framework and is presented as a supportive lifestyle enhancement based on emerging research in neuromechanics and gerontology.

    Section 3 – Trend Analysis: Public Concerns, Fall Anxiety, and Rising Interest in Home-Based Prevention

    Fall prevention has become one of the most searched-for health concerns among adults over 60, particularly those living independently or recovering from injury. A growing body of online discussions reveals a pattern of fear, hesitation, and uncertainty among older individuals—especially those who have experienced falls in the past or live with limited mobility. This trend is reflected not only in medical safety forums and caregiver blogs but also in keyword search data indicating a spike in interest around terms like “fall-proof exercises,” “senior balance support,” and “home remedies for stability.”

    As mobility-related anxiety grows, consumers are increasingly looking for evidence-based yet low-barrier solutions that can be adopted from home. Rather than focusing solely on strength or endurance, public attention has turned to neuromuscular control—specifically the role of reflexive foot response in preventing unintentional falls. Individuals researching fall risk reduction now frequently encounter terms like “proprioception,” “nerve activation,” and “sensorimotor training” as part of this broader narrative shift.

    Within this climate of heightened concern and education-seeking behavior, Neuro Balance Therapy has attracted attention for its practical alignment with these needs. Its core premise—that reawakening a dormant foot nerve can reengage natural reflexes and increase stability—reflects the growing interest in nervous system-focused approaches that move beyond traditional exercise or strength-building routines. While not positioned as a replacement for clinical care, the protocol meets a gap in the market where education, self-reliance, and routine movement rituals converge.

    Further information, including transparency disclosures and instructional methodology, is available through the authorized distribution channel.

    Section 4 – Spotlight on Proprioceptive Tools and Neuromuscular Activation

    At the core of the Neuro Balance Therapy protocol is a targeted tool: a specialized spiked ball engineered to stimulate sensory receptors along the sole of the foot. While deceptively simple in appearance, the design is based on emerging studies around tactile feedback and the role of deep nerves in foot stability. The tool is used in a brief 10-second morning ritual to activate what researchers identify as the deep peroneal nerve, which plays a critical role in reflexively engaging foot and lower leg muscles when movement begins or imbalance occurs.

    The textured surface of the ball is constructed with hardened crystalline polymers—chosen for their ability to provide a consistent, non-compressive stimulus during rolling motions. This form of physical input has been shown in third-party studies to support the re-engagement of sensory-motor pathways that often degrade with age, shoe use, or injury recovery. Rather than functioning as an exercise device, the tool acts as a proprioceptive primer—a method to help the body regain contact awareness with the ground and reestablish neuromuscular timing.

    This approach draws on insights from evolutionary biomechanics and comparative anthropology, such as those observed in populations accustomed to barefoot mobility. Researchers from Harvard and other institutions have noted that such individuals tend to exhibit stronger foot-ground sensory feedback and lower fall risk later in life—attributed not to strength but to continuous nerve engagement over time.

    While Neuro Balance Therapy does not claim to treat or diagnose any condition, the inclusion of this tactile tool is aligned with broader wellness trends that seek to improve functional confidence through non-invasive, neuro-supportive interventions. The emphasis on activating rather than strengthening mirrors a shift in how consumers and wellness educators approach stability challenges—one rooted in nervous system health rather than musculoskeletal strain.

    Section 5 – User Journey Narrative and Market Reception

    Ongoing online dialogue reveals a clear and consistent theme among older adults concerned with fall-related limitations: the emotional toll of instability often surpasses the physical impact. Reports across support forums and aging wellness platforms reflect a growing frustration with traditional mobility solutions that feel either inaccessible or incomplete. Many express the desire to feel “safe in their own homes” or to reclaim confidence performing routine tasks without the looming anxiety of a fall.

    Within this discourse, Neuro Balance Therapy has emerged as a topic of interest, particularly among individuals seeking alternatives to large-scale physical therapy programs or invasive medical options. While individual testimonials are not cited in public releases for compliance reasons, general sentiment within the community has centered on the appeal of the program’s simplicity, home-based accessibility, and the perceived novelty of targeting a sensory nerve often overlooked in standard fall prevention plans.

    The narrative tone of market discussions is often one of cautious optimism. Some describe past experiences with balance training programs that required gym access, heavy equipment, or high mobility thresholds—barriers that left many behind. In contrast, Neuro Balance Therapy is often mentioned as an entry-point protocol: a minimal-commitment routine that offers a sense of daily empowerment and a framework for understanding how balance and stability can be proactively supported without intensive oversight.

    This emotional connection—between fear, autonomy, and confidence—continues to shape the public reception of fall-prevention solutions. Neuro Balance Therapy’s alignment with those concerns, coupled with its non-intimidating delivery format, has contributed to its sustained visibility among aging communities, wellness bloggers, and caregiving resource hubs.

    Section 6 – Availability and Transparency Statement

    The complete Neuro Balance Therapy program is currently accessible through the brand’s official platform, where consumers can review educational materials and explore the science-backed methodology behind the protocol. The program is offered in both physical and digital formats to accommodate varying technology preferences and household setups. Each version includes access to guided instructional content and the proprietary nerve activation tool central to the protocol’s design.

    All educational content within the program is intended strictly for informational purposes and is presented as a non-clinical, home-based resource. Neuro Balance Therapy does not diagnose or treat medical conditions, and individuals are advised to consult with their healthcare provider before beginning any new wellness regimen. Program components are designed to complement, not replace, broader wellness or physical rehabilitation efforts and are delivered in alignment with current best practices in consumer safety and instructional clarity.

    Further product details, background information, and transparency disclosures can be found on the official program website.

    Information about program structure, availability, and research alignment can be found at the official access site.

    Section 7 – Final Observations and Industry Context

    The current landscape of fall-prevention solutions reveals an evolving market shaped by demographic trends and wellness consumer expectations. As the population over 60 continues to expand, so does the need for preventative strategies that prioritize accessibility, non-invasiveness, and autonomy. With falls now recognized by public health institutions as both a clinical and lifestyle issue, there is increased scrutiny on programs that promise safety without sustainable, evidence-informed frameworks.

    Neuro Balance Therapy enters this environment with a measured approach—framing fall prevention not as a fitness goal, but as a daily practice of neurosensory engagement. Its emphasis on proprioceptive restoration, combined with an at-home format, reflects growing demand for solutions that bridge the gap between clinical rehabilitation and consumer-directed wellness. As the industry moves toward greater personalization in health routines, protocols that integrate functional simplicity with research-inspired insights are likely to gain traction.

    This update also underscores a wider trend: the reframing of mobility and balance not as age-related inevitabilities, but as skillsets that can be reinforced through nerve and muscle coordination. While more longitudinal research is warranted, early public interest in such targeted tools suggests a shift away from generalized exercise programs toward precision-oriented interventions.

    As balance science continues to develop, Neuro Balance Therapy positions itself as part of the broader movement redefining how older adults interact with their own mobility—through agency, awareness, and the reawakening of long-dormant neuromechanical responses.

    Section 8 – Public Commentary Theme Summary: Risk Awareness, Independence, and Program Accessibility

    Public conversations surrounding fall prevention continue to reveal a wide spectrum of experiences and emotions—ranging from fear and frustration to empowerment and cautious hope. A recurring discussion point in online forums and wellness communities is the fear of losing independence due to instability or past fall incidents. Many express concern over the limitations of traditional mobility programs, which often require clinical oversight, frequent appointments, or physical exertion that can feel overwhelming for individuals with existing discomfort or restricted movement.

    Some have noted the appeal of Neuro Balance Therapy for its non-strenuous routine and minimal space requirements. The fact that the program can be done while seated—without the need for specialized shoes, physical therapy appointments, or weight-bearing movement—has drawn interest among those recovering from injury or living alone.

    Skepticism also surfaces in certain segments of the discussion. A common question centers on whether a simple nerve-activation ritual could truly make a difference, particularly when contrasted with more robust physical therapy approaches. Others express concern over the proliferation of at-home solutions that appear promising but offer limited scientific transparency. However, the continued referencing of supporting studies on proprioception and deep nerve reflexes helps validate consumer interest and aligns with a trend toward self-education in aging wellness.

    Overall, public commentary reflects a desire for straightforward, low-barrier entry points into fall-prevention that emphasize autonomy, emotional reassurance, and ease of use. Neuro Balance Therapy’s positioning within that dialogue—especially as it avoids over-promising outcomes—has enabled it to retain credibility while encouraging broader conversations about sensory-motor awareness as a foundation for lifelong mobility.

    About the Company

    Neuro Balance Therapy is a wellness education program founded with the goal of supporting older adults in regaining stability, confidence, and ease of movement through non-clinical, home-based strategies. Established by Certified Balance Specialist Chris Wilson, the program draws on over a decade of experience in balance instruction and proprioceptive training.

    Rooted in research-backed methods, Neuro Balance Therapy offers instructional tools designed to help activate underutilized sensory nerves responsible for lower-body coordination. The company’s approach reflects a broader commitment to empowering individuals through accessible routines that promote physical self-reliance. Neuro Balance Therapy does not provide treatment or diagnostic services and encourages all users to consult their healthcare provider before beginning any new movement or balance routine.

    Contact

    CONTACT: Email: info@neurobalancetherapy.org

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  • RFK Jr.’s warnings about sperm counts fuel doomsday claims about male fertility

    RFK Jr.’s warnings about sperm counts fuel doomsday claims about male fertility

    It’s not uncommon for Health and Human Services Secretary Robert F. Kennedy Jr. to mention sperm counts when he makes a public appearance.

    In recent television interviews, political speeches and congressional hearings, Kennedy has repeatedly claimed that teenage boys today have half the sperm that men in their 60s do — a stat that’s not exactly accurate. Kennedy has cited the talking point as evidence of a broader health crisis in the U.S.

    “We have fertility rates that are just spiraling. A teenager today, an American teenager, has less testosterone than a 68-year-old man. Sperm counts are down 50%,” he told Fox News’ Jesse Watters in April, adding: “It’s an existential problem.”

    Contrary to Kennedy’s claims, sperm counts decline with age, so young men have much higher counts than older men. And data about sperm counts in teen boys largely does not exist.

    Some researchers contend that men’s overall sperm counts are lower than they were generations ago, based mostly on two papers published in the last decade. Others say there’s no convincing evidence of the trend. And many agree that even if sperm counts are declining, it does not amount to a full-blown fertility crisis.

    “This is a very contentious issue in our field, and for every paper that you find that suggests a decline and raises an alarm for this issue, there’s another paper that says that the numbers aren’t changing, and that there’s no cause for concern,” said Dr. Scott Lundy, a reproductive urologist at the Cleveland Clinic.

    Andrew Nixon, an HHS spokesperson, said Kennedy is “sounding the alarm on a public health issue others are too timid, or too politically cautious, to confront.”

    The secretary’s warning feeds on a burgeoning narrative that men today face a fundamental threat to their fertility. Similar claims have been spread by various wellness influencers, tech startups and young men on social media. Young men concerned about a decline in virility have opted to freeze their sperm, abstain from sex or undergo testosterone replacement therapy. A 2022 study found that “semen retention” was the most popular men’s health subject on TikTok and Instagram.

    Meanwhile, adherents of the “pro-natalist” movement have argued that more families should be having children to compensate for a decline in fertility and birth rates in the U.S. The most prominent figure among them, Elon Musk, has cited the declining birth rate as an omen of humanity’s collapse.

    Researchers who study male fertility say the reality is far more complicated and little cause for panic. Fertility and birth rates in the U.S. are declining, in part, because people are choosing to have fewer children or delaying having kids until later in life. Though some men do struggle to have kids, in many cases the issue can be corrected through medical interventions or lifestyle changes.

    In 1993, scientist Louis Guillette shocked Congress when he testified at a hearing that “every man sitting in this room today is half the man his grandfather was.”

    Guillette was referring to a generational decline in sperm count. A year before his testimony, a review of papers published from 1938 to 1991 determined that the average sperm count had fallen around 50%.

    But many researchers have since found flaws in the review — among them, that it included relatively little data from the first few decades of the analysis, the men in the studies were evaluated using different methods and the data analysis did not account for the fact that many men’s sperm counts fall within a lower range.

    “The paper was widely, wildly cited,” but “the statistics were not solid,” said Dolores Lamb, who researches male infertility at Children’s Mercy Kansas City.

    In a follow-up review of studies published from 1992 to 2013, eight studies showed a decline in semen quality, 21 showed no change or an increase, and six showed ambiguous or conflicting results. Based on that, Lamb said, “the preponderance of the data suggests that there was no decline.”

    In 2021, reproductive epidemiologist Shanna Swan reignited the debate with her book “Count Down,” which warned of falling sperm counts “imperiling the future of the human race.”

    A paper Swan and her co-authors published in 2017 determined that from 1973 to 2011, sperm counts declined by 52% in North America, Europe, Australia and New Zealand. A follow-up analysis in 2022 showed a similar trend worldwide. In an interview with The Guardian, Swan said her work implied that the median sperm count could reach zero by 2045.

    The research was picked up by men’s rights groups, which pointed to it as evidence that men were losing their masculinity. It even inspired a viral publicity stunt to raise awareness about a possible future where people couldn’t reproduce: A crowd gathered to watch sperm cells race under a microscope.

    HHS’ Nixon said the 2017 and 2022 papers support Kennedy’s claims about declining reproductive health.

    “A growing body of peer-reviewed research shows significant declines in sperm counts over the past decades, and pretending this isn’t a serious trend is irresponsible,” he said. “The data is real, the stakes are high and ignoring it doesn’t make it go away.”

    Lamb said the analyses from Swan and her co-authors had a major weakness in their methodology. They assumed that laboratories in different parts of the world were collecting and testing semen in the same way, she said, when in fact the methods likely varied.

    Swan stood by her team’s results, telling NBC News in an email that they accounted for differences in methodologies across studies, as well as the challenges of getting accurate sperm counts.

    Lundy, of the Cleveland Clinic, said measuring sperm counts can be hard to do consistently. The count itself can go up and down depending on the frequency of ejaculation, time of year, or whether someone is injured or has a fever.

    His analysis last year found a subtle decline in sperm count among men in the U.S. from 1970 to 2018, but one that likely wouldn’t impact fertility in real life.

    “What it has done is showed that there’s no cause for widespread panic for the typical U.S. male,” Lundy said.

    Vaping, cigarette smoking and binge drinking can also decrease sperm counts.Christopher Furlong / Getty Images file

    Researchers who believe sperm counts are declining said it might be influenced by two factors: obesity and environmental chemicals.

    “We know that obesity is one of the strongest predictors of serum testosterone, and also to a lesser extent, of sperm counts,” said Jorge Chavarro, a professor of nutrition and epidemiology at the Harvard T.H. Chan School of Public Health. In particular, he said, obesity can decrease the secretion of key hormones in the brain that regulate reproduction in both men and women.

    A 2023 study also found an association between exposure to pesticides and significantly lower sperm concentrations.

    Pesticides “can imitate or interfere with naturally occurring hormones, and those hormones are necessary for the production of healthy sperm,” said Melissa Perry, the study’s author and dean of the College of Public Health at George Mason University.

    Kennedy has blamed both factors for falling sperm counts in the U.S., but some researchers say it’s too soon to draw a link to national or worldwide trends.

    Vaping, cigarette smoking and binge drinking can also decrease sperm counts. (Research on marijuana use is mixed, with one study suggesting it can increase sperm counts and another finding the opposite.) Testosterone replacement therapy — a treatment that has exploded in popularity among young men looking to feel more energized or to increase their sex drive — can also shut off sperm production entirely.

    “Men on testosterone are almost uniformly azoospermic and totally infertile, and sometimes that is only partially reversible if they’ve been on high-dose testosterone for many years,” Lundy said.

    Kennedy himself told Newsmax in 2023 that he takes testosterone replacement as part of an “anti-aging protocol.” Most doctors say the treatment should be reserved for people with a medical condition and is not meant to counteract the normal aging process or increase vitality in young men.

    While sperm count can influence fertility, it’s not the only factor. The shape and movement of sperm can also have an effect, since slow or misshapen sperm can have trouble reaching or fertilizing an egg. Swollen veins in the scrotum called varicoceles can play a role, too.

    “If you lined up 100 men who are having fertility problems, about 35% or 36% would have varicoceles,” said Dr. Stanton Honig, a urology professor at Yale School of Medicine. “That’s one of the most treatable, reversible causes of male factor infertility.”

    Honig said doctors tend to get concerned when sperm counts fall below 15 million sperm per milliliter of semen, or less than 31% of sperm being mobile. But even then, a suboptimal sperm count doesn’t necessarily mean an inability to reproduce.

    “You have to get to pretty low sperm concentration levels before you start seeing an impact on a couple’s ability to become pregnant,” Chavarro said.

    Even men with high sperm counts may struggle to have kids. Up to half of male infertility cases have an unknown cause, according to a 2007 study.

    Lundy said the issue deserves more attention to better understand men’s health — not because of any fears about humanity dying out.

    “This is not the end of our species as we know it,” he said.

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  • Can adults make new brain cells? New study may finally settle one of neuroscience’s greatest debates

    Can adults make new brain cells? New study may finally settle one of neuroscience’s greatest debates

    Researchers say they have found clear evidence that the human brain can keep making new neurons well into adulthood, potentially settling decades of controversy.

    This new neuron growth, or “neurogenesis,” takes place in the hippocampus, a critical part of the brain involved in learning, memory and emotions.

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  • Cheese Giving You Nightmares? New Study Prompts PETA Remedy With ‘Rest Easy, Go Vegan’ Ads – PETA

    1. Cheese Giving You Nightmares? New Study Prompts PETA Remedy With ‘Rest Easy, Go Vegan’ Ads  PETA
    2. Cheese may really be giving you nightmares, scientists find  Frontiers
    3. Is lactose intolerance linked to nightmares?  Geo.tv
    4. From Scrooge to science: how dairy might disrupt your sleep and dreams  The Conversation
    5. Cheese Giving You Nightmares? New Study Prompts PETA Remedy With ‘Rest Easy, Go Vegan’ Ads in Albany  PETA

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  • Both standard and ultrahigh resolution modes effective for PCCT CCTA

    Both standard and ultrahigh resolution modes effective for PCCT CCTA

    Both standard and ultrahigh resolution modes are effective for coronary CT angiography (CCTA) performed by photon-counting detector CT (PCCT) — but ultrahigh has an extra advantage in patients with severe coronary artery disease, researchers have found.

    The findings will help clinicians optimize protocols for PCCT CCTA, according to a team led by Mengzhen Wang, MD, of Shanghai Jiao Tong University School of Medicine in China.

    “For coronary CTA performed by photon-counting detector (PCD) CT, diagnostic performance for significant stenosis is optimized through acquisition in ultrahigh resolution (UHR) mode with reconstruction at 0.2-mm slice thickness,” the American Journal of Roentgenology noted in a statement about the study, which was published July 3.

    CCTA is a widely used tool for evaluating the presence and severity of coronary artery disease, but its diagnostic efficacy is lower in patients with extensive coronary artery calcification, the team explained. That’s where PCCT comes in: The technology offers higher contrast-to-noise ratio and spatial resolution than conventional CT imaging.

    In their study, Wang and colleagues assessed the diagnostic performance for detecting stenosis on CCTA performed by PCCT with various standard resolution and ultrahigh resolution protocols. The group used invasive coronary angiography (ICA) as the reference standard.

    The research included a total of 122 inpatients who underwent CCTA between October 2023 and October 2024; of these, 61 patients underwent exams with a standard resolution protocol and 61 with an ultrahigh resolution protocol. The patients also underwent ICA.

    The exams were reconstructed in the following manner:

    • Standard resolution: “SRnormal” and “SRVNCa” (virtual noncalcium) image sets, both using 0.6-mm slice thickness and Bv40 kernel.
    • Ultrahigh resolution: “UHRnormal” (0.6-mm slice thickness, Bv40) and “UHRthin” (0.2-mm slice thickness, Bv64) image sets.

    Two radiologists measured the diameters of any stenoses; these were considered significant at a threshold equal to or greater than 50%.  

    Per-segment CCTA interpretation performance by reader

    Measure

    Reader 1

    Reader 2

    SRnormal

    Sensitivity

    92.9%

    92.9%

    Specificity

    89.9%

    88.8%

    Accuracy

    90.5%

    89.6%

    SRVNCa (virtual calcium)

    Sensitivity

    92.9%

    93.5%

    Specificity

    91.6%

    92.3%

    Accuracy

    91.9%

    92.5%

    UHRnormal

    Sensitivity

    96%

    96%

    Specificity

    92.4%

    91.6%

    Accuracy

    93%

    92.2%

    UHRthin

    Sensitivity

    100%

    100%

    Specificity

    98.6%

    98.9%

    Accuracy

    98.8%

    99%

    72-year-old female participant with heart failure. Patient underwent coronary CTA by photon-counting detector CTA, acquired in UHR mode. Agatston score was 1,615. Heart rate was 59 beats/min. (A) Reconstructed UHRnormal image. Proximal RCA shows calcified plaque. Inset shows cross-section of RCA at level of thin line traversing vessel. (B) Reconstructed UHRthin image. Proximal LCX shows calcified plaque. Inset shows cross-section of RCA at level of thin line traversing vessel. Insets show less blooming artifact from calcified plaque for UHRthin than for UHRnormal. Stenosis at site of calcification was measured as 60% for UHRnormal and 30% for UHRthin. (C) Image from subsequent invasive coronary angiography shows 30% stenosis of RCA (arrow). UHR = ultrahigh resolution. Images and caption courtesy of the AJR.

    The key finding of the work was that both standard resolution and ultrahigh resolution PCCT achieved high diagnostic performance for significant stenosis using ICA as the reference standard — although the team did note that “the superior diagnostic performance of UHR mode was most evident in patients with heavily calcified vessels,” writing that “radiology practices could consider prioritization of UHR mode for patients with known extensive coronary calcification or with strong clinical suspicion for severe CAD.”

    The complete study can be found here.

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  • Emerging PET Insights on Neuroinflammation with Progressive Apraxia of Speech (PAOS) and Parkinson-Plus Syndrome

    Emerging PET Insights on Neuroinflammation with Progressive Apraxia of Speech (PAOS) and Parkinson-Plus Syndrome

    Neuroinflammation may play a significant role in positron emission tomography (PET) detection of progressive apraxia of speech (PAOS) and determining whether there is coexisting Parkinson-plus syndrome, according to new research presented at the Society for Nuclear Medicine and Molecular Imaging (SNMMI) conference.

    For the prospective study, researchers reviewed data from (11C)ER176 TSPO PET and (18F)flortaucipir tau PET scans obtained for 25 patients with PAOS (including 13 patients with Parkinson-plus syndrome) and 30 healthy control participants.

    In a recent interview at the SNMMI conference, lead study author Ryota Satoh, Ph.D., said the study findings revealed significantly greater neuroinflammation for patients with PAOS in regions such as the premotor cortex, basal ganglia and the superior, middle and inferior frontal gyri in contrast to PET scans from healthy control participants.

    While the uptake pattern on PET scans was limited to the left frontal gyri and bilateral premotor cortex in patients with PAOS and no Parkinson-plus syndrome, Dr. Satoh noted that those with PAOS and Parkinson-plus syndrome had broader uptake that extended to prefrontal, temporal and parietal cortices.

    “These results suggest that tau-associated neuroinflammation could occur in early stages of the disease, but the degree of neuroinflammation increases and spreads once the patient develop Parkinson-plus syndrome,” noted Dr. Satoh, an assistant professor in the Department of Radiology at Mayo Clinic in Rochester, Minn.

    (Editor’s note: For additional coverage of the SNMMI conference, click here.)

    While acknowledging the need for larger cohort longitudinal studies, Dr. Satoh said the neuroinflammation may emerge as a key consideration in disease treatment in this patient population.

    “Our results suggest that inflammation plays an important role in the disease mechanisms of PAOS, and it is related to underlying … tau. These results indicate an inflammation mechanism could be the target of the treatment of this disease,” added Dr. Satoh.

    (Editor’s note: For related content, see “FDA Clears Emerging Brain PET System,” “Can Brain MRI-Based Connectome Mapping Predict the Progression of Parkinson’s Disease?” and “Researcher Presents First Non-Invasive Images or Alpha-Synuclein in the Brain.”)

    For more insights from Dr. Satoh, watch the video below.

    Reference

    1. Satoh R, Utianski RL, Duffy JR, et al. Neuroinflammatory (11C)ER176 TSPO PET profile with colocalized tau uptake in progressive apraxia of speech. Presented at the Society for Nuclear Medicine and Molecular Imaging (SNMMI) conference, June 21-24, 2025, New Orleans. Available at: https://www.xcdsystem.com/snmmi/program/B95p18u/index.cfm?pgid=2402&sid=46745&mobileappid=4674500000 .

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  • Drinking Water May Lower Stroke Risk

    Drinking Water May Lower Stroke Risk

    • A new study found that drinking at least 6 cups of water a day significantly lowers stroke risk.
    • Being well-hydrated helps blood flow more easily and lowers blood sugar.
    • If you’re not drinking enough water, swap other beverages for plain water throughout the day.

    Being well-hydrated has many benefits beyond quenching thirst. Hydration is necessary for your body to function properly, and you can’t survive more than about three days without hydration. Your brain is also highly dependent on water. Even slight dehydration can negatively impact mood, memory and concentration. 

    Studies have shown a link between hydration status and blood flow. Essentially, when we’re dehydrated, our blood is thicker and doesn’t flow as easily through our blood vessels. This also increases the chances of it being “clumpy.” And clumpy blood has been linked to stroke and heart attacks, the No. 1 killer of American adults. Researchers also point out evidence that drinking enough water is associated with a reduced risk of hypertension, diabetes and obesity. Plus, there’s even a link between adequate water intake and lower levels of inflammation.

    Researchers from China wanted to know if drinking enough plain water could reduce stroke risk, and if so, what threshold of water intake makes a difference. They took a closer look at data from a long-term American study, the National Health and Nutrition Examination Survey (NHANES), to find answers and published their findings in the Journal of Stroke and Cerebrovascular Diseases. Let’s break down what they found.

    How Was This Study Conducted?

    Researchers pulled data from the years 1999 to 2020 of NHANES. They included adults over the age of 20 at baseline, with an average age of 49. More than 29,000 people were included in this analysis, and about 48% were male. 

    For NHANES, participants completed 24-hour diet recalls that included daily water intake. Since recalling what we ate the previous day leaves room for error, researchers took the average of two 24-hour recalls for increased accuracy. Participants were then placed into one of four quartiles (groups) based on their water intake. Those in quartile 1 (Q1) had the lowest water intake, and those in Q4 had the highest.

    Information on stroke was gathered from participants answering the question, “Has a doctor or other health professional ever told you that you had a stroke?” Almost 1,300 participants answered yes. 

    Variables that would be adjusted for during statistical analyses included age, sex, ethnicity/race, education and income levels, smoking status, alcohol intake, BMI, physical activity, calorie consumption, hydration consumption, high blood pressure, diabetes, high cholesterol and heart disease. 

    What Did This Study Find?

    After adjusting for all relevant demographic variables—plus total calorie and hydration consumption—the group that drank the most water had a 25% lower risk of stroke compared to those who drank the least. 

    Researchers also found an association between stroke risk and the amount of plain water intake. Those who drank less than approximately 1,400 mL of water per day—which is equal to about 6 cups or 47 fluid ounces of water—had a significantly higher risk of stroke. 

    Some limitations of this study include the 24-hour dietary recalls, which, as previously mentioned, leave room for error and bias in participants recalling what they ate. Also, because NHANES is an observational study, researchers cannot establish causation, just that there is an association between those who drink more plain water and a lower risk of stroke. Lastly, only plain water was considered, so it’s unknown if other types of water, like sparkling water, might have the same associations with stroke risk as plain water.

    How Does This Apply to Real Life?

    The amount of water needed for each person depends on several factors, including sex, age, activity levels and medications. And while it is person-dependent, there are some general guidelines to provide a framework for you. For example, the National Academies of Sciences, Engineering and Medicine recommends that men drink 15.5 cups of water per day and women drink 11.5 cups per day.

    Eating hydrating foods can also add to your hydration status. Nosh on watermelon, cucumbers, apples, grapefruit, okra, tomatoes and plain Greek yogurt to help stay hydrated. 

    If your blood sugar tends to run on the high side, consider your hydrating habits. When blood is thicker and more viscous, blood sugar is more concentrated. Drinking plenty of water helps thin it out and can help lower blood sugar levels. 

    With that said, too much of a good thing is, well, too much, and you can overhydrate. Referred to as hyponatremia or water intoxication, flooding your body with too much water dilutes the body’s electrolytes. And while rare, it can lead to death. Some signs you might be over imbibing on the H2O include clear urine, bloating, nausea, brain fog, headache and confusion. 

    Do an assessment of your water intake. It can help to track it over several days and take the average of them. Are you coming close to the recommended 11.5 to 15.5 cups a day? It also helps to assess the color of your urine. Ideally, it’s about the color of diluted lemonade. This color suggests that you’re getting enough water into you. If it’s dark yellow or amber, it’s a sign that you’re not and are dehydrated, so drink up. 

    It’s important to note that certain medications, supplements and foods can affect urine color. For example, if you’ve eaten beets, your urine might be red-tinged. And supplements that contain B vitamins can give you bright yellow pee. 

    One strategy to get you started is to swap one of your typical drinks for water. So if you tend to grab soda throughout the day, start by exchanging one soda a day with water. Next week, swap out two sodas and so on. 

    If you can’t stomach all that plain water, try flavoring it with fruits, vegetables and herbs. Lemon, Cucumber and Mint Infused Water is clean and refreshing on the palate. Or add berries or a splash of 100% juice to up the flavor quotient of plain water. Another trick is to try the water at different temperatures. Some people can tolerate it better when it’s ice cold. 

    Besides staying well-hydrated, there are other lifestyle factors that may help lower your stroke risk, too. Following a heart-healthy diet plan or an eating style like the Mediterranean diet will go a long way toward getting the nutrients you need for a healthy heart and brain. So stock up on plenty of fruits, veggies, whole grains, legumes, nuts, seeds, lean proteins and healthy fats. Engaging in regular physical activity, dealing with what’s stressing you out and getting plenty of quality sleep also play large, connected roles in preventing diseases like stroke. 

    Our Expert Take

    According to this study, drinking less than 6 cups a day of plain water increases your risk of stroke. It is unknown whether plain carbonated waters, like sparkling water, reduce stroke risk, but they do contribute to overall hydration. Flavor your water with fruit, veggies and herbs if you have a difficult time imbibing plain water. Also consider your whole-health picture and other lifestyle habits that contribute to heart and brain health, which play a role in stroke prevention.

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  • XPR1 Found Key in Ovarian Cancer Growth Regulation

    XPR1 Found Key in Ovarian Cancer Growth Regulation

    A recent study published in Genes & Diseases reveals a novel role of XPR1 in promoting ovarian cancer growth by regulating autophagy and MHC-I expression. The research, conducted by scientists from Chongqing Medical University, identifies XPR1 as a critical factor influencing the aggressiveness of ovarian cancer through its interaction with LAMP1 and the PI3K/Akt/mTOR signaling pathway. These findings shed light on new therapeutic targets for ovarian cancer, a malignancy known for its poor prognosis and resistance to immune checkpoint inhibitors.

    The study highlights that XPR1 expression is significantly increased in ovarian cancer tissues compared to normal ovarian tissues. This heightened expression correlates with advanced cancer stages, reduced overall survival, and lower progression-free survival. Through CRISPR-Cas9 screening, researchers identified XPR1 as a potential regulator of autophagy. Subsequent experiments confirmed that silencing XPR1 decreased ovarian cancer cell proliferation and metastasis, while overexpression led to the opposite effect, indicating its role in promoting cancer growth.

    Further analysis revealed that XPR1 interacts with LAMP1, a key lysosomal-associated membrane protein, and regulates its expression. This interaction modulates autophagy flux, particularly during the early phase of autophagy and to some extent during the lysosomal phase. Silencing XPR1 led to increased lysosome formation and autophagy, while its overexpression suppressed these processes. The study demonstrated that XPR1 regulates autophagy through the PI3K/Akt/mTOR pathway, inhibiting autophagy flux and thereby promoting ovarian cancer cell survival.

    In addition to autophagy regulation, the study identified a critical role of XPR1 in immune evasion. MHC-I molecules, crucial for CD8+ T cell recognition and tumor cell killing, were found to be regulated by XPR1 through autophagy. Silencing XPR1, combined with the use of chloroquine, an autophagy inhibitor, significantly enhanced the presence of MHC-I molecules on ovarian cancer cells. This combination treatment reduced tumor growth in mouse models, suggesting that targeting XPR1 alongside autophagy inhibition could improve the effectiveness of immunotherapy in ovarian cancer.

    These findings suggest that XPR1 serves as a potential therapeutic target for ovarian cancer, especially in cases resistant to PD-1 and CTLA-4 inhibitors. Targeting XPR1, either through direct silencing or by using autophagy inhibitors, may offer a novel approach to enhance the immune response against ovarian cancer. The study provides a foundation for future research into the use of autophagy modulators in combination with immune checkpoint inhibitors to improve treatment outcomes.

    /Public Release. This material from the originating organization/author(s) might be of the point-in-time nature, and edited for clarity, style and length. Mirage.News does not take institutional positions or sides, and all views, positions, and conclusions expressed herein are solely those of the author(s).View in full here.

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  • Ridding cells of mitochondria sheds light on their function

    Ridding cells of mitochondria sheds light on their function

    DALLAS – July 03, 2025 – By using a genetic technique developed at UT Southwestern Medical Center that forces cells to rid themselves of mitochondria, researchers are gaining new insights into the function of these critical organelles. Their findings, published in Cell, add to fundamental knowledge about the role of mitochondria in cells and evolution and could eventually lead to new treatments for patients with mitochondrial diseases such as Leigh syndrome and Kearns-Sayre syndrome, which can affect numerous organ systems.

    “Our new tool allows us to study how changes in mitochondrial abundance and the mitochondrial genome affect cells and organisms,” said Jun Wu, Ph.D., Associate Professor of Molecular Biology at UT Southwestern. Dr. Wu co-led the study with Daniel Schmitz, Ph.D., a former graduate student in the Wu Lab who is now a postdoctoral fellow at the University of California, Berkeley.

    Mitochondria are organelles found in the cells of most eukaryotic organisms, including animals, plants, and fungi, whose cells contain a membrane-bound nucleus and other membrane-bound organelles. They have their own genetic material, passed down exclusively through females of a species. Mitochondria are thought to have originated as prokaryotic cells – which lack membrane-bound organelles – and to have invaded ancestral eukaryotic cells and formed a symbiotic relationship with them.

    Researchers have long known that these organelles serve as cells’ powerhouses, generating the energetic molecule adenosine triphosphate that fuels all cellular operations. However, recent studies have shown mitochondria play direct roles in regulating cell death, differentiating stem cells into other cell types, transmitting molecular signals, aging, and developmental timing.

    Although mitochondria appear to perform many of these roles through “crosstalk” with the DNA in a cell’s nucleus, how they perform this function – and what happens if this crosstalk ceases – has been unknown.

    To help answer these questions, Dr. Wu, Dr. Schmitz, and their colleagues took advantage of a pathway called mitophagy that cells normally use to dispose of old or damaged mitochondria. Using genetic engineering, the researchers forced cells to degrade all their mitochondria – a process known as “enforced mitophagy.”

    The researchers used this process on human pluripotent stem cells (hPSCs), a type of cell typically formed early in development that can differentiate into other cell types. Although this alteration caused the cells to stop dividing, the researchers unexpectedly found that the mitochondria-depleted cells could survive in petri dishes up to five days. They had similar results with different types of mouse stem cells and hPSCs harboring a pathogenic mitochondrial DNA mutation, suggesting enforced mitophagy can be a viable tool for depleting mitochondria across species and cell types.

    To determine how removing mitochondria affected the hPSCs, the researchers assessed nuclear gene expression. They found that 788 genes became less active and 1,696 became more active. An analysis of the affected genes showed the hPSCs appeared to retain their ability to form other cell types and that they could partially compensate for the lack of mitochondria, with proteins encoded by nuclear genes taking over energy production and certain other functions typically performed by the missing organelles.

    Then the researchers, in an attempt to better understand crosstalk between mitochondria and the cell nucleus, fused hPSCs with pluripotent stem cells (PSCs) from humans’ closest primate relatives – including chimpanzee, bonobo, gorilla, and orangutan. This formed “composite” cells with two nuclear genomes and two sets of mitochondria, one from each species. These composite cells selectively removed all non-human primate mitochondria, leaving behind only human mitochondria.

    Next, using enforced mitophagy, the scientists created hPSCs devoid of human mitochondria and fused them to non-human primate PSCs, again creating cells carrying nuclear genomes from both species, but this time only non-human mitochondria. An analysis of composite cells containing either human or non-human mitochondria showed that the mitochondria were largely interchangeable despite millions of years of evolutionary separation, causing only subtle differences in gene expression within the composite nucleus.

    Interestingly, the genes that differed in activity among cells harboring human and non-human mitochondria were mostly linked to brain development or neurological diseases. This raises the possibility that mitochondria may play a role in the brain differences between humans and our closest primate relatives. However, Dr. Wu said, more research – especially studies comparing neurons made from these composite PSCs – will be needed to better understand these differences.

    Finally, the researchers studied how depleting mitochondria might affect development in whole organisms. They used a genetically encoded version of enforced mitophagy to reduce the amount of mitochondria in mouse embryos, then implanted them into surrogate mothers to develop. Embryos missing more than 65% of their mitochondria failed to implant in their surrogate’s uterus. However, those missing about a third of their mitochondria experienced delayed development, catching up to normal mitochondrial numbers and a typical developmental timeline by 12.5 days after fertilization.

    Together, the researchers say, these results serve as starting points for new lines of research into the different roles mitochondria play in cellular function, tissues and organ development, aging, and species evolution. They plan to use enforced mitophagy to continue studying these organelles in a variety of capacities.

    Other UTSW researchers who contributed to this study are Peter Ly, Ph.D., Assistant Professor of Pathology and Cell Biology; Daiji Okamura, Ph.D., Visiting Assistant Professor of Molecular Biology; Seiya Oura, Ph.D., and Leijie Li, Ph.D., postdoctoral researchers; Yi Ding, Ph.D., Research Associate; Rashmi Dahiya, Ph.D., Senior Research Associate; Emily Ballard, B.S., graduate student researcher; and Masahiro Sakurai, Ph.D., Research Scientist.

    Dr. Wu is a Virginia Murchison Linthicum Scholar in Medical Research. Drs. Wu and Ly are members of the Harold C. Simmons Comprehensive Cancer Center.

    About UT Southwestern Medical Center 

    UT Southwestern, one of the nation’s premier academic medical centers, integrates pioneering biomedical research with exceptional clinical care and education. The institution’s faculty members have received six Nobel Prizes and include 25 members of the National Academy of Sciences, 23 members of the National Academy of Medicine, and 14 Howard Hughes Medical Institute Investigators. The full-time faculty of more than 3,200 is responsible for groundbreaking medical advances and is committed to translating science-driven research quickly to new clinical treatments. UT Southwestern physicians provide care in more than 80 specialties to more than 140,000 hospitalized patients, more than 360,000 emergency room cases, and oversee nearly 5.1 million outpatient visits a year.


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